Trifunctional bispecific antibody-induced tumor cell lysis of squamous cell carcinomas of the upper aerodigestive tract

Head & Neck ◽  
2005 ◽  
Vol 27 (5) ◽  
pp. 376-382 ◽  
Author(s):  
Silke S. Gronau ◽  
Michael Schmitt ◽  
Bernard Thess ◽  
Peter Reinhardt ◽  
Markus Wiesneth ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Squamous Cell ◽  
Bispecific Antibody ◽  
Cell Lysis ◽  
Squamous Cell Carcinomas ◽  
Aerodigestive Tract ◽  
Upper Aerodigestive Tract ◽  
Tumor Cell Lysis

CD19-/CD3-bispecific antibody of the BiTE class is far superior to tandem diabody with respect to redirected tumor cell lysis

2007 ◽  
Vol 44 (8) ◽  
pp. 1935-1943 ◽  
Author(s):  
Michael Mølhøj ◽  
Sandrine Crommer ◽  
Klaus Brischwein ◽  
Doris Rau ◽  
Mirnaalini Sriskandarajah ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Bispecific Antibody ◽  
Cell Lysis ◽  
Tumor Cell Lysis

Differential Expression of ras Signal Transduction Mediators in Verrucous and Squamous Cell Carcinomas of the Upper Aerodigestive Tract

2001 ◽  
Vol 125 (9) ◽  
pp. 1200-1203
Author(s):  
Julie L. Lessard ◽  
Robert A. Robinson ◽  
Henry T. Hoffman
Keyword(s):  
Signal Transduction ◽  
Squamous Cell ◽  
Gene Mutations ◽  
Squamous Cell Carcinomas ◽  
Aerodigestive Tract ◽  
Upper Aerodigestive Tract ◽  
Immunohistochemical Expression ◽  
Ras Gene ◽  
Extracellular Signal ◽  
Ras Signal Transduction

Abstract Context.—ras gene mutations and expression of its gene product have been described in verrucous and squamous cell carcinomas. Other downstream signal-transduction mediators, extracellular signal-regulated kinases 1 and 2 (ERK-1 and ERK-2) and Raf-1, have not yet been as extensively studied. Objective.—To determine patterns of expression of ERK-1, ERK-2, and Raf-1 in verrucous and squamous cell carcinomas of the upper aerodigestive tract. Design.—Seventeen verrucous carcinomas and 10 squamous cell carcinomas of the upper aerodigestive tract were examined for the immunohistochemical expression of ERK-1, ERK-2, and Raf-1 product. Results.—Raf-1 expression was intensely expressed in the most basal portions of the epithelium in verrucous carcinomas, but was minimally expressed in the suprabasalar areas. Anti-Raf-1 staining of the squamous cell carcinomas was diffuse and patchy throughout the tumor cells and was weak in intensity. There was no geographic preference of staining. The cytoplasmic expression of both ERK-1 and ERK-2 was predominantly negative in the most basal layers of the epithelium in the verrucous carcinomas, but was positive in the suprabasalar region of the epithelium. Immunohistochemical expression of ERK-1 and ERK-2 in the squamous carcinomas was diffuse throughout the tumor. Conclusion.—There is strong correlation of the geographic expression of these mediators of ras signal transduction in verrucous and squamous carcinomas, but the cause of these differences remains unclear at present. The expression of these mediator proteins may have potential for diagnosis, as well as in understanding the biologic behavior of these lesions.

A new format of bispecific antibody: highly efficient heterodimerization, expression and tumor cell lysis

2005 ◽  
Vol 296 (1-2) ◽  
pp. 95-101 ◽  
Author(s):  
Zhigang Xie ◽  
Ning Guo ◽  
Ming Yu ◽  
Meiru Hu ◽  
Beifen Shen
Keyword(s):  
Tumor Cell ◽  
Bispecific Antibody ◽  
Cell Lysis ◽  
Highly Efficient ◽  
Tumor Cell Lysis

Squamous Cell Carcinoma Variants of the Upper Aerodigestive Tract: A Comprehensive Review With a Focus on Genetic Alterations

2014 ◽  
Vol 138 (6) ◽  
pp. 731-744 ◽  
Author(s):  
Akeesha A. Shah ◽  
Susanne K. Jeffus ◽  
Edward B. Stelow
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Nuclear Protein ◽  
Genetic Alterations ◽  
Squamous Cell Carcinomas ◽  
Aerodigestive Tract ◽  
Treatment Modalities ◽  
Upper Aerodigestive Tract ◽  
Molecular Alterations

Context.— Squamous cell carcinoma of the upper aerodigestive tract is a heterogenous entity. Although conventional squamous cell carcinomas are easily recognized, the morphologic variants of squamous cell carcinoma can present a diagnostic challenge. Familiarity with these variants is necessary because many are associated with unique risk factors and are characterized by specific molecular alterations (eg, nuclear protein in testis midline carcinomas). Perhaps the most important distinction is in identifying viral-related from nonviral-related carcinomas. The accurate diagnosis of these variants is necessary for prognostic and therapeutic reasons. Objectives.— To provide a clinicopathologic overview and summary of the molecular alterations of the common squamous cell carcinoma variants, including verrucous, spindle cell, acantholytic, adenosquamous, basaloid, and papillary squamous cell carcinoma, as well as nuclear protein in testis midline carcinoma, and to discuss the distinguishing features of human papillomavirus- and Epstein-Barr virus-related squamous cell carcinomas. Data Sources.— Published peer-reviewed literature. Conclusions.— Familiarity with squamous cell carcinoma variants is essential for proper diagnosis and to guide appropriate clinical management. Further insight into the molecular alterations underlying those variants may lead to alterations in existing treatment approaches and to evolution of novel treatment modalities.

Papillary squamous cell carcinomas of the upper aerodigestive tract: A clinicopathologic and molecular study

Head & Neck ◽  
2000 ◽  
Vol 22 (4) ◽  
pp. 360-368 ◽  
Author(s):  
Patricia A. Suarez ◽  
Karen Adler-Storthz ◽  
Mario A. Luna ◽  
Adel K. El-Naggar ◽  
Fadi W. Abdul-Karim ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Molecular Study ◽  
Squamous Cell Carcinomas ◽  
Aerodigestive Tract ◽  
Upper Aerodigestive Tract

Synchronous and metachronous multicentric squamous cell carcinomas in the upper aerodigestive tract

1997 ◽  
Vol 47 (1) ◽  
pp. 68-72 ◽  
Author(s):  
Liang Shan ◽  
Yasushi Nakamura ◽  
Misa Nakamura ◽  
Zhiqiang Zhang ◽  
Xuefeng Jlng ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Aerodigestive Tract ◽  
Upper Aerodigestive Tract

Comparison of Quantitative DNA Measurements and Cytomorphology in Squamous Cell Carcinomas of the Upper Aerodigestive Tract with and without Lymph Node Metastases

1993 ◽  
Vol 102 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Hans-Juergen Welkoborsky ◽  
Jack L. Gluckman ◽  
Wolf J. Mann ◽  
James E. Freije
Keyword(s):  
Lymph Node ◽  
Squamous Cell ◽  
Dna Content ◽  
Lymph Node Metastases ◽  
Squamous Cell Carcinomas ◽  
Aerodigestive Tract ◽  
Upper Aerodigestive Tract ◽  
Malignancy Grade ◽  
Node Metastases ◽  
Dna Measurements

Cytologic smears of squamous cell carcinomas (UICC T2 through T4) from the mucosa of the upper aerodigestive tract without cervical lymph node metastases (n = 10) and with metastases (n = 20) were examined. Subsequently, the cytologic smears of the metastases (n = 20) obtained by fine-needle aspiration or following neck dissection were evaluated morphologically. Each specimen was then stained with Papanicolaou and Feulgen techniques, and quantitative DNA measurements were performed with an image analysis system. Morphologically, the primary tumors with metastases revealed an increased mean nuclear perimeter and mean nuclear area compared with tumors unassociated with metastases. On quantitative DNA measurements, the tumors with metastases had an increased DNA content, a higher 2c deviation index, and a higher DNA malignancy grade and increased number of aneuploid cells. The specimens obtained from the metastatic nodes yielded the highest values for mean DNA content, 2c deviation index, aneuploidy, and malignancy grade. The stem lines of metastasis were always nondiploid. These data indicate a positive correlation between aneuploid tumor cell clones in primary cancer and the manifestation of lymph node metastases.

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