scholarly journals A novel role for placental leucine aminopeptidase (P-LAP) as a determinant of chemoresistance in endometrial carcinoma cells

2005 ◽  
Vol 118 (6) ◽  
pp. 1390-1394 ◽  
Author(s):  
Chihiro Kondo ◽  
Kiyosumi Shibata ◽  
Mikio Terauchi ◽  
Hiroaki Kajiyama ◽  
Kazuhiko Ino ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Leucine Aminopeptidase ◽  
Carcinoma Cells ◽  
Aminopeptidase P

Placental leucine aminopeptidase (P-LAP) expression is associated with chemosensitivity in human endometrial carcinoma

2004 ◽  
Vol 95 (2) ◽  
pp. 307-313 ◽  
Author(s):  
Kiyosumi Shibata ◽  
Fumitaka Kikkawa ◽  
Chihiro Kondo ◽  
Yayoi Mizokami ◽  
Hiroaki Kajiyama ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Leucine Aminopeptidase ◽  
Aminopeptidase P

Immunocytochemical localization of β1-4 galactosyltransferase in endometrial carcinoma cells

1990 ◽  
Vol 48 (3) ◽  
pp. 944-945
Author(s):  
Ichiro Yamamoto ◽  
Toshiaki Tachibana ◽  
Hiroko Maruyama ◽  
Noriyuki Komatsu ◽  
Hiroyuki Kuramoto ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Cell Lines ◽  
Endometrial Adenocarcinoma ◽  
Protein A ◽  
Rabbit Antiserum ◽  
Carcinoma Cells ◽  
Affinity Column ◽  
Antibody Technique ◽  
Galactosyl Transferase ◽  
C Terminus

We have paid attention to the alteration of glycosyltransferase in carcinoma cells, because it might be related to the malignancy of the cells. In this connection, localization of β1-4 galactosyl transferase (β1-4 Gal T) in human endometrial carcinoma cells was examined immunocytochemically using two kinds of cell lines, each of which showed different degree of differentiation.An antibody was purified from the rabbit antiserum against the synthetic peptide, IFNRLVFRGMSC (W89) of human β1-4 Gal T coupled with KLH (keyhole limpet hemocyanine) by protein A column and peptide-affinity column chromatography. The anti-W89 serum reacts to the C-terminus of human β 1-4 Gal T and to both membrane-bound and soluble forms of the enzyme. Cell line of well differentiated endometrial adenocarcinoma (I) and that of poorly differentiated endometrial adenocarcinoma (50B) were cultivated respectively in MEM medium containing 15% FCS and 2 mM glutamine for 4 d at 37°C under 5% CO2. The cells were fixed in a mixture of 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M Soerensen’s phosphate buffer (pH 7.4) at 4°C for 30 min, washed with PBS, then freezed and thawed. The indirect method of the peroxidase- labeled antibody technique was used for immunocytochemistry of both LM and TEM on the cell lines. The cells were dehydrated in ethanol and embedded in TAAB 812. Ultrathin sections were observed under a TEM, JEM-100S.

Construction of a novel asymmetric imidazole-cored AIE probe for ratiometric imaging of endogenous leucine aminopeptidase

2021 ◽  
Author(s):  
Xueyan Huang ◽  
Qian Lei ◽  
Shuai Huang ◽  
Hongliang Zeng ◽  
Bin Feng ◽  
...  
Keyword(s):  
Leucine Aminopeptidase ◽  
Aminopeptidase P ◽  
Ratiometric Imaging

We reported a rational strategy to deliberately construct the first asymmetric tetraarylimidazole-based AIE probe, integrating AIE behavior in synergy with ESIPT character to ratiometric imaging of endogenous LAP for the...

scholarly journals Overexpression of Indoleamine 2,3-Dioxygenase in Human Endometrial Carcinoma Cells Induces Rapid Tumor Growth in a Mouse Xenograft Model

2008 ◽  
Vol 14 (22) ◽  
pp. 7251-7259 ◽  
Author(s):  
Norio Yoshida ◽  
Kazuhiko Ino ◽  
Yoshiyuki Ishida ◽  
Hiroaki Kajiyama ◽  
Eiko Yamamoto ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Endometrial Carcinoma ◽  
Xenograft Model ◽  
Carcinoma Cells ◽  
Mouse Xenograft ◽  
Indoleamine 2,3 Dioxygenase ◽  
Mouse Xenograft Model

Role of Estrogen and Estrogen-Related Growth Factor in the Mechanism of Hormone Dependency of Endometrial Carcinoma Cells

Oncology ◽  
1998 ◽  
Vol 55 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Hiroki Hata ◽  
Mieko Hamano ◽  
Jun Watanabe ◽  
Hiroyuki Kuramoto
Keyword(s):  
Growth Factor ◽  
Endometrial Carcinoma ◽  
Carcinoma Cells ◽  
Hormone Dependency

scholarly journals Benzotriazole Enhances Cell Invasive Potency in Endometrial Carcinoma Through CTBP1-Mediated Epithelial-Mesenchymal Transition

2017 ◽  
Vol 44 (6) ◽  
pp. 2357-2367 ◽  
Author(s):  
Yiquan Wang ◽  
Chencheng Dai ◽  
Cheng Zhou ◽  
Wenqu Li ◽  
Yujia Qian ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Epithelial Mesenchymal Transition ◽  
Expression Patterns ◽  
Underlying Mechanism ◽  
Carcinoma Cells ◽  
Mesenchymal Transition ◽  
Invasion And Migration ◽  
Female Reproductive Health ◽  
Gene Microarray Analysis ◽  
And Migration

Background/Aims: Benzotriazole (BTR) and its derivatives, such as intermediates and UV stabilizers, are important man-made organic chemicals found in everyday life that have been recently identified as environmental toxins and a threat to female reproductive health. Previous studies have shown that BTR could act as a carcinogen by mimicking estrogen. Environmental estrogen mimics could promote the initiation and development of female cancers, such as endometrial carcinoma, a type of estrogenic-sensitive malignancy. However, there is little information on the relationship between BTR and endometrial carcinoma. In this study, we aimed to demonstrate the biological function of BTR in endometrial carcinoma and explored the underlying mechanism. Methods: The CCK-8 assay was performed to detect cell viability; transwell-filter assay was used to assess cell invasion; gene microarray analysis was employed to determine gene expression patterns in response to BTR treatment; western blotting and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were carried out to detect the expression levels of BTR-related genes. Results: Our data showed that BTR could induce the invasion and migration of endometrial carcinoma cells (Ishikawa and HEC-1-B). In addition, BTR increased the expression level of CTBP1, which could enhance the epithelial-mesenchymal transition (EMT) in cancer cells. Moreover, CTBP1 silencing reversed the effect of BTR on EMT progression in endometrial carcinoma cells. Conclusion: This study indicates that BTR could act as a carcinogen to promote the development of endometrial carcinoma mainly through CTBP1-mediated EMT, which deserves more attention.

scholarly journals Prognostic significance of Notch signalling molecules and their involvement in the invasiveness of endometrial carcinoma cells

2012 ◽  
Vol 60 (5) ◽  
pp. 826-837 ◽  
Author(s):  
Yuko Mitsuhashi ◽  
Akiko Horiuchi ◽  
Tsutomu Miyamoto ◽  
Hiroyasu Kashima ◽  
Akihisa Suzuki ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Prognostic Significance ◽  
Notch Signalling ◽  
Carcinoma Cells ◽  
Signalling Molecules

Plasma angiotensin I and serum placental leucine aminopeptidase (P-LAP) in pre-eclampsia

1985 ◽  
Vol 236 (3) ◽  
pp. 165-172 ◽  
Author(s):  
S. Mizutani ◽  
H. Akiyama ◽  
O. Kurauchi ◽  
H. Taira ◽  
O. Narita ◽  
...  
Keyword(s):  
Leucine Aminopeptidase ◽  
Angiotensin I ◽  
Plasma Angiotensin ◽  
Aminopeptidase P
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