scholarly journals Transdifferentiation of rat hepatocytes into biliary cells after bile duct ligation and toxic biliary injury

Hepatology ◽  
2005 ◽  
Vol 41 (3) ◽  
pp. 535-544 ◽  
Author(s):  
George K. Michalopoulos ◽  
Lindsay Barua ◽  
William C. Bowen
Keyword(s):  
Bile Duct ◽  
Bile Duct Ligation ◽  
Rat Hepatocytes ◽  
Biliary Injury ◽  
Duct Ligation

Bile canalicular barrier function and expression of tight-junctional molecules in rat hepatocytes during common bile duct ligation

2001 ◽  
Vol 307 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Yasunari Takakuwa ◽  
Yasuo Kokai ◽  
Ken-ichi Sasaki ◽  
Hideki Chiba ◽  
Hirotoshi Tobioka ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Barrier Function ◽  
Bile Duct Ligation ◽  
Rat Hepatocytes ◽  
Common Bile Duct Ligation ◽  
Duct Ligation

Coexpression of periportal and perivenous enzymes in rat hepatocytes after experimental bile duct ligation: Comparison with intrasplenically transplanted hepatocytes

1996 ◽  
Vol 105 (4) ◽  
pp. 319-329 ◽  
Author(s):  
Lorraine Racine-Samson ◽  
Jean-Yves Scoazec ◽  
Alain Moreau ◽  
Laurence Christa ◽  
Dominique Bernuau ◽  
...  
Keyword(s):  
Bile Duct ◽  
Bile Duct Ligation ◽  
Rat Hepatocytes ◽  
Duct Ligation

Transcytotic vesicular carriers for polymeric IgA receptors accumulate in rat hepatocytes after bile duct ligation

1991 ◽  
Vol 98 (2) ◽  
pp. 205-216 ◽  
Author(s):  
J.M. Larkin ◽  
G.E. Palade
Keyword(s):  
Bile Duct ◽  
Serum Proteins ◽  
Bile Duct Ligation ◽  
Rat Hepatocytes ◽  
Frozen Sections ◽  
Transmission Electron ◽  
Duct Ligation ◽  
Sds Page ◽  
Vesicular Carriers

In rat hepatocytes, transcytotic vesicular carriers transport the mature 120 × 10(3) Mr form of the polymeric IgA receptor (pIgA-R), with or without its ligand, pIgA, from the sinusoidal to the biliary plasmalemma, where the ectodomain of the receptor is cleaved to produce an 80 × 10(3) Mr fragment that is secreted into the bile. Here we show that cholestasis induced by bile duct ligation results in the accumulation of transcytotic carriers, identified by the 120 × 10(3) Mr pIgA-R and pIgA, in the pericanalicular cytoplasm of hepatocytes. To determine the extent of pIgA-R accumulation, hepatic total microsomes (TM) were prepared from control and cholestatic rats. Solubilized TM proteins were separated by SDS-PAGE and receptor forms were detected by immunoblotting and autoradiography. Quantitative densitometry of these autoradiograms showed that after duct ligation the 120 × 10(3) Mr receptor accumulated to a level approximately threefold higher than the control. Concomitantly, immunologically related, novel 124, 90 and 80 × 10(3) Mr proteins (cholestatic antigens) became detectable. Immunoblot analyses of biliary and serum proteins showed that cholestasis resulted in: (1) a marked decrease in the concentrations of the 80 × 10(3) Mr receptor and pIgA in the bile, whereas albumin concentrations remained at control levels; and (2) a marked increase in the concentration of the 80 × 10(3) Mr receptor in the serum. Positive sites for pIgA-R were localized to the pericanalicular cytoplasm of hepatocytes by indirect immunofluorescence on semithin frozen sections in cholestatic hepatocytes. The sites were more numerous and the positive signal stronger than in controls. One day post-ligation, pIgA-positive sites were located to the same pericanalicular cytoplasm of hepatocytes; by three days, however, most pIgA appeared in sinusoidal endothelia and Kupffer cells. To validate the vesicular character of the receptor-positive sites, sham-operated and cholestatic livers were processed for either transmission electron microscopy (TEM) or immunogold localization of receptors on thin frozen sections. TEM verified the accumulation of pericanalicular vesicles in cholestatic hepatocytes. Immunogold tests localized pIgA-R to pleiomorphic, pericanalicular vesicles, which were increased in number, size and concentration of antigenic sites in cholestatic hepatocytes. These findings indicate that bile duct ligation provides a method for manipulating the in vivo transcytotic pathway and for accumulating previously unstudied transcytotic carriers in hepatocytes.

Effect of colchicine and phalloidin on the distribution of three plasma membrane antigens in rat hepatocytes: Comparison with bile duct ligation

Hepatology ◽  
1987 ◽  
Vol 7 (6) ◽  
pp. 1239-1248 ◽  
Author(s):  
Anne-Marie Durand-Schneider ◽  
Michèle Maurice ◽  
Micheline Dumont ◽  
Gérard Feldmann
Keyword(s):  
Plasma Membrane ◽  
Bile Duct ◽  
Bile Duct Ligation ◽  
Rat Hepatocytes ◽  
Duct Ligation ◽  
Membrane Antigens

Bid-mediated apoptosis does not contribute to cholestatic liver injury following bile duct ligation

2009 ◽  
Vol 47 (01) ◽  
Author(s):  
P Nalapareddy ◽  
S Schüngel ◽  
MP Manns ◽  
H Jaeschke ◽  
A Vogel
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Bile Duct Ligation ◽  
Duct Ligation ◽  
Cholestatic Liver Injury

HGF/c-Met and IL-6/gp130 cooperatively regulate liver homeostasis and antibacterial signalling after bile-duct-ligation

2010 ◽  
Vol 48 (01) ◽  
Author(s):  
A Giebeler ◽  
S Erschfeld ◽  
C Birchmeier ◽  
C Trautwein ◽  
KL Streetz
Keyword(s):  
Bile Duct ◽  
Bile Duct Ligation ◽  
Duct Ligation
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