A novel nonstop mutation in TYMP does not induce nonstop mRNA decay in a MNGIE patient with severe neuropathy

2011 ◽  
Vol 32 (4) ◽  
Author(s):  
Javier Torres‐Torronteras ◽  
Agustí Rodriguez‐Palmero ◽  
Tomàs Pinós ◽  
Anna Accarino ◽  
Antoni L. Andreu ◽  
...  
Keyword(s):  
Mrna Decay ◽  
Nonstop Mrna Decay ◽  
Nonstop Mutation

scholarly journals tmRNA determinants required for facilitating nonstop mRNA decay

RNA ◽  
2006 ◽  
Vol 12 (12) ◽  
pp. 2187-2198 ◽  
Author(s):  
P. Mehta ◽  
J. Richards ◽  
A. W. Karzai
Keyword(s):  
Mrna Decay ◽  
Nonstop Mrna Decay

MOLECULAR BIOLOGY: Skiing Toward Nonstop mRNA Decay

Science ◽  
2002 ◽  
Vol 295 (5563) ◽  
pp. 2221-2222 ◽  
Author(s):  
L. E. Maquat
Keyword(s):  
Molecular Biology ◽  
Mrna Decay ◽  
Nonstop Mrna Decay

A functional involvement of ABCE1, eukaryotic ribosome recycling factor, in nonstop mRNA decay in Drosophila melanogaster cells

Biochimie ◽  
2014 ◽  
Vol 106 ◽  
pp. 10-16 ◽  
Author(s):  
Isao Kashima ◽  
Masaki Takahashi ◽  
Yoshifumi Hashimoto ◽  
Eri Sakota ◽  
Yoshikazu Nakamura ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Mrna Decay ◽  
Ribosome Recycling Factor ◽  
Eukaryotic Ribosome ◽  
Nonstop Mrna Decay ◽  
Functional Involvement

scholarly journals Distinct tmRNA sequence elements facilitate RNase R engagement on rescued ribosomes for selective nonstop mRNA decay

2014 ◽  
Vol 42 (17) ◽  
pp. 11192-11202 ◽  
Author(s):  
Krithika Venkataraman ◽  
Hina Zafar ◽  
A. Wali Karzai
Keyword(s):  
Mrna Decay ◽  
Rnase R ◽  
Nonstop Mrna Decay ◽  
Sequence Elements

Chapter 17 Studying tmRNA‐Mediated Surveillance and Nonstop mRNA Decay

2008 ◽  
pp. 329-358 ◽  
Author(s):  
Thomas Sundermeier ◽  
Zhiyun Ge ◽  
Jamie Richards ◽  
Daniel Dulebohn ◽  
A. Wali Karzai
Keyword(s):  
Mrna Decay ◽  
Nonstop Mrna Decay

scholarly journals Genetic interactions between [PSI+] and nonstop mRNA decay affect phenotypic variation

2005 ◽  
Vol 102 (29) ◽  
pp. 10244-10249 ◽  
Author(s):  
M. A. Wilson ◽  
S. Meaux ◽  
R. Parker ◽  
A. van Hoof
Keyword(s):  
Phenotypic Variation ◽  
Mrna Decay ◽  
Genetic Interactions ◽  
Nonstop Mrna Decay

scholarly journals Nonsense mRNA suppression via nonstop decay

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Joshua A Arribere ◽  
Andrew Z Fire
Keyword(s):  
Mrna Decay ◽  
Stop Codon ◽  
Premature Stop Codon ◽  
Nonsense Mediated Decay ◽  
Stop Codons ◽  
Nonstop Mrna Decay ◽  
Rna Fragments ◽  
Nonsense Mediated Mrna Decay ◽  
Key Steps

Nonsense-mediated mRNA decay is the process by which mRNAs bearing premature stop codons are recognized and cleared from the cell. While considerable information has accumulated regarding recognition of the premature stop codon, less is known about the ensuing mRNA suppression. During the characterization of a second, distinct translational surveillance pathway (nonstop mRNA decay), we trapped intermediates in nonsense mRNA degradation. We present data in support of a model wherein nonsense-mediated decay funnels into the nonstop decay pathway in Caenorhabditis elegans. Specifically, our results point to SKI-exosome decay and pelota-based ribosome removal as key steps facilitating suppression and clearance of prematurely-terminated translation complexes. These results suggest a model in which premature stop codons elicit nucleolytic cleavage, with the nonstop pathway disengaging ribosomes and degrading the resultant RNA fragments to suppress ongoing expression.

scholarly journals NONU-1 encodes a conserved endonuclease required for mRNA translation surveillance

2019 ◽  
Author(s):  
Marissa L. Glover ◽  
A. Max. Burroughs ◽  
Thea A. Egelhofer ◽  
Makena N. Pule ◽  
L. Aravind ◽  
...  
Keyword(s):  
Mrna Decay ◽  
Domain Architecture ◽  
Mrna Translation ◽  
Mrna Levels ◽  
Mechanistic Studies ◽  
C Elegans ◽  
Endonucleolytic Cleavage ◽  
Nonstop Mrna Decay ◽  
Unknown Factor ◽  
Cellular Translation

ABSTRACTCellular translation surveillance rescues ribosomes that stall on problematic mRNAs. During translation surveillance, endonucleolytic cleavage of the problematic mRNA is a critical step in rescuing stalled ribosomes. However, the nuclease(s) responsible remain unknown. Here we identify NONU-1 as a novel endoribonuclease required for translation surveillance pathways including No-Go and Nonstop mRNA Decay. We show that: (1) NONU-1 reduces Nonstop and No-Go mRNA levels; (2) NONU-1 contains an Smr RNase domain required for mRNA decay and with properties similar to the unknown endonuclease; and (3) the domain architecture and catalytic residues of NONU-1 are conserved throughout metazoans and eukaryotes, respectively. We extend our results inC. elegansto homologous factors inS. cerevisiae, showing conservation of function of the NONU-1 protein across billions of years of evolution. Our work establishes the identity of a previously unknown factor critical to translation surveillance and will inform mechanistic studies at the intersection of translation and mRNA decay.

Trans-Translation:  The tmRNA-Mediated Surveillance Mechanism for Ribosome Rescue, Directed Protein Degradation, and Nonstop mRNA Decay†

Biochemistry ◽  
2007 ◽  
Vol 46 (16) ◽  
pp. 4681-4693 ◽  
Author(s):  
Daniel Dulebohn ◽  
Jennifer Choy ◽  
Thomas Sundermeier ◽  
Nihal Okan ◽  
A. Wali Karzai
Keyword(s):  
Protein Degradation ◽  
Mrna Decay ◽  
Nonstop Mrna Decay ◽  
Surveillance Mechanism

scholarly journals Ribosomal ubiquitination facilitates mRNA cleavage and ribosome rescue during No-Go and Nonstop mRNA Decay

2021 ◽  
Author(s):  
Parissa C. Monem ◽  
Audrey L. Piatt ◽  
Nitin Vidyasagar ◽  
Marissa L. Glover ◽  
Thea A. Egelhofer ◽  
...  
Keyword(s):  
Ribosomal Proteins ◽  
Mrna Decay ◽  
Critical Role ◽  
Mrna Degradation ◽  
C Elegans ◽  
N Terminus ◽  
Nonstop Mrna Decay ◽  
Cellular Machinery ◽  
Translation Signals

During translational surveillance, ribosomes play a critical role in detecting problematic mRNAs and signaling cellular machinery to repress the offending messages. Prior work has shown that problematic mRNAs identified by two surveillance pathways (Nonstop and No-Go mRNA Decay) are detected by ribosome collisions and subsequent ribosomal ubiquitination, yet how ribosomal ubiquitination leads to repression has remained unclear. Here, we deploy C. elegans to unravel the series of coordinated events during Nonstop and No-Go mRNA Decay. We probe the metazoan SKI RNA helicase complex to uncover functionally significant residues and reveal divergence of the SKI-exosome interface. We define a functional requirement for ubiquitination on at least two ribosomal proteins during No-Go mRNA Decay, and illustrate how ubiquitination recruits the endonuclease NONU-1 via CUE domains and the ribosome rescue factor HBS-1 via its poorly characterized N-terminus. Our molecular characterization (1) underscores the importance of ribosomal ubiquitination in mRNA degradation, (2) shows similar and distinct genetic dependencies of factors in Nonstop and No-Go mRNA Decay, and (3) uncovers a conspicuous absence of distinct ribosomal stalls at No-Go mRNA Decay substrates. Our work demonstrates mechanisms by which translation signals to effectors of co-translational mRNA repression and has implications for the study of translation and ribosomal species in vivo.

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