Disturbed structural interactions between microfilaments and tight junctions in rat hepatocytes during extrahepatic cholestasis induced by common bile duct ligation

1996 ◽  
Vol 106 (6) ◽  
pp. 573-580 ◽  
Author(s):  
Ji-Ying Song ◽  
Jan Marle ◽  
Cornelis J. F. Noorden ◽  
Wilma M. Frederiks
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Tight Junctions ◽  
Bile Duct Ligation ◽  
Rat Hepatocytes ◽  
Common Bile Duct Ligation ◽  
Extrahepatic Cholestasis ◽  
Duct Ligation ◽  
Structural Interactions

Bile canalicular barrier function and expression of tight-junctional molecules in rat hepatocytes during common bile duct ligation

2001 ◽  
Vol 307 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Yasunari Takakuwa ◽  
Yasuo Kokai ◽  
Ken-ichi Sasaki ◽  
Hideki Chiba ◽  
Hirotoshi Tobioka ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Barrier Function ◽  
Bile Duct Ligation ◽  
Rat Hepatocytes ◽  
Common Bile Duct Ligation ◽  
Duct Ligation

scholarly journals Pathophysiology of Lung Injury Induced by Common Bile Duct Ligation in Mice

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94550 ◽  
Author(s):  
Fumiaki Shikata ◽  
Tomohisa Sakaue ◽  
Koh-ichi Nakashiro ◽  
Mikio Okazaki ◽  
Mie Kurata ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Lung Injury ◽  
Bile Duct Ligation ◽  
Common Bile Duct Ligation ◽  
Duct Ligation

scholarly journals A new model of reversible obstructive jaundice using rapidly absorbable suture materials

2012 ◽  
Vol 35 (6) ◽  
pp. 351 ◽  
Author(s):  
Nurettin Kahramansoy ◽  
Hayri Erkol ◽  
Edip E Yilmaz ◽  
Mustafa Şit ◽  
Fahri Yilmaz ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Obstructive Jaundice ◽  
Surgical Intervention ◽  
Bile Duct Ligation ◽  
Absorbable Suture ◽  
Suture Materials ◽  
Common Bile Duct Ligation ◽  
Duct Ligation ◽  
Morphologic Changes

Purpose: Reversible obstructive jaundice models have some limiting features, including the need for a second anaesthesia, re-laparotomy and surgical intervention after common bile duct ligation. The present study investigates the feasibility of a new application that can eliminate these limitations. Rapidly absorbable suture materials were used for ligation; therefore, spontaneous biliary decompression was anticipated by the self release of these rapidly degrading materials. Methods: Common bile ducts in Wistar Albino rats were ligated with silk, polyglytone 6211, or irradiated polyglactine 910 (n=7 for each group). Rats were grouped according to both the suture materials and the experiments termination date: 5 days (sham, silk5, polyglytone5, polyglactine5) and 21 days (silk21, polyglytone21, polyglactine21) after the ligation. Biochemical and morphologic changes of liver were assessed. Results: The group polyglactine21 showed significantly lower mean ALT, AST, GGT, total and direct bilirubin values when compared with the group polyglactine5 (p=0.004-0.037). Morphologic changes did not correlate with the biochemical amelioration. In the group polyglytone21, not only the biochemical but also the morphologic changes significantly ameliorated when compared with the group polyglytone5 (p=0.003-0.043). No procedure associated mortality was observed. Conclusion: Common bile duct ligation with polyglytone offers a new reversible model for prolonged obstructive jaundice which abolishes the need for relaparotomy and a second surgical intervention and significantly reduces mortality.

Characterization of inducible nature of MRP3 in rat liver

2000 ◽  
Vol 278 (3) ◽  
pp. G438-G446 ◽  
Author(s):  
Kotaro Ogawa ◽  
Hiroshi Suzuki ◽  
Tomoko Hirohashi ◽  
Toshihisa Ishikawa ◽  
Peter J. Meier ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Protein Level ◽  
Bile Duct Ligation ◽  
Organic Anion ◽  
Mrna Level ◽  
Common Bile Duct Ligation ◽  
Sd Rats ◽  
Anion Transporter ◽  
Duct Ligation

We found previously that expression of multidrug resistance-associated protein (MRP) 3 is induced in a mutant rat strain (Eisai hyperbilirubinemic rats) whose canalicular multispecific organic anion transporter (cMOAT/MRP2) function is hereditarily defective and in normal Sprague-Dawley (SD) rats after ligation of the common bile duct. In the present study, the inducible nature of MRP3 was examined, using Northern and Western blot analyses, in comparison with that of other secondary active [Na+-taurocholic acid cotransporting polypeptide (Ntcp), organic anion transporting polypeptide 1 (oatp1), and organic cation transporter (OCT1)] and primary active [P-glycoprotein (P-gp), cMOAT/MRP2, and MRP6] transporters. α-Naphthylisothiocyanate treatment and common bile duct ligation induced expression of P-gp and MRP3, whereas expression of Ntcp, oatp1, and OCT1 was reduced by the same treatment. Although expression of MRP3 was also induced by administration of phenobarbital, that of cMOAT/MRP2, MRP1, and MRP6 was not affected by any of these treatments. Moreover, the mRNA level of MRP3, but not that of P-gp, was increased in SD rats after administration of bilirubin and in Gunn rats whose hepatic bilirubin concentration is elevated because of a defect in the expression of UDP-glucuronosyl transferase. However, the MRP3 protein level was not affected by bilirubin administration. Although the increased MRP3 mRNA level was associated with the increased concentration of bilirubin and/or its glucuronides in mutant rats and in SD rats that had undergone common bile duct ligation or α-naphthylisothiocyanate treatment, we must assume that factor(s) other than these physiological substances are also involved in the increased protein level of MRP3.

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