scholarly journals A proposed lectin‐mediated mechanism to explain the in Vivo antihyperglycemic activity of γ‐conglutin from Lupinus albus seeds

2021 ◽  
Author(s):  
Madalena Grácio ◽  
João Rocha ◽  
Rui Pinto ◽  
Ricardo Boavida Ferreira ◽  
João Solas ◽  
...  
Keyword(s):  
Lupinus Albus ◽  
Antihyperglycemic Activity

Missing pieces in protein deposition and mobilization inside legume seed storage vacuoles: calcium and magnesium ions

2012 ◽  
Vol 22 (4) ◽  
pp. 249-258 ◽  
Author(s):  
Cláudia N. Santos ◽  
Marta M. Alves ◽  
Isabel T. Bento ◽  
Ricardo B. Ferreira
Keyword(s):  
Alkaline Earth ◽  
Storage Proteins ◽  
Lupinus Albus ◽  
Physiological Role ◽  
Experimental Conditions ◽  
Protein Storage Vacuoles ◽  
Aggregation And Disaggregation ◽  
Alkaline Earth Cations

AbstractDuring the maturation of dicotyledonous seeds, organic carbon, nitrogen and sulphur are stored in protein storage vacuoles (PSVs) as storage globulins. Several studies point to the coexistence of storage proteins with proteases responsible for their degradation inside PSVs. Different mechanisms have been proposed to explain why there is no proteolysis during this period. Protein aggregation to form large supramolecular structures resistant to proteolytic attack could be the reason. However, during germination, and particularly following its completion, the globulin aggregates must undergo disintegration to allow protease attack for protein reserve mobilization. Based on the well-described concentration-dependent ability of Ca2+ and Mg2+ to promote in vitro aggregation and disaggregation of globulins, we explored a possible role for these alkaline earth cations in globulin packaging and mobilization. Ca2+ and Mg2+ measurements in purified PSVs [6.37 μmol and 43.9 μmol g− 1 dry weight (DW) of cotyledons, respectively] showed the presence of these two alkaline earth cations within this compartment. To our knowledge, this is the first time that Ca2+ and Mg2+ have been quantified in purified PSVs from Lupinus albus seeds. Considering the importance of these two alkaline earth cations inside PSVs, which represent 14.6% and 60.7% of the total seed Mg2+and Ca2+, respectively, globulin aggregation and disaggregation profiles were assayed using experimental conditions closer to those that are physiologically present (proportion of Ca2+ and Mg2+, and acidic pH). Based on: (1) the high in vivo abundance of Ca2+ and Mg2+ inside PSVs; and (2) globulin aggregation and disaggregation profiles, together with structural and physiological evidence already reported in the literature, an important physiological role for Ca2+ and Mg2+ in globulin packaging and mobilization inside PSVs is suggested.

scholarly journals Germinated and Ungerminated Seeds Extract from TwoLupinusSpecies: Biological Compounds Characterization and In Vitro and In Vivo Evaluations

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Bogdan Andor ◽  
Corina Danciu ◽  
Ersilia Alexa ◽  
Istvan Zupko ◽  
Elena Hogea ◽  
...  
Keyword(s):  
Cinnamic Acid ◽  
Therapeutic Potential ◽  
Lupinus Albus ◽  
Biological Evaluation ◽  
Lupinus Angustifolius ◽  
Cinnamic Acid Derivatives ◽  
Natural Agents ◽  
Germinated Seeds

In recent years, nutraceuticals attracted a great amount of attention in the biomedical research due to their significant contribution as natural agents for prevention of various health issues. Ethanolic extracts from the ungerminated and germinated seeds ofLupinus albusL. andLupinus angustifoliusL. were analyzed for the content in isoflavones (genistein) and cinnamic acid derivatives. Additionally, the extracts were evaluated for antimicrobial, antiproliferative, and anti-inflammatory properties, using in vitro and in vivo tests. Germination proved to be a method of choice in increasing the amount of genistein and cinnamic acid derivatives in bothLupinus albusL. andLupinus angustifolius L.seeds. Biological evaluation of all vegetal extracts revealed a weak therapeutic potential for both ungerminated and germinated seeds.

Synthesis and in vivo Antihyperglycemic Activity of Nature-Mimicking Furanyl-2-pyranones in STZ-S Model.

ChemInform ◽  
2007 ◽  
Vol 38 (36) ◽  
Author(s):  
Fateh V. Singh ◽  
Sumit Chaurasia ◽  
Maya D. Joshi ◽  
Arvind K. Srivastava ◽  
Atul Goel
Keyword(s):  
Antihyperglycemic Activity ◽  
S Model

In vitro and in vivo bio-stimulatory properties of a Lupinus albus L. seed suspension

2011 ◽  
Vol 62 (3) ◽  
pp. 189 ◽  
Author(s):  
Elmarie van der Watt ◽  
Johan C. Pretorius
Keyword(s):  
Root Growth ◽  
Respiration Rate ◽  
Seedling Growth ◽  
Lupinus Albus ◽  
Positive Control ◽  
Seedling Root ◽  
Stimulatory Activity ◽  
Lupinus Albus L

During an extended screening program of plant extracts for bio-stimulatory activity, a seed suspension (SS) of Lupinus albus L. cv. Betsuhana White was identified as most promising. By testing a concentration range of SS using three in vitro bio-assays [i.e. respiration rate of monoculture yeast (Saccharomyces cerevisiae) cells, germination rate of seeds from selected crops, and subsequent seedling growth], 5 mg/L was identified as the optimal concentration in terms of in vitro bio-stimulatory activity. A commercially available bio-stimulant, ComCat®, was used as a positive control, while a glucose solution for respiration tests and distilled water for germination tests served as negative controls. Compared with the controls, significant (P < 0.05) increases in the respiration rate of monoculture yeast cells and seedling root growth in the majority of test crops after treatment with SS confirmed its in vitro bio-stimulatory potential. Seedling root growth compared favourably with results obtained with the commercial bio-stimulant. Subsequently, the in vivo yield response of selected crops to foliar treatment with SS was investigated under field conditions using ComCat® as a positive control. All trials were laid out in a complete randomised block design. Foliar applications of SS at 5 mg/L under a drip irrigation system contributed to significant (P < 0.05) yield increases in lettuce (by 20.0 t/ha) and carrots (by 22.2 t/ha), which compared favourably with the positive control, but not in beetroot and cabbage. Beetroot yield was enhanced whereas cabbage yield was decreased markedly, but not significantly, by the SS treatment, compared with the negative control. The results confirmed both the in vitro and in vivo bio-stimulatory potential of a seed suspension of L. albus cv. Betsuhana White in terms of its ability to manipulate early seedling growth and enhance the yield of selected vegetable crops.

Phytochemical Screening, In-vitro and In-vivo Anti-diabetic Activity of Nelumbo nucifera Leaves against Alloxan-Induced Diabetic Rabbits

2019 ◽  
Author(s):  
Shahid Maqbool ◽  
Najeeb Ullah ◽  
Aqal Zaman ◽  
Atif Akbar ◽  
Saima Saeed ◽  
...  
Keyword(s):  
Body Weight ◽  
Quantitative Analysis ◽  
Cold Water ◽  
Nelumbo Nucifera ◽  
Plant Leaves ◽  
Standard Drug ◽  
Antihyperglycemic Activity ◽  
Butanol Fraction

A recent survey has demonstrated that traditional healers and herbalists frequently use Nelumbo nucifera plant to treat diabetes. The plant investigated here has been previously reported as natural folk medicine, but until now no scientific investigation of potential anti-diabetic effects have been reported. Plant leaves of Nelumbo nucifera were screened out for quantitative analysis of phytochemicals, in-vitro anti-diabetic activity (Alpha-amylase inhibitory assay) and In-vivo anti-diabetic assay of animals (rabbits). Quantitative analysis indicates that different phytochemicals were present in plant leaves extract/fractions. In-vitro antidiabetic analysis results poses that butanol fraction is significantly more active than other fractions or extract in antihyperglycemic activity with minimum IC50 value of 8.56µg/ml. For In-vivo anti-diabetic assay, animals (rabbits) were examined and animals were divided into 10 groups and except blank group all were treated with standard and plant extracts/fractions doses (25mg and 50mg per Kg body weight) as an aqueous suspension. Their blood sugar level was thoroughly recorded with a glucometer before the administration of sample and standard drug. Alloxan Monohydrate was administrated orally (as an ice-cold water solution) at dose rate 150mg/kg body weight for one week to make animals hyperglycemic. In-vivo analysis indicates that butanol fraction is the most active fraction in antihyperglycemic activity. So Leaves of N. nucifera can be used as antidiabetic remedy. So, it is strongly recommended that N. nucifera leaves should be elucidated further for isolation of active phytochemicals.

Synthesis of α-amyrin derivatives and their in vivo antihyperglycemic activity☆

2009 ◽  
Vol 44 (3) ◽  
pp. 1215-1222 ◽  
Author(s):  
T. Narender ◽  
T. Khaliq ◽  
A.B. Singh ◽  
M.D. Joshi ◽  
P. Mishra ◽  
...  
Keyword(s):  
Antihyperglycemic Activity

Synthesis and in vivo antihyperglycemic activity of 5-(1H-pyrazol-3-yl)methyl-1H-tetrazoles

2005 ◽  
Vol 15 (8) ◽  
pp. 2115-2117 ◽  
Author(s):  
Ashoke Sharon ◽  
Ramendra Pratap ◽  
Priti Tiwari ◽  
Arvind Srivastava ◽  
P.R. Maulik ◽  
...  
Keyword(s):  
Antihyperglycemic Activity
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