Trans-Translation:  The tmRNA-Mediated Surveillance Mechanism for Ribosome Rescue, Directed Protein Degradation, and Nonstop mRNA Decay†

Biochemistry ◽  
2007 ◽  
Vol 46 (16) ◽  
pp. 4681-4693 ◽  
Author(s):  
Daniel Dulebohn ◽  
Jennifer Choy ◽  
Thomas Sundermeier ◽  
Nihal Okan ◽  
A. Wali Karzai
Keyword(s):  
Protein Degradation ◽  
Mrna Decay ◽  
Nonstop Mrna Decay ◽  
Surveillance Mechanism

scholarly journals tmRNA determinants required for facilitating nonstop mRNA decay

RNA ◽  
2006 ◽  
Vol 12 (12) ◽  
pp. 2187-2198 ◽  
Author(s):  
P. Mehta ◽  
J. Richards ◽  
A. W. Karzai
Keyword(s):  
Mrna Decay ◽  
Nonstop Mrna Decay

MOLECULAR BIOLOGY: Skiing Toward Nonstop mRNA Decay

Science ◽  
2002 ◽  
Vol 295 (5563) ◽  
pp. 2221-2222 ◽  
Author(s):  
L. E. Maquat
Keyword(s):  
Molecular Biology ◽  
Mrna Decay ◽  
Nonstop Mrna Decay

A functional involvement of ABCE1, eukaryotic ribosome recycling factor, in nonstop mRNA decay in Drosophila melanogaster cells

Biochimie ◽  
2014 ◽  
Vol 106 ◽  
pp. 10-16 ◽  
Author(s):  
Isao Kashima ◽  
Masaki Takahashi ◽  
Yoshifumi Hashimoto ◽  
Eri Sakota ◽  
Yoshikazu Nakamura ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Mrna Decay ◽  
Ribosome Recycling Factor ◽  
Eukaryotic Ribosome ◽  
Nonstop Mrna Decay ◽  
Functional Involvement

scholarly journals Distinct tmRNA sequence elements facilitate RNase R engagement on rescued ribosomes for selective nonstop mRNA decay

2014 ◽  
Vol 42 (17) ◽  
pp. 11192-11202 ◽  
Author(s):  
Krithika Venkataraman ◽  
Hina Zafar ◽  
A. Wali Karzai
Keyword(s):  
Mrna Decay ◽  
Rnase R ◽  
Nonstop Mrna Decay ◽  
Sequence Elements

Chapter 17 Studying tmRNA‐Mediated Surveillance and Nonstop mRNA Decay

2008 ◽  
pp. 329-358 ◽  
Author(s):  
Thomas Sundermeier ◽  
Zhiyun Ge ◽  
Jamie Richards ◽  
Daniel Dulebohn ◽  
A. Wali Karzai
Keyword(s):  
Mrna Decay ◽  
Nonstop Mrna Decay

scholarly journals Genetic interactions between [PSI+] and nonstop mRNA decay affect phenotypic variation

2005 ◽  
Vol 102 (29) ◽  
pp. 10244-10249 ◽  
Author(s):  
M. A. Wilson ◽  
S. Meaux ◽  
R. Parker ◽  
A. van Hoof
Keyword(s):  
Phenotypic Variation ◽  
Mrna Decay ◽  
Genetic Interactions ◽  
Nonstop Mrna Decay

scholarly journals Nonsense mRNA suppression via nonstop decay

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Joshua A Arribere ◽  
Andrew Z Fire
Keyword(s):  
Mrna Decay ◽  
Stop Codon ◽  
Premature Stop Codon ◽  
Nonsense Mediated Decay ◽  
Stop Codons ◽  
Nonstop Mrna Decay ◽  
Rna Fragments ◽  
Nonsense Mediated Mrna Decay ◽  
Key Steps

Nonsense-mediated mRNA decay is the process by which mRNAs bearing premature stop codons are recognized and cleared from the cell. While considerable information has accumulated regarding recognition of the premature stop codon, less is known about the ensuing mRNA suppression. During the characterization of a second, distinct translational surveillance pathway (nonstop mRNA decay), we trapped intermediates in nonsense mRNA degradation. We present data in support of a model wherein nonsense-mediated decay funnels into the nonstop decay pathway in Caenorhabditis elegans. Specifically, our results point to SKI-exosome decay and pelota-based ribosome removal as key steps facilitating suppression and clearance of prematurely-terminated translation complexes. These results suggest a model in which premature stop codons elicit nucleolytic cleavage, with the nonstop pathway disengaging ribosomes and degrading the resultant RNA fragments to suppress ongoing expression.

scholarly journals Nonsense-mediated mRNA decay and development: shoot the messenger to survive?

2010 ◽  
Vol 38 (6) ◽  
pp. 1500-1505 ◽  
Author(s):  
Marta Vicente-Crespo ◽  
Isabel M. Palacios
Keyword(s):  
Mrna Decay ◽  
Physiological Role ◽  
Cellular Growth ◽  
Nonsense Mutations ◽  
Rna Surveillance ◽  
Animal Development ◽  
Nonsense Mediated Mrna Decay ◽  
Surveillance Mechanism ◽  
Shed Light

NMD (nonsense-mediated mRNA decay) is a surveillance mechanism that degrades transcripts containing nonsense mutations, preventing the translation of potentially harmful truncated proteins. Although the mechanistic details of NMD are gradually being understood, the physiological role of this RNA surveillance pathway still remains largely unknown. The core NMD genes Upf1 (up-frameshift suppressor 1) and Upf2 are essential for animal viability in the fruitfly, mouse and zebrafish. These findings may reflect an important role for NMD during animal development. Alternatively, the lethal phenotypes of upf1 and upf2 mutants might be due to their function in NMD-independent processes. In the present paper, we describe the phenotypes observed when the NMD factors are mutated in various organisms, and discuss findings that might shed light on the function of NMD in cellular growth and development of an organism.

scholarly journals NONU-1 encodes a conserved endonuclease required for mRNA translation surveillance

2019 ◽  
Author(s):  
Marissa L. Glover ◽  
A. Max. Burroughs ◽  
Thea A. Egelhofer ◽  
Makena N. Pule ◽  
L. Aravind ◽  
...  
Keyword(s):  
Mrna Decay ◽  
Domain Architecture ◽  
Mrna Translation ◽  
Mrna Levels ◽  
Mechanistic Studies ◽  
C Elegans ◽  
Endonucleolytic Cleavage ◽  
Nonstop Mrna Decay ◽  
Unknown Factor ◽  
Cellular Translation

ABSTRACTCellular translation surveillance rescues ribosomes that stall on problematic mRNAs. During translation surveillance, endonucleolytic cleavage of the problematic mRNA is a critical step in rescuing stalled ribosomes. However, the nuclease(s) responsible remain unknown. Here we identify NONU-1 as a novel endoribonuclease required for translation surveillance pathways including No-Go and Nonstop mRNA Decay. We show that: (1) NONU-1 reduces Nonstop and No-Go mRNA levels; (2) NONU-1 contains an Smr RNase domain required for mRNA decay and with properties similar to the unknown endonuclease; and (3) the domain architecture and catalytic residues of NONU-1 are conserved throughout metazoans and eukaryotes, respectively. We extend our results inC. elegansto homologous factors inS. cerevisiae, showing conservation of function of the NONU-1 protein across billions of years of evolution. Our work establishes the identity of a previously unknown factor critical to translation surveillance and will inform mechanistic studies at the intersection of translation and mRNA decay.

scholarly journals Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during mitotic exit

2021 ◽  
Author(s):  
Lenno Krenning ◽  
Stijn Sonneveld ◽  
Marvin E Tanenbaum
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Protein Degradation ◽  
Translational Control ◽  
Mrna Decay ◽  
Gene Expression Regulation ◽  
G1 Phase ◽  
Cell Cycle Gene ◽  
Time Resolved ◽  
Cell Cycle Gene Expression

Accurate control of the cell cycle is critical for development and tissue homeostasis and requires precisely-timed expression of many genes. Cell cycle gene expression is regulated through transcriptional and translational control, as well as through regulated protein degradation. Here, we show that widespread and temporally-controlled mRNA decay acts as an additional mechanism for gene expression regulation during the cell cycle. We find that two waves of mRNA decay occur sequentially during the mitosis-to-G1 phase transition, and identify the deadenylase CNOT1 as a factor that contributes to mRNA decay during this cell cycle transition. Collectively, our data show that, akin to protein degradation, scheduled mRNA decay helps to reshape cell cycle gene expression as cells move from mitosis into G1 phase.

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