Toxic Responses of Palladium Accumulation in Duckweed Lemna minor : Determination of Biomarkers

2021 ◽  
Author(s):  
Souleimen Jmii ◽  
David Dewez
Keyword(s):  
Lemna Minor ◽  
Toxic Responses

scholarly journals Modelagem otimizada de indicadores biológicos de qualidade da água

Revista DAE ◽  
2019 ◽  
Vol 221 (68) ◽  
pp. 131-141
Author(s):  
Gabriel da Costa Cantos Jerônimo ◽  
Luiz Felipe Ramos Turci ◽  
Paulo Augusto Zaitune Pamplin ◽  
Patrícia Neves Mendes
Keyword(s):  
Population Growth ◽  
Logistic Model ◽  
Growth Models ◽  
Lemna Minor ◽  
Growth Data ◽  
Model Quality ◽  
Nonlinear Growth ◽  
Statistical Indicators ◽  
Selection Of

Resumo 27/06/2018 DOI: https://doi.org/10.36659 /dae.2020.011 Turci, L. F. R Pamplin, P. A. Z https://orcid.org/0000-0001-7516-0963 https://orcid.org/0000-0001-7318-9121 O estudo de plantas aquáticas (macrófitas) é importante, uma vez que essas plantas apresentam potencial de utilização em estudos de ecotoxicologia, como bioindicadores no tratamento de águas residuárias. A mode- lagem criteriosa do crescimento dessas plantas, especificamente a Lemna minor, é útil na determinação das condições de otimização dessas aplicações; assim, deseja-se sempre obter o modelo que melhor represente a dinâmica de crescimento populacional da planta em estudo. Neste trabalho, apresenta-se uma metodologia de ajuste e seleção de modelos de crescimento não lineares com base em indicadores estatísticos que servem como avaliadores de qualidade dos modelos. Para ilustrar o uso da metodologia, foi feito o cultivo de Lemna minor em meio Steinberg e foram ajustados três modelos aos dados médios de crescimento de suas frondes, selecionando o modelo Logístico como o melhor. Palavras-chave: Modelo de crescimento populacional. Avaliadores de qualidade. Lemna minor. Abstract The study of aquatic plants (macrophytes) is important since such plants present a potential utilization in ecotoxi- cology as bioindicators, as well in wastewater treatment. The criterious growth modelling of such plants, specifically Lemna minor, is useful for the determination of the optimal conditions of mentionedin applications - so one always looks for the best model that represents the dynamic of population growth of the plant in study. This work presents a methodology of adjustment and selection of nonlinear growth models based on statistical indicators, which work as quality evaluators for the models. To illustrate this methodology, Lemna minor was grown in Steinberg environ- ment, and three models were fitted to the fronds growth data, the Logistic model was selected as the best model. Keywords: Population growth model. Quality evaluators. Lemna minor.

scholarly journals A new method for the measurement of protein turnover

1975 ◽  
Vol 148 (1) ◽  
pp. 119-127 ◽  
Author(s):  
T J Humphrey ◽  
D D Davies
Keyword(s):  
Amino Acids ◽  
Carbon Atom ◽  
Protein Degradation ◽  
Culture Medium ◽  
Lemna Minor ◽  
Total Soluble Protein ◽  
Short Exposure ◽  
Protein Amino Acids ◽  
A New Technique

A new technique for the determination of rate constants of protein degradation is described. By using the method, half-lives of total soluble protein of Lemna minor during growth on full culture medium and distilled water were measured. The method involves incubating Lemna on a growth medium containing 3H2O. After a short exposure (20 min) to 3H-labelled culture medium, 3H was found in soluble amino acids, especially aspartate, glutamate, glutamine and alanine. After transfer to a 3H-free medium for 30 min, 80% of the 3H originally present in soluble amino acids was lost. These results suggest that 3H enters and leaves amino acids at the α-carbon atom, a conclusion supported by the observed labelling of glutamates. The exchange of H and 3H on the α-carbon atom is catalysed by transaminases and the speed of this exchange ensures that when the 3H2O is removed, the 3H in free amino acids is rapidly lost, thereby eliminating problems connected with metabolic pools and recycling. After an exposure of 20 min to 3H-labelled medium all protein amino acids, except for arginine, were found to be radioactive. The loss of radioactivity from protein amino acids was used to measure protein degradation.

Determination of jasmonic acid in Lemna minor (L.) by liquid chromatography with fluorescence detection

2005 ◽  
Vol 383 (5) ◽  
pp. 886-893 ◽  
Author(s):  
Janja Kristl ◽  
Marjan Veber ◽  
Božidar Krajničič ◽  
Klara Orešnik ◽  
Metka Slekovec
Keyword(s):  
Liquid Chromatography ◽  
Jasmonic Acid ◽  
Fluorescence Detection ◽  
Lemna Minor

scholarly journals Influence of Triphenyllead Chloride on Biological and Model Membranes

2000 ◽  
Vol 55 (9-10) ◽  
pp. 764-769
Author(s):  
Halina Kleszczyńska ◽  
Krzysztof Bielecki ◽  
Janusz Sarapuk ◽  
Anna Dziamska ◽  
Stanislaw Przestalski
Keyword(s):  
Lipid Membranes ◽  
Lemna Minor ◽  
Model Membranes ◽  
Lipid Phase ◽  
Experimental Conditions ◽  
Inhibition Of Growth ◽  
Model Lipid Membranes ◽  
Osmotic Stability ◽  
Spirodela Oligorrhiza

Abstract The physiological and hemolytic toxicities of triphenyllead chloride (TPhL) as well as its modyfying influence on model lipid membranes were studied. The experiments allowed the determination of TPhL concentrations causing 50% inhibition of growth of Spirodela oligorrhiza, Lemna minor and Solvinia natans (EC50), 100% hemolysis of pig erythrocytes (C100) and destabilization of planar lipid membranes (CC). Also, fluidity of erythrocyte ghosts was measured by fluorescence technique and osmotic sensitivity of erythrocytes to the presence of TPhL. All parameters studied were found to be dependent on pH, of experimental solutions and the concentration of TPhL. Acidic conditions increased EC50, C100 and CC concentrations of TPhL. Fluorescence and osmotic measurements showed that osmotic stability and fluidity decreased with increasing trimethyllead concentration. A possible mechanism of TPhL toxicity is discussed. It is assumed that TPhL is interacting with the lipid phase of the models used. It is also assumed that there may exist various, ionic and nonionic, forms of TPhL as a result of its speciation under different experimental conditions. These species, due to their differentiated lipophilicity, may exert different effects on the model membranes studied.

scholarly journals Water masses surface temperatures assessment and their effect on surrounding environment

2017 ◽  
Vol 75 (12) ◽  
pp. 2916-2925 ◽  
Author(s):  
Cristina S. C. Calheiros ◽  
Eva Barreira ◽  
Nuno M. M. Ramos ◽  
Paula M. L. Castro
Keyword(s):  
Surface Temperature ◽  
Systems Analysis ◽  
Lemna Minor ◽  
Water Masses ◽  
Zantedeschia Aethiopica ◽  
Island Effect ◽  
Surrounding Environment ◽  
Surface Temperatures ◽  
A Site

The aim of this study was to evaluate the effect of water masses on the surface temperature with direct impact on the surrounding area. Three systems were used for the study: a fully vegetated system (subsurface flow constructed wetland (CW)), a lake with no vegetation and a lake partially vegetated with Lemna minor. Infrared thermography was applied for the different systems analysis, allowing the determination of the surface temperature spatial distribution. In general, the presence of plants and water in the analyzed systems contributed to lowering the surface temperatures when comparing to its surroundings. Differences up to about 22 °C were observed in the temperature between the CW canopy and the surrounding soil, and up to about 19 °C between the lake and the surrounding border. Different plant species (Canna flaccida, Canna indica and Zantedeschia aethiopica) inhabiting the CW were also compared and slightly higher average surface temperatures were observed for C. indica. The above mentioned results are relevant in terms of supporting a strategy for water systems inclusion, for example a lake or a CW, in a site as means of having influence in the surface temperature and to some extent in the heat island effect supporting a sustainable environmental management.

scholarly journals Development of Parameters for Standardization of Duckweed (Lemna Minor L.) Raw Material

2021 ◽  
Vol 10 (1) ◽  
pp. 74-81
Author(s):  
L. N. Nikiforov ◽  
S. V. Krivoshchekov ◽  
N. E. Kolomiets ◽  
T. V. Kadyrova ◽  
N. V. Isaikina ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Raw Materials ◽  
Lemna Minor ◽  
Direct Method ◽  
Biologically Active ◽  
Raw Material ◽  
Natural Habitats ◽  
Microscopic Features ◽  
Phenolcarboxylic Acids

Introduction. Lemna minor L. (duckweed) refers to the duckweed subfamily (Lemnaceae S. F. Gray) and widely distributed in ponds of Russia. Literature data confirm the possibility of harvesting significant volumes of this raw material in natural habitat and grown in aquaculture. The process of biosynthetic accumulation in duckweed fronds provides a variety of compounds with a wide spectrum of biological activity. Therefore, the use of raw materials Lemna minor L. is promising for the development of drugs and parapharmaceutical products. Thus, it is an urgent task to quantify active components of duckweed and standardize (determination of criteria for identification, quality and safety) plant material.Aim. Establish macro- and microscopic characteristics of raw materials and develop methods for the quantitative determination of the main groups of biologically active substances (BAS) for standardization of raw duckweed.Materials and methods. Samples of duckweed was collected in natural habitats of Western Siberia. Macro- and microscopic assay, HPLC, UV-spectrometry were used in research process.Results and discussion. Were established the criteria for identification of duckweed fronds by studying external (macroscopic) and microscopic features of raw material Lemna minor L. Was developed and validated the procedure of the quantitative determination of phenolcarboxylic acids in raw material Lemna minor L.Conclusion. The study of external (macroscopic) and microscopic features provided the criteria for identification of the raw material Lemna minor L. The technique for the quantitative analysis of polysaccharides using gravimetry does not need validation, because is a direct method of substance measurement. Was validated quantification method of phenolcarboxylic acids (in terms of chlorogenic acid) by criteria of linearity, repeatability, in-laboratory precision and accuracy. Was established quality criteria for identification and quantitative assay, which can be used in the draft for normative documents for medicinal plant raw material of Lemna minor L. «Duckweed fronds».

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