scholarly journals Bioengineered three‐dimensional transparent eggshell as a chicken embryo experimentation platform for biomedical research

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Noreen Ishak ◽  
Marcus Chan ◽  
Sook C. Ang ◽  
Christine Cheung ◽  
Swee‐Hin Teoh
Keyword(s):  
Biomedical Research ◽  
Chicken Embryo ◽  
Three Dimensional ◽  
Embryo Experimentation

Applications of Scanning Microscopy in Biomedical Research

1968 ◽  
Vol 26 ◽  
pp. 354-355
Author(s):  
T. L. Hayes
Keyword(s):  
Information Content ◽  
Biomedical Research ◽  
Biomedical Applications ◽  
Three Dimensional ◽  
Dental Enamel ◽  
Resolving Power ◽  
Whole Mount ◽  
Two Parameters ◽  
Content Information ◽  
Sectioned Tissue

Biomedical applications of the scanning electron microscope (SEM) have increased in number quite rapidly over the last several years. Studies have been made of cells, whole mount tissue, sectioned tissue, particles, human chromosomes, microorganisms, dental enamel and skeletal material. Many of the advantages of using this instrument for such investigations come from its ability to produce images that are high in information content. Information about the chemical make-up of the specimen, its electrical properties and its three dimensional architecture all may be represented in such images. Since the biological system is distinctive in its chemistry and often spatially scaled to the resolving power of the SEM, these images are particularly useful in biomedical research.In any form of microscopy there are two parameters that together determine the usefulness of the image. One parameter is the size of the volume being studied or resolving power of the instrument and the other is the amount of information about this volume that is displayed in the image. Both parameters are important in describing the performance of a microscope. The light microscope image, for example, is rich in information content (chemical, spatial, living specimen, etc.) but is very limited in resolving power.

scholarly journals EZ Clear for simple, rapid, and robust mouse whole organ clearing

2022 ◽  
Author(s):  
Chih-Wei Hsu ◽  
Juan Cerda ◽  
Jason M Kirk ◽  
Williamson D. Turner ◽  
Tara L. Rasmussen ◽  
...  
Keyword(s):  
Biomedical Research ◽  
Room Temperature ◽  
Adult Mouse ◽  
Dimensional Space ◽  
Three Dimensional ◽  
Fluorescence Signal ◽  
Tissue Architecture ◽  
Tissue Clearing ◽  
Clearing Method ◽  
Three Dimensional Space

Tissue clearing for whole organ cell profiling has revolutionized biology and imaging for exploration of organs in three-dimensional space without compromising tissue architecture. But complicated, laborious procedures, or expensive equipment, as well as the use of hazardous, organic solvents prevents the widespread adoption of these methods. Here we report a simple and rapid tissue clearing method, EZ Clear, that can clear whole adult mouse organs in 48 hours in just three simple steps. Samples stay at room temperature and remain hydrated throughout the clearing process, preserving endogenous and synthetic fluorescence, without altering sample size. After wholemount clearing and imaging, EZ Cleared samples can be further processed for downstream embedding and cryosectioning followed by standard histology or immunostaining, without loss of endogenous or synthetic fluorescence signal. Overall, the simplicity, speed, and flexibility of EZ Clear make it easy to adopt and apply to diverse approaches in biomedical research.

scholarly journals Experimental and Data Analysis Considerations for Three-Dimensional Mass Spectrometry Imaging in Biomedical Research

2020 ◽  
Author(s):  
D. R. N. Vos ◽  
S. R. Ellis ◽  
B. Balluff ◽  
R. M. A. Heeren
Keyword(s):  
Mass Spectrometry ◽  
Data Analysis ◽  
Biomedical Research ◽  
Mass Spectrometry Imaging ◽  
Three Dimensional ◽  
Sample Volume ◽  
Biological Processes ◽  
Full Sample ◽  
Single Slice ◽  
Molecular Complexity

Abstract Mass spectrometry imaging (MSI) enables the visualization of molecular distributions on complex surfaces. It has been extensively used in the field of biomedical research to investigate healthy and diseased tissues. Most of the MSI studies are conducted in a 2D fashion where only a single slice of the full sample volume is investigated. However, biological processes occur within a tissue volume and would ideally be investigated as a whole to gain a more comprehensive understanding of the spatial and molecular complexity of biological samples such as tissues and cells. Mass spectrometry imaging has therefore been expanded to the 3D realm whereby molecular distributions within a 3D sample can be visualized. The benefit of investigating volumetric data has led to a quick rise in the application of single-sample 3D-MSI investigations. Several experimental and data analysis aspects need to be considered to perform successful 3D-MSI studies. In this review, we discuss these aspects as well as ongoing developments that enable 3D-MSI to be routinely applied to multi-sample studies.

scholarly journals Anatomical Structures, Cell Types, and Biomarkers Tables Plus 3D Reference Organs in Support of a Human Reference Atlas

2021 ◽  
Author(s):  
Katy Borner ◽  
Sarah A. Teichmann ◽  
Ellen M. Quardokus ◽  
James Gee ◽  
Kristen Browne ◽  
...  
Keyword(s):  
Biomedical Research ◽  
User Interfaces ◽  
Human Tissue ◽  
Three Dimensional ◽  
Cell Types ◽  
Use Cases ◽  
Anatomical Structures

This paper reviews efforts across 16 international consortia to construct human anatomical structures, cell types, and biomarkers (ASCT+B) tables and three-dimensional reference organs in support of a Human Reference Atlas. We detail the ontological descriptions and spatial three-dimensional anatomical representations together with user interfaces that support the registration and exploration of human tissue data. Four use cases are presented to demonstrate the utility of ASCT+B tables for advancing biomedical research and improving health.

scholarly journals 3D Cell Technology in Biomedical Research

2020 ◽  
Vol 44 (3) ◽  
pp. 171-174
Author(s):  
Katarina Mišković Špoljarić ◽  
Marijana Jukić ◽  
Teuta Opačak-Bernardi ◽  
Ljubica Glavaš-Obrovac
Keyword(s):  
Cell Culture ◽  
Biomedical Research ◽  
Monolayer Culture ◽  
Magnetic Levitation ◽  
Functional Model ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
3D Models ◽  
Cell Culture Techniques ◽  
Culture Techniques

Traditional two dimensional cell culture has enabled great strides in biomedicine but needs to be improved to be able to keep up with the demands of modern biomedical research. 2D monolayer culture cannot replicate tissue responses and needs to be supplemented with extensive animal research. Growing cells in three dimensional scaffolds provides a more functional model for biomedical research than traditional monolayer culture. Depending on the needs and the complexity of the model there are several ways that 3D models can be initiated. Simple spheroids can be grown in low adherence plates and in hanging drops while larger spheroids and co-cultured ones need to be grown in systems with greater support such as hydro gels. The system that offers the greatest flexibility is the magnetic levitation approach. In the paper we offer a brief resume to various 3D methods and their characteristics to ease the choice of methods for implementing 3D cell culture techniques.

scholarly journals Global Trends in Application of Stereology as a Quantitative Tool in Biomedical Research

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Maulilio J. Kipanyula ◽  
Alfred S. Sife
Keyword(s):  
Biomedical Research ◽  
Comparative Method ◽  
Three Dimensional ◽  
Great Majority ◽  
Prolific Author ◽  
Global Trends ◽  
Morphological Studies ◽  
Geometrical Features ◽  
Dimensional Parameters ◽  
Scientometric Study

Stereology is a quantitative and comparative method that utilizes planes, lines, and points for the estimation of three-dimensional parameters in morphological studies. It primarily focuses on geometrical features of objects such as number, density, length, area, and volume. A scientometric study was conducted to analyze global research trends in application of stereology in biomedical research. Stereology has gained wide application resulting into design-based stereological methods. Data for this study were retrieved from the SCOPUS database. At least 5,732 publications employing stereology as analytical tool were produced in a period of 50 years between 1966 and 2016. Half (2,858; 49.87%) of these publications were produced in the last 12 years from 2005 to 2016. The relative growth rate (RGR) of publications decreased from 1967 (0.69) to 2016 (0.03) whereas the doubling time (DT) increased from 1.00 to 20.56 in the same period. A great majority (5,332; 93.02%) of the publications retrieved from SCOPUS were journal articles in various biomedical fields. The Journal of Microscopy tops the list of journals with at least 205 articles. The most productive country was USA with at least 1663 (23.10%) publications and Aarhus Universitet tops the list of institutions with at least 306 publications. J.R. Nyengaard was the most prolific author who contributed at least 125 publications. The highly cited article had a total of 2,054 citations with an average of over 82 citations per year. Given the growing importance of stereology in biomedical research, it is necessary to promote its application among scholars.

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