scholarly journals Development of an amplified luminescent proximity homogeneous assay for the detection of sulfonamides in animal‐derived products

2021 ◽  
Author(s):  
Yong Jin ◽  
Yanping He ◽  
Dali Zhao ◽  
Yan Chen ◽  
Qiang Xue ◽  
...  
Keyword(s):  
Homogeneous Assay

scholarly journals High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Markus H. Kainulainen ◽  
Eric Bergeron ◽  
Payel Chatterjee ◽  
Asheley P. Chapman ◽  
Joo Lee ◽  
...  
Keyword(s):  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antibodies ◽  
Negative Results ◽  
A Value ◽  
Elisa Testing ◽  
The World ◽  
Antibody Titers ◽  
Qualitative Assay ◽  
Homogeneous Assay ◽  
Asymptomatic Infections

AbstractSARS-CoV-2 emerged in late 2019 and has since spread around the world, causing a pandemic of the respiratory disease COVID-19. Detecting antibodies against the virus is an essential tool for tracking infections and developing vaccines. Such tests, primarily utilizing the enzyme-linked immunosorbent assay (ELISA) principle, can be either qualitative (reporting positive/negative results) or quantitative (reporting a value representing the quantity of specific antibodies). Quantitation is vital for determining stability or decline of antibody titers in convalescence, efficacy of different vaccination regimens, and detection of asymptomatic infections. Quantitation typically requires two-step ELISA testing, in which samples are first screened in a qualitative assay and positive samples are subsequently analyzed as a dilution series. To overcome the throughput limitations of this approach, we developed a simpler and faster system that is highly automatable and achieves quantitation in a single-dilution screening format with sensitivity and specificity comparable to those of ELISA.

Evaluation of semi-homogeneous assay formats for dual-specificity antibodies

2013 ◽  
Vol 387 (1-2) ◽  
pp. 51-56 ◽  
Author(s):  
Guoying Jiang ◽  
Chung W. Lee ◽  
Pin Yee Wong ◽  
Hélène Gazzano-Santoro
Keyword(s):  
Dual Specificity ◽  
Homogeneous Assay

scholarly journals Improved Specificity of a New Homogeneous Assay for LDL-Cholesterol in Serum with Abnormal Lipoproteins

2006 ◽  
Vol 52 (5) ◽  
pp. 886-888 ◽  
Author(s):  
Yasumasa Iwasaki ◽  
Hiroyuki Matsuyama ◽  
Nobuo Nakashima
Keyword(s):  
Clinical Practice ◽  
Obstructive Jaundice ◽  
Ldl Cholesterol ◽  
Performance Characteristics ◽  
New Method ◽  
Assay Performance ◽  
Basic Performance ◽  
Homogeneous Method ◽  
Homogeneous Assay ◽  
Improved Performance

Abstract Background: Although a homogeneous assay for serum LDL-cholesterol (LDL-C) has become a routine clinical procedure, problems remain in assay performance characteristics. Methods: We examined the performance of a recently developed automated homogeneous assay (New-Daiichi assay) for serum LDL-C and compared the results with those obtained by the current homogeneous method (Denka-Seiken assay) or by ultracentrifugation as a control. Results: The New-Daiichi assay showed satisfactory basic performance characteristics such as reproducibility, linearity, and stability. There was no interference in the assay by various substances examined. The LDL-C values obtained with this method correlated well with those obtained by ultracentrifugation. In samples from patients with obstructive jaundice, both methods detected cholesterol from abnormal lipoproteins (such as lipoprotein-X and -Y), but the New-Daiichi assay was less reactive and more specific for LDL-C. Conclusion: The new method has improved performance for the accurate measurement of LDL-C in clinical practice.

Screening Hybridomas for Cell Surface Antigens by High-Throughput Homogeneous Assay and Flow Cytometry

2014 ◽  
pp. 81-103
Author(s):  
Alejandro Uribe-Benninghoff ◽  
Teresa Cabral ◽  
Efthalia Chronopoulou ◽  
Jody D. Berry ◽  
Cindi R. Corbett
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
High Throughput ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
Homogeneous Assay

Homogeneous assay for zearalenone analogues and their docking studies with apo-/holo-estrogen receptors

2019 ◽  
Vol 11 (2) ◽  
pp. 192-199 ◽  
Author(s):  
Jie Zhang ◽  
Wenfu Wu ◽  
Yifan Song ◽  
Ligang Hou ◽  
Tiezhu Li ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Estrogen Receptors ◽  
Docking Studies ◽  
Structural Basis ◽  
Homogeneous Assay

A homogeneous assay was developed for zearalenone analogues and the structural basis of their estrogenicity was demonstrated by molecular docking.

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