Nick translation—a new assay for monitoring dna damage and repair in cultured human fibroblasts

1985 ◽  
Vol 7 (3) ◽  
pp. 267-279 ◽  
Author(s):  
Ronald D. Snyder ◽  
Dale W. Matheson
Keyword(s):  
Dna Damage ◽  
Human Fibroblasts ◽  
Nick Translation ◽  
Dna Damage And Repair ◽  
Cultured Human Fibroblasts

Zinc protects against ultraviolet A1-induced DNA damage and apoptosis in cultured human fibroblasts

1999 ◽  
Vol 69 (3) ◽  
pp. 177-190 ◽  
Author(s):  
Marie-Th’erèse Leccia ◽  
Marie-Jeanne Richard ◽  
Alain Favier ◽  
Jean-Claude B’Eani
Keyword(s):  
Dna Damage ◽  
Human Fibroblasts ◽  
Cultured Human Fibroblasts

Protection from solar simulated radiation-induced DNA damage in cultured human fibroblasts by three commercially available sunscreens

2003 ◽  
Vol 81 (7) ◽  
pp. 690-695 ◽  
Author(s):  
Pascale Reinhardt ◽  
Michelle Cybulski ◽  
James P McNamee ◽  
Jack R McLean ◽  
Wayne Gorman ◽  
...  
Keyword(s):  
Dna Damage ◽  
Solar Radiation ◽  
Comet Assay ◽  
Human Fibroblasts ◽  
Skin Aging ◽  
Cellular Level ◽  
Target Cells ◽  
Functional Changes ◽  
Cultured Human Fibroblasts ◽  
Radiation Induced

Exposure to solar radiation can produce both acute and chronic changes in the skin, including sunburn, edema, immunosuppression, premature skin aging, and skin cancer. At the cellular level, solar radiation can produce adverse structural and functional changes in membrane proteins and lipids and in chromosomal and mitochondrial DNA. The increasing awareness of these adverse effects has led the public to demand better photoprotection. In this study, the alkaline comet assay was used to evaluate the photoprotective effects of three commercially available sunscreens at sun protection factors (SPF) 15 and 30. Human fibroblasts were used as target cells to conveniently study the effects of solar simulated radiation on DNA damage in the presence and absence of sunscreens. When human fibroblasts were exposed to various doses of solar simulated radiation, DNA damage, as measured in sunscreen-protected cells by the comet assay, was not significantly different from that detected in unexposed cells. At 1.0 and 1.5 minimal erythemal doses (MED), all sunscreens, at both SPF 15 and 30, provided nearly 100% photoprotection to the fibroblasts. Further studies are required to elucidate the role of UVA in the production and repair of DNA damage in cells exposed to sunlight.Key words: sunscreen, UVA, UVB, solar simulated radiation, comet assay.

DNA Damage and Repair in the Brain After Cerebral Ischemia

2001 ◽  
Vol 1 (6) ◽  
pp. 483-495 ◽  
Author(s):  
Bentham Science Publisher Philip K. Liu
Keyword(s):  
Dna Damage ◽  
Cerebral Ischemia ◽  
Dna Damage And Repair ◽  
The Brain

scholarly journals Influence of chlorate on proteoglycan biosynthesis by cultured human fibroblasts.

1988 ◽  
Vol 263 (26) ◽  
pp. 12886-12892 ◽  
Author(s):  
H Greve ◽  
Z Cully ◽  
P Blumberg ◽  
H Kresse
Keyword(s):  
Human Fibroblasts ◽  
Cultured Human Fibroblasts

scholarly journals Fatty alcohol metabolism in cultured human fibroblasts. Evidence for a fatty alcohol cycle.

1987 ◽  
Vol 262 (36) ◽  
pp. 17412-17419 ◽  
Author(s):  
W B Rizzo ◽  
D A Craft ◽  
A L Dammann ◽  
M W Phillips
Keyword(s):  
Fatty Alcohol ◽  
Human Fibroblasts ◽  
Alcohol Metabolism ◽  
Cultured Human Fibroblasts

L-iduronidase in cultured human fibroblasts and liver

1971 ◽  
Vol 42 (2) ◽  
pp. 340-345 ◽  
Author(s):  
Reuben Matalon ◽  
J.A. Cifonelli ◽  
Albert Dorfman
Keyword(s):  
Human Fibroblasts ◽  
Cultured Human Fibroblasts
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