scholarly journals Dynamic three‐dimensional morphogenesis of intrahepatic bile ducts in mouse liver development

Hepatology ◽  
2015 ◽  
Vol 61 (3) ◽  
pp. 1003-1011 ◽  
Author(s):  
Yasuo Takashima ◽  
Maiko Terada ◽  
Masuyo Kawabata ◽  
Atsushi Suzuki
Keyword(s):  
Mouse Liver ◽  
Bile Ducts ◽  
Three Dimensional ◽  
Liver Development ◽  
Intrahepatic Bile Ducts

The onecut transcription factor HNF6 is required for normal development of the biliary tract

Development ◽  
2002 ◽  
Vol 129 (8) ◽  
pp. 1819-1828 ◽  
Author(s):  
Frédéric Clotman ◽  
Vincent J. Lannoy ◽  
Michael Reber ◽  
Silvia Cereghini ◽  
David Cassiman ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Biliary Tract ◽  
Bile Ducts ◽  
Normal Development ◽  
Intrahepatic Bile Duct ◽  
Liver Development ◽  
Prenatal Period ◽  
Intrahepatic Bile Ducts ◽  
Extrahepatic Bile Ducts ◽  
Duct Development

During liver development, hepatoblasts differentiate into hepatocytes or biliary epithelial cells (BEC). The BEC delineate the intrahepatic and extrahepatic bile ducts, and the gallbladder. The transcription factors that control the development of the biliary tract are unknown. Previous work has shown that the onecut transcription factor HNF6 is expressed in hepatoblasts and in the gallbladder primordium. We now show that HNF6 is also expressed in the BEC of the developing intrahepatic bile ducts, and investigate its involvement in biliary tract development by analyzing the phenotype of Hnf6–/– mice. In these mice, the gallbladder was absent, the extrahepatic bile ducts were abnormal and the development of the intrahepatic bile ducts was perturbed in the prenatal period. The morphology of the intrahepatic bile ducts was identical to that seen in mice whose Hnf1β gene has been conditionally inactivated in the liver. HNF1β expression was downregulated in the intrahepatic bile ducts of Hnf6–/– mice during development. Furthermore, we found that HNF6 can stimulate the Hnf1β promoter. We conclude that HNF6 is essential for differentiation and morphogenesis of the biliary tract and that intrahepatic bile duct development is controlled by a HNF6→HNF1β cascade.

693 THE CELL SOURCES AND DIFFERENTIATION OF INTRAHEPATIC BILE DUCTS DURING HUMAN LIVER DEVELOPMENT

2011 ◽  
Vol 54 ◽  
pp. S278-S279
Author(s):  
A. Gumerova ◽  
A. Kiassov ◽  
M. Titova ◽  
S. Abdulkhakov ◽  
M. Kaligin ◽  
...  
Keyword(s):  
Human Liver ◽  
Bile Ducts ◽  
Liver Development ◽  
Intrahepatic Bile Ducts ◽  
Cell Sources

A computer-generated three-dimensional view of the developing human biliary system

1996 ◽  
Vol 54 ◽  
pp. 626-627
Author(s):  
V. Vijayan ◽  
CEL Tan
Keyword(s):  
3D Reconstruction ◽  
Bile Ducts ◽  
Three Dimensional ◽  
Biliary System ◽  
3D Images ◽  
New Era ◽  
Biliary Epithelium ◽  
Ductal Plate ◽  
Intrahepatic Bile Ducts ◽  
Organ Systems

With the advent of computers, microscopy has entered an exciting new era of computerised three dimensional (3D) reconstruction. Using serial sections, it is now possible to generate complex 3D images, that can be rotated at various angles to study the organ systems of interest. In this project, the first of its kind, we have used computer generated 3D reconstruction to study the developing human biliary system.The human biliary system consists of the extra and intrahepatic components. The extrahepatic system develops from the embryonic hepatic diverticulum and is tubular from the start. The intrahepatic bile ducts arise from the Ductal Plate. The Ductal Plate comprises of sheets of biliary epithelium, with discontinuous luminal spaces, that appear within the mesenchyme along the portal vein branches. The Ductal Plate is remodelled into tubular definitive bile ducts by an orderly process of epithelial deletion and selection which occurs between 11-13 weeks of gestation

scholarly journals Liver ductal organoids reconstruct intrahepatic biliary trees in decellularized liver grafts.

2021 ◽  
Author(s):  
Katsuhiro Tomofuji ◽  
Ken Fukumitsu ◽  
Jumpei Kondo ◽  
Hiroshi Horie ◽  
Kenta Makino ◽  
...  
Keyword(s):  
Bile Duct ◽  
Bile Ducts ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Perfusion Culture ◽  
Biliary Tree ◽  
Liver Graft ◽  
Cell Distribution ◽  
Intrahepatic Bile Ducts ◽  
Decellularized Liver

Three-dimensional scaffolds decellularized from native organs are a promising technique to establish engineered liver grafts and overcome the current shortage of donor organs. However, limited sources of bile duct cells and inappropriate cell distribution in bioengineered liver grafts have hindered their practical application. Organoid technology is anticipated to be an excellent tool for the advancement of regenerative medicine. In the present study, we reconstructed intrahepatic bile ducts in a rat decellularized liver graft by recellularization with liver ductal organoids. Using an ex vivo perfusion culture system, we demonstrated the biliary characteristics of repopulated mouse liver organoids, which maintained bile duct markers and reconstructed biliary tree-like networks with luminal structures. We also established a method for the co-recellularization with engineered bile ducts and primary hepatocytes, revealing the appropriate cell distribution to mimic the native liver. We then utilized this model in human organoids to demonstrate the reconstructed bile ducts. Our results show that liver ductal organoids are a potential cell source for bile ducts from bioengineered liver grafts using three-dimensional scaffolds.

scholarly journals Notch signaling regulates formation of the three-dimensional architecture of intrahepatic bile ducts in mice

Hepatology ◽  
2009 ◽  
Vol 51 (4) ◽  
pp. 1391-1400 ◽  
Author(s):  
Erin E. Sparks ◽  
Kari A. Huppert ◽  
Melanie A. Brown ◽  
M. Kay Washington ◽  
Stacey S. Huppert
Keyword(s):  
Notch Signaling ◽  
Bile Ducts ◽  
Three Dimensional ◽  
Intrahepatic Bile Ducts

scholarly journals Intrahepatic bile ducts guide establishment of the intrahepatic nerve network in developing and regenerating mouse liver

Development ◽  
2018 ◽  
Vol 145 (9) ◽  
pp. dev159095 ◽  
Author(s):  
Naoki Tanimizu ◽  
Norihisa Ichinohe ◽  
Toshihiro Mitaka
Keyword(s):  
Mouse Liver ◽  
Bile Ducts ◽  
Nerve Network ◽  
Intrahepatic Bile Ducts

scholarly journals Improved Visualization of Intrahepatic Bile Ducts by Endoscopic Retrograde Balloon Catheter Cholangiography

1981 ◽  
Vol 194 (2) ◽  
pp. 171-175 ◽  
Author(s):  
SEIYO IKEDA ◽  
MASAO TANAKA ◽  
HIDEO YOSHIMOTO ◽  
HIDEAKI ITOH ◽  
FUMIO NAKAYAMA
Keyword(s):  
Bile Ducts ◽  
Balloon Catheter ◽  
Endoscopic Retrograde ◽  
Intrahepatic Bile Ducts
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