Cooperative interaction of hepatocyte growth factor and neuregulin regulates Schwann cell migration and proliferation through Grb2-associated binder-2 in peripheral nerve repair

Glia ◽  
2017 ◽  
Vol 65 (11) ◽  
pp. 1794-1808 ◽  
Author(s):  
Yoon Kyoung Shin ◽  
So Young Jang ◽  
Seoug Hoon Yun ◽  
Yun Young Choi ◽  
Byeol-A Yoon ◽  
...  
Keyword(s):  
Cell Migration ◽  
Growth Factor ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Repair ◽  
Cooperative Interaction ◽  
Peripheral Nerve Repair ◽  
Schwann Cell Migration ◽  
Cell Migration And Proliferation ◽  
Hepatocyte Growth

scholarly journals Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

2021 ◽  
Vol 8 ◽  
Author(s):  
Ana M. Sandoval-Castellanos ◽  
Frederik Claeyssens ◽  
John W. Haycock
Keyword(s):  
Cell Migration ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Neurite Outgrowth ◽  
Nerve Regeneration ◽  
Nerve Repair ◽  
Donor Site ◽  
Nerve Tissue ◽  
Sustained Delivery ◽  
Schwann Cell Migration

Peripheral nerve injury is an important cause of disability, that can hinder significantly sensory and motor function. The clinical gold standard for peripheral nerve repair is the use of autografts, nevertheless, this method has limitations such as donor site morbidity. An emerging alternative to autografts are nerve guide conduits, which are used to entubulate the severed nerve and provide guidance for the directed regeneration of the nerve tissue. These nerve guide conduits are less effective than autografts, and to enhance their performance the incorporation of neurotrophins can be considered. To enable optimal nerve regeneration, it is important to continuously stimulate neurite outgrowth by designing a delivery system for the sustained delivery of neurotrophins. The aim of this study was to develop a novel bioactive surface on electrospun fibres to supply a sustained release of heparin bound NGF or BDNF electrostatically immobilised onto an amine functionalized surface to encourage neurite outgrowth and Schwann cell migration. The bioactive surface was characterised by XPS analysis and ELISA. To assess the effect of the bioactive surface on electrospun fibres, primary chick embryo dorsal root ganglia were used, and neurite outgrowth and Schwann cell migration were measured. Our results showed a significant improvement regarding nerve regeneration, with the growth of neurites of up to 3 mm in 7 days, accompanied by Schwann cells. We hypothesize that the physical guidance provided by the fibres along the sustained delivery of NGF or BDNF created a stimulatory environment for nerve regeneration. Our results were achieved by immobilising relatively low concentrations of neurotrophins (1 ng/ml), which provides a promising, low-cost, and scalable method to improve current nerve guide conduits.

LncRNA BC088259 promotes Schwann cell migration through Vimentin following peripheral nerve injury

Glia ◽  
2019 ◽  
Vol 68 (3) ◽  
pp. 670-679 ◽  
Author(s):  
Chun Yao ◽  
Yanping Chen ◽  
Jing Wang ◽  
Tianmei Qian ◽  
Wei Feng ◽  
...  
Keyword(s):  
Cell Migration ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Schwann Cell Migration

scholarly journals Betacellulin Regulates Peripheral Nerve Regeneration by Affecting Schwann Cell Migration and Axon Elongation

2021 ◽  
Author(s):  
Yaxian Wang ◽  
Fuchao Zhang ◽  
Yunsong Zhang ◽  
Qi Shan ◽  
Wei Liu ◽  
...  
Keyword(s):  
Cell Migration ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Cultured Cells ◽  
Axon Elongation ◽  
Schwann Cell Migration

Abstract Background Growth factors execute essential biological functions and affect various physiological and pathological processes, including peripheral nerve injury and regeneration. Our previous sequencing analysis found that betacellulin (Btc), an epidermal growth factor protein family member, showed elevated mRNA expressions in the nerve segment after rat peripheral nerve injury, implying the potential involvement of Btc during peripheral nerve repair. Methods Expression of Btc was examined in Schwann cells. The role of Btc in regulating Schwann cells was investigated by transfecting cultured cells with siRNA segment against Btc or exposed cultured cells with Btc recombinant protein, respectively. The biological functions of Schwann cell-secreted Btc on neurons were also determined. Moreover, the in vivo effect of Btc on Schwann cell migration and axon elongation after rat sciatic nerve injury were further evaluated.Results Immunostaining images and ELISA readings showed Btc was present in and secreted by Schwann cells. Transwell migration and wound healing observations showed that siRNA against Btc impeded Schwann cell migration while exogenous Btc advanced Schwann cell migration. Besides the regulating effect on Schwann cell phenotype, Btc secreted by Schwann cells might influence neuron behavior and affect axon length. In vivo evidence showed that Btc enhanced axonal regrowth and nerve regeneration after both rat sciatic nerve crush injury and transection injury. Conclusion Our findings demonstrated Btc-mediated Schwann cell-axon interactions, revealed the essential roles of Btc on Schwann cell migration and axon elongation, and implied the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.

scholarly journals Sustained Growth Factor Delivery Promotes Axonal Regeneration in Long Gap Peripheral Nerve Repair

2011 ◽  
Vol 17 (9-10) ◽  
pp. 1263-1275 ◽  
Author(s):  
Lauren E. Kokai ◽  
Dennis Bourbeau ◽  
Douglas Weber ◽  
Jedidiah McAtee ◽  
Kacey G. Marra
Keyword(s):  
Growth Factor ◽  
Peripheral Nerve ◽  
Axonal Regeneration ◽  
Nerve Repair ◽  
Growth Factor Delivery ◽  
Peripheral Nerve Repair ◽  
Sustained Growth ◽  
Long Gap

scholarly journals Nerve conduits and growth factor delivery in peripheral nerve repair

2007 ◽  
Vol 12 (2) ◽  
pp. 65-82 ◽  
Author(s):  
Lukas A. Pfister ◽  
Michaël Papaloïzos ◽  
Hans P. Merkle ◽  
Bruno Gander
Keyword(s):  
Growth Factor ◽  
Peripheral Nerve ◽  
Nerve Repair ◽  
Growth Factor Delivery ◽  
Peripheral Nerve Repair ◽  
Nerve Conduits
Sign in / Sign up

Export Citation Format

Share Document