Photodynamic therapy with WST09 (Tookad): Quantitative studies in normal colon and transplanted tumours

2006 ◽  
Vol 118 (2) ◽  
pp. 477-482 ◽  
Author(s):  
Josephine H. Woodhams ◽  
Alexander J. MacRobert ◽  
Marco Novelli ◽  
Stephen G. Bown
Keyword(s):  
Photodynamic Therapy ◽  
Normal Colon ◽  
Quantitative Studies

scholarly journals Photodynamic therapy with phthalocyanine sensitisation: quantitative studies in a transplantable rat fibrosarcoma

1987 ◽  
Vol 55 (4) ◽  
pp. 389-395 ◽  
Author(s):  
CJ Tralau ◽  
AJ MacRobert ◽  
PD Coleridge-Smith ◽  
H Barr ◽  
SG Bown
Keyword(s):  
Photodynamic Therapy ◽  
Quantitative Studies

scholarly journals Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal Carcinoma

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Raduan Hage ◽  
Juliana Ferreira ◽  
Vanderlei Salvador Bagnato ◽  
José Dirceu Vollet-Filho ◽  
Hélio Plapler
Keyword(s):  
Photodynamic Therapy ◽  
Colorectal Carcinoma ◽  
Fluorescence Spectroscopy ◽  
Wistar Rats ◽  
Fluorescence Spectra ◽  
Target Organ ◽  
Hematoporphyrin Derivative ◽  
Spectroscopy Technique ◽  
Normal Colon

This study aimed to investigate the pharmacokinetics of a hematoporphyrin derivative in colonic tumors induced by dimethylhydrazine and adjacent normal colon in Wistar rats using anin vivofluorescence spectroscopy technique. In conventional clinical application of photodynamic therapy, the interval between photosensitizer (PS) administration and lesion illumination is often standardized without taking into account variations due to the type or localization of the tumor and intrinsic differences in the microcirculation and vascular permeability of each target organ. The analysis of the fluorescence spectra was based on the intensity of porphyrin emission band centered at around 620 nm in normal colon and colon tumors. The photosensitizer fluorescence intensity rapidly grew for carcinoma and normal colon, reaching the maximum values 1 and 3 hours after PS injection, respectively. Data presented here allow us to verify that the best compromise between selectivity and drug concentration for colon carcinoma in rats took place in the interval between 1 to 4 h after PS injection.

Optimisation of Illumination for Photodynamic Therapy With mTHPC on Normal Colon and a Transplantable Tumour in Rats

2002 ◽  
Vol 17 (2) ◽  
pp. 101-109 ◽  
Author(s):  
H. Tsutsui ◽  
A.J. MacRobert ◽  
A. Curnow ◽  
A. Rogowska ◽  
G. Buonaccorsi ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Normal Colon ◽  
Transplantable Tumour

scholarly journals Photodynamic therapy with porphyrin and phthalocyanine sensitisation: Quantitative studies in normal rat liver

1986 ◽  
Vol 54 (1) ◽  
pp. 43-52 ◽  
Author(s):  
S G Bown ◽  
C J Tralau ◽  
P D Coleridge Smith ◽  
D Akdemir ◽  
T J Wieman
Keyword(s):  
Photodynamic Therapy ◽  
Rat Liver ◽  
Quantitative Studies ◽  
Normal Rat

Residual Gas Reaction in the Electron Microscope: II The Design of a Gas-Control Chamber for Quantitative Observations

1969 ◽  
Vol 27 ◽  
pp. 82-83
Author(s):  
Chester J. Calbick ◽  
Richard E. Hartman
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Local Environment ◽  
Chamber Pressure ◽  
Objective Lens ◽  
Ion Pump ◽  
Quantitative Studies ◽  
Precise Knowledge ◽  
Differential Pumping ◽  
And Control

Quantitative studies of the phenomenon associated with reactions induced by the electron beam between specimens and gases present in the electron microscope require precise knowledge and control of the local environment experienced by the portion of the specimen in the electron beam. Because of outgassing phenomena, the environment at the irradiated portion of the specimen is very different from that in any place where gas pressures and compositions can be measured. We have found that differential pumping of the specimen chamber by a 4" Orb-Ion pump, following roughing by a zeolite sorption pump, can produce a specimen-chamber pressure 100- to 1000-fold less than that in the region below the objective lens.

Comparative strengths and weaknesses of peroxidase and colloidal gold in pre- and post-embedding immunolabeling techniques

1987 ◽  
Vol 45 ◽  
pp. 1002-1005
Author(s):  
D. R. Abrahamson ◽  
P. L. St.John ◽  
E. W. Perry
Keyword(s):  
Electron Microscopy ◽  
Horseradish Peroxidase ◽  
Light Microscopy ◽  
Colloidal Gold ◽  
Light Microscope ◽  
Ultrastructural Localization ◽  
The Other ◽  
Quantitative Studies ◽  
Dense Suspension ◽  
Antigen Localization

Antibodies coupled to tracers for electron microscopy have been instrumental in the ultrastructural localization of antigens within cells and tissues. Among the most popular tracers are horseradish peroxidase (HRP), an enzyme that yields an osmiophilic reaction product, and colloidal gold, an electron dense suspension of particles. Some advantages of IgG-HRP conjugates are that they are readily synthesized, relatively small, and the immunolabeling obtained in a given experiment can be evaluated in the light microscope. In contrast, colloidal gold conjugates are available in different size ranges and multiple labeling as well as quantitative studies can therefore be undertaken through particle counting. On the other hand, gold conjugates are generally larger than those of HRP but usually can not be visualized with light microscopy. Concern has been raised, however, that HRP reaction product, which is exquisitely sensitive when generated properly, may in some cases distribute to sites distant from the original binding of the conjugate and therefore result in spurious antigen localization.

Construction of a near-infrared responsive upconversion nanoplatform against hypoxic tumors via NO-enhanced photodynamic therapy

Nanoscale ◽  
2020 ◽  
Vol 12 (14) ◽  
pp. 7875-7887 ◽  
Author(s):  
Ying Lan ◽  
Xiaohui Zhu ◽  
Ming Tang ◽  
Yihan Wu ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Upconversion Nanoparticles

A near-infrared (NIR) activated theranostic nanoplatform based on upconversion nanoparticles (UCNPs) is developed in order to overcome the hypoxia-associated resistance in photodynamic therapy by photo-release of NO upon NIR illumination.

Porphyrin-functionalized coordination star polymers and their potential applications in photodynamic therapy

2019 ◽  
Vol 10 (45) ◽  
pp. 6116-6121 ◽  
Author(s):  
Tan Ji ◽  
Lei Xia ◽  
Wei Zheng ◽  
Guang-Qiang Yin ◽  
Tao Yue ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Self Assembly ◽  
Star Polymers ◽  
New Family ◽  
Potential Applications

We present a new family of porphyrin-functionalized coordination star polymers prepared through combination of coordination-driven self-assembly and post-assembly polymerization. Their self-assembly behaviour in water and potential for photodynamic therapy were demonstrated.

A nitroreductase-activatable near-infrared theranostic photosensitizer for photodynamic therapy under mild hypoxia

2020 ◽  
Vol 56 (43) ◽  
pp. 5819-5822
Author(s):  
Jing Zheng ◽  
Yongzhuo Liu ◽  
Fengling Song ◽  
Long Jiao ◽  
Yingnan Wu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Triplet State ◽  
Near Infrared ◽  
Mild Hypoxia

In this study, a near-infrared (NIR) theranostic photosensitizer was developed based on a heptamethine aminocyanine dye with a long-lived triplet state.

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