Expression of S100A4 by a variety of cell types present in the tumor microenvironment of human breast cancer

2007 ◽  
Vol 121 (7) ◽  
pp. 1433-1444 ◽  
Author(s):  
Teresa Cabezón ◽  
Julio E. Celis ◽  
Inge Skibshøj ◽  
Jörg Klingelhöfer ◽  
Mariam Grigorian ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Microenvironment ◽  
Human Breast Cancer ◽  
Cell Types ◽  
Human Breast

scholarly journals P01.06 Spatially-resolved, highly multiplexed biomarker analysis of cancerous and normal human breast tissues

2020 ◽  
Vol 8 (Suppl 2) ◽  
pp. A10.2-A11
Author(s):  
O Braubach ◽  
S Basak ◽  
M Gallina ◽  
W Lee ◽  
J Kim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Microenvironment ◽  
Human Breast Cancer ◽  
Cell Types ◽  
Normal Breast ◽  
Human Breast ◽  
Spatially Resolved ◽  
Biomarker Analysis ◽  
Part Time ◽  
Breast Tissues

BackgroundBreast cancer has a high incidence rate and there is a need to develop new diagnostic tools and treatment regimens. Progress has, unfortunately, been slow and new technologies are urgently needed to generate a comprehensive understanding of breast cancer biology. Highly multiplexed imaging is an emerging tool that can help to unravel the complexities of the tumor microenvironment. This technology enables the detection of tens of biomarkers within a tissue specimen, and allows comprehensive cell phenotyping, biomarker quantification and spatial localization at cellular resolution. Such measurements can, in turn, provide insights into disease mechanisms and identify potential treatment targets. We demonstrate the development of a breast cancer specific CO-Detection by indEXing (CODEX®) panel that allows simultaneous in situ imaging of more than 30+ antibody markers.Materials and MethodsCODEX® relies on a DNA-based tagging approach, whereby antibodies are labeled with specific oligonucleotide tags (barcodes) and dye-oligonucleotides (reporters) are iteratively hybridized and dehybridized across multiple cycles. This process is completely automated through the CODEX® instrument and readily deployable on commercially available fluorescence microscopy systems. Using a 30+ antibody CODEX® panel, we compared formalin-fixed paraffin embedded (FFPE) human breast cancer tissues at different stages of disease progression with normal breast tissues. Our antibody panel was designed to detect cancer cells as well as non-malignant cells in order to comprehensively survey the tumor microenvironment and normal control tissues. Data were analyzed using the CODEX® software suite to identify key cell types and analyze spatial associations.ResultsOur analyses revealed more than 20 distinct cell types in human breast cancer and normal tissues. Cell populations, biomarker expression and cellular spatial distributions differed distinctly between cancerous and normal breast tissues. Differences were robust, repeatedly observed and indicative of altered cellular milieus in normal versus cancerous breast tissues.ConclusionsCollectively, these data establish CODEX® as a readily deployable and practical tool for spatially-resolved, highly multiplexed biomarker analysis of human FFPE samples.Disclosure InformationO. Braubach: A. Employment (full or part-time); Significant; Akoya Biosciences. S. Basak: A. Employment (full or part-time); Significant; Akoya Biosciences. M. Gallina: A. Employment (full or part-time); Significant; Akoya Biosciences. W. Lee: A. Employment (full or part-time); Significant; Akoya Biosciences. J. Kim: A. Employment (full or part-time); Significant; Akoya Biosciences. C. Hempel: A. Employment (full or part-time); Significant; Akoya Biosciences. E. Williams: A. Employment (full or part-time); Significant; Akoya Biosciences. O. Shang: A. Employment (full or part-time); Significant; Akoya Biosciences. B. Cheung: A. Employment (full or part-time); Significant; Akoya Biosciences. J. Kennedy-Darling: A. Employment (full or part-time); Significant; Akoya Biosciences.

scholarly journals Activation of the Na+/H+ antiport is not required for epidermal growth factor-dependent gene expression, growth inhibition or proliferation in human breast cancer cells

1989 ◽  
Vol 257 (1) ◽  
pp. 151-157 ◽  
Author(s):  
J G Church ◽  
G B Mills ◽  
R N Buick
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Cell Types ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Epidermal Growth

Mitogen interaction with specific receptors in many cell types leads to activation of the Na+/H+ antiport and a resultant cytoplasmic alkalinization. Since amiloride inhibits both Na+/H+ exchange and cell proliferation, it has been hypothesized that activation of the antiport is an obligatory requirement for mitogenesis. However, concentrations of amiloride which inhibit the antiport also inhibit other cellular processes, including protein synthesis and phosphorylation. We have used an epidermal growth factor (EGF) receptor gene-amplified human breast cancer cell line, the growth of which is inhibited by high levels of EGF in culture (MDA-468) and a variant, the growth of which is stimulated by EGF (MDA-468-S4), along with two potent amiloride analogues to examine whether activation of the Na+/H+ antiport and cytoplasmic alkalinization is necessary for both EGF-dependent effects to occur. At concentrations of the amiloride analogues which block Na+/H+ exchange in both cell types by 76-98%, the EGF-dependent alterations in [3H]thymidine incorporation or induction in c-myc or c-fos gene transcription were unaltered. These results were confirmed by a lack of effect of the amiloride analogues on both the growth-stimulatory and growth-inhibitory effects on EGF in an anchorage-independent growth assay. Similarly, in pH-altered media that prevented normal cytoplasmic alkalinization, the response of both MDA-468 and MDA-468-S4 to EGF activation was unaltered. In addition, activation of the Na+/H+ antiport alone was not sufficient to induce c-myc and c-fos transcription in either cell type. Taken together, these data suggest that neither the Na+/H+ antiport nor cytoplasmic alkalinization are necessary or sufficient for either EGF-dependent growth stimulation or growth inhibition in MDA-468 human breast cancer cells.

MiR-302b improves chemotherapy efficacy in human breast cancer and affects tumor microenvironment

2016 ◽  
Vol 61 ◽  
pp. S41
Author(s):  
A. Cataldo ◽  
I. Plantamura ◽  
E. D’Ippolito ◽  
S. Baroni ◽  
D. Palmieri ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Microenvironment ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Chemotherapy Efficacy

Expression of human endogenous retroviral sequences: Differences between individuals and cell types. Increased expression in a human breast cancer

1995 ◽  
Vol 121 (S1) ◽  
pp. S3-S3 ◽  
Author(s):  
Jonas Blomberg ◽  
Patrik Medstrand ◽  
Hong Yin ◽  
Marie-Louise Andersson ◽  
Mats Lindeskog ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Cell Types ◽  
Human Breast

scholarly journals Talin2 regulates invasion of human breast cancer MDA‑MB‑231 cells via alteration of the tumor microenvironment

2019 ◽  
Author(s):  
Zhengwei Wen ◽  
Yingfan Liang ◽  
Shengming Deng ◽  
Lilin Zou ◽  
Xiaofan Xie ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Microenvironment ◽  
Human Breast Cancer ◽  
Human Breast
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