Alterations in the oxygen consumption, condition index and concentration of free amino acids inCorbicula fluminea, (mollusca: Pelecypoda) exposed to sodium dodecyl sulfate

1988 ◽  
Vol 7 (4) ◽  
pp. 301-315 ◽  
Author(s):  
Robert L. Graney ◽  
John P. Giesy
Keyword(s):  
Amino Acids ◽  
Oxygen Consumption ◽  
Sodium Dodecyl Sulfate ◽  
Free Amino Acids ◽  
Free Amino ◽  
Sodium Dodecyl ◽  
Condition Index ◽  
Dodecyl Sulfate

A two-part energy burden imposed by growth of Enterobacter cloacae and Escherichia coli in sodium dodecyl sulfate

1993 ◽  
Vol 39 (6) ◽  
pp. 555-561 ◽  
Author(s):  
Arden Aspedon ◽  
Kenneth W. Nickerson
Keyword(s):  
Oxygen Consumption ◽  
Sodium Dodecyl Sulfate ◽  
Osmotic Stress ◽  
Stationary Phase ◽  
Energy Dependence ◽  
Sodium Dodecyl ◽  
Enterobacter Cloacae ◽  
Enteric Bacteria ◽  
Potassium Accumulation ◽  
Dodecyl Sulfate

Enterobacter cloacae, like most enteric bacteria, can grow in the presence of 10% sodium dodecyl sulfate (SDS). The bacteria tolerate the detergent and do not metabolize it. In a defined glucose–salts medium the growth rate remained unchanged (G = 55 min) as the detergent concentration was increased from 0 to 10% SDS. However, growth in SDS exhibited a two-part energy dependence. In part 1, the SDS-grown cells underwent rapid lysis when they ran out of energy. Cells that had entered stationary phase owing to carbon limitation lysed, while those that had entered owing to nitrogen or phosphorus limitation did not. We attribute part 1 of the energy dependence to SDS as a detergent. In part 2, the cells grown in 5 or 10% SDS exhibited longer lag periods, potassium accumulation, decreased cell yields, and higher oxygen consumption. The higher oxygen consumption occurred during both exponential phase and nitrogen-limited stationary phase. However, the decreased cell yield and higher oxygen consumption of SDS-grown cells were mimicked by cells grown in equivalent concentrations of sucrose or polyethylene glycol. We attribute part 2 of the energy dependence to SDS as a solute. Finally, with regard to the as yet unidentified bacterial osmotic stress detector, we used the micelle-forming nature of SDS to conclude that the detector was responding to turgor pressure – water activity rather than to osmolarity itself.Key words: sodium dodecyl sulfate, bacterial detergent resistance, osmotic stress, oxygen consumption, energy-dependent cell lysis.

scholarly journals Preparation of polyribosome aminoacyl-transfer ribonucleic acid from the muscle of chick embryos.

1975 ◽  
Vol 147 (3) ◽  
pp. 473-477 ◽  
Author(s):  
M Nwagwu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Sodium Dodecyl Sulphate ◽  
Ribonucleic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Sodium Dodecyl ◽  
Specific Radioactivity ◽  
Chick Embryos ◽  
Aminoacyl Transfer

A procedure for preparing polyribosome aminoacyl-tRNA free from contamination by supernatant aminoacyl-tRNA and free amino acids is described. Important features of the procedure are the use of acidic buffers to help protect the amino acid-tRNA linkage and the inclusion of sodium dodecyl sulphate, to inhibit ribonuclease activity. The specific radioactivity of polyribosome aminoacyl-tRNA is high within 30s and reaches a maximum in 2 1/2 min, well ahead of polyribosome peptides which, as described by Herrmann et al. (1971), attain maximum specific radioactivity in about 10 min.

A transport-dependent energy burden imposed by growth of Enterobacter cloacae in the presence of 10% sodium dodecyl sulfate

1984 ◽  
Vol 30 (5) ◽  
pp. 699-702 ◽  
Author(s):  
Vance C. Kramer ◽  
Kenneth W. Nickerson
Keyword(s):  
Oxygen Consumption ◽  
Membrane Potential ◽  
Sodium Dodecyl Sulfate ◽  
Glucose Utilization ◽  
Sodium Dodecyl ◽  
Enterobacter Cloacae ◽  
Cell Yield ◽  
Sugar Alcohols ◽  
Dodecyl Sulfate ◽  
Rate Of Oxygen Consumption

Growth of Enterobacter cloacae in a glucose asparagine salts medium in the presence of 10% sodium dodecyl sulfate entailed an energy burden in the form of a 20% decreased cell yield, a 30% faster rate of glucose utilization, and a 70% increased rate of oxygen consumption. Similar detergent-induced decreases in cell yield were observed with 10 other sugars and sugar alcohols. Only glycerol supported equivalent cell growth in the presence and absence of sodium dodecyl sulfate. A model is presented which interprets these observations in terms of an altered membrane potential which makes active transport energetically less efficient.

scholarly journals Hydrolysis of Pork Muscle Sarcoplasmic Proteins byLactobacillus curvatus and Lactobacillus sake

1999 ◽  
Vol 65 (2) ◽  
pp. 578-584 ◽  
Author(s):  
Silvina Fadda ◽  
Yolanda Sanz ◽  
Graciela Vignolo ◽  
M.-Concepción Aristoy ◽  
Guillermo Oliver ◽  
...  
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Lactobacillus Curvatus ◽  
Whole Cells ◽  
Sarcoplasmic Proteins ◽  
Lactobacillus Sake ◽  
Cell Extracts

ABSTRACT Lactobacillus curvatus CECT 904 and Lactobacillus sake CECT 4808 were selected on the basis of their proteolytic activities against synthetic substrates. Further, the effects of whole cells, cell extracts, and a combination of both enzymatic sources on muscle sarcoplasmic proteins were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography analyses. Strains of both species displayed proteinase activities on five sarcoplasmic proteins. The inoculation of whole cells caused a degradation of peptides, whereas the addition of cell extracts resulted in the generation of both hydrophilic and hydrophobic peptides. This phenomenon was remarkably more pronounced when L. curvatus was involved. Whole cells also consumed a great amount of free amino acids, while the addition of intracellular enzymes contributed to their generation.L. sake accounted for a greater release of free amino acids. In general, cell viability and also proteolytic events were promoted when cell suspensions were provided with cell extracts as an extra source of enzymes.

Study of mixed micellar aqueous solutions of sodium dodecyl sulfate and amino acids

2013 ◽  
Vol 75 (4) ◽  
pp. 357-365 ◽  
Author(s):  
A. Ali ◽  
V. Bhushan ◽  
N. A. Malik ◽  
K. Behera
Keyword(s):  
Amino Acids ◽  
Sodium Dodecyl Sulfate ◽  
Aqueous Solutions ◽  
Sodium Dodecyl ◽  
Dodecyl Sulfate
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