Cytogenetic effects of busulfan in vivo on bone marrow cells and oocytes of adult mice and liver cells of transplacentally exposed embryos

1979 ◽  
Vol 1 (3) ◽  
pp. 233-238 ◽  
Author(s):  
A. Basler ◽  
I. Theiss ◽  
G. Röhrborn
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Liver Cells ◽  
Adult Mice ◽  
Cytogenetic Effects ◽  
Marrow Cells

scholarly journals In vivo genotoxicity of treated water containing the cylindrospermopsin-producer Cylindrospermopsis raciborskii

2014 ◽  
Vol 12 (3) ◽  
pp. 474-483 ◽  
Author(s):  
A. L. Fonseca ◽  
J. Da Silva ◽  
E. A. Nunes ◽  
S. M. F. O. Azevedo ◽  
R. M. Soares
Keyword(s):  
Bone Marrow ◽  
Comet Assay ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Liver Cells ◽  
Cylindrospermopsis Raciborskii ◽  
Producer Strain ◽  
Treated Water ◽  
Marrow Cells

Cylindrospermopsin (CYN) is an alkaloid commonly produced by some cyanobacteria that has been implicated in outbreaks of human illness. The aim of this study was to investigate the genotoxicity of Cylindrospermopsis raciborskii cellular content (including CYN) and its byproducts resulting from chlorination during water treatment. DNA damage in blood and liver cells was analysed by the comet assay and micronucleus test (MN). Mice were injected intraperitoneally with the following treatments: (a) physiological saline, (b) treated water, (c) treated water plus C. raciborskii extract (CYN producer strain, CYPO-011 K), (d) C. raciborskii extract (CYN producer strain, CYPO-011 K), (e) C. raciborskii extract (CYN non producer strain), and (f) treated water plus C. raciborskii extract (CYN non producer strain) extract. After 48 h, samples were taken to perform tests (blood and liver cells to the comet assay and bone marrow to MN test). The CYPO-011 K had a genotoxic and mutagenic effects on liver and bone marrow cells. The group that received chlorine-treated water plus CYPO-011 K also exhibited genotoxic effects in the liver, as well as in the blood, and a mutagenic effect in blood marrow cells. The results emphasise the need of improving CYN monitoring in waters bodies in order to reduce the risk of human exposure.

scholarly journals Mouse fetal liver cells lack functional amphotropic retroviral receptors

Blood ◽  
1994 ◽  
Vol 84 (2) ◽  
pp. 433-439 ◽  
Author(s):  
C Richardson ◽  
M Ward ◽  
S Podda ◽  
A Bank
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Fetal Liver ◽  
Hematopoietic Cells ◽  
Retroviral Vectors ◽  
Liver Cells ◽  
Mouse Bone Marrow ◽  
Adult Mice ◽  
Fetal Liver Cells ◽  
Marrow Cells

Abstract We have been transducing mouse hematopoietic cells with the human MDR1 (MDR) gene in retroviral vectors to determine the optimal conditions for retroviral gene transfer as a model system for potential human gene therapy. In these studies, we have demonstrated transduction and expression of the human MDR gene using ecotropic and amphotropic MDR- retroviral producer lines. To obtain more mouse hematopoietic cells for detailed study, mouse fetal liver cells (FLC) have been used for MDR transduction and expression, and to reconstitute the ablated marrows of live adult mice. FLC contain hematopoietic cells that have a reconstituting capacity comparable to that of adult mouse bone marrow cells. However, to our surprise, FLC can only be transduced with ecotropic retrovirus and not with amphotropic virus. This restriction of transduction of FLC cannot be overcome by higher titer virus. The resistance to amphotropic transduction by FLC may be part of a changing developmental program that results in a different antigen repertoire on FLC as compared with adult bone marrow cells.

scholarly journals Mouse fetal liver cells lack functional amphotropic retroviral receptors

Blood ◽  
1994 ◽  
Vol 84 (2) ◽  
pp. 433-439
Author(s):  
C Richardson ◽  
M Ward ◽  
S Podda ◽  
A Bank
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Fetal Liver ◽  
Hematopoietic Cells ◽  
Retroviral Vectors ◽  
Liver Cells ◽  
Mouse Bone Marrow ◽  
Adult Mice ◽  
Fetal Liver Cells ◽  
Marrow Cells

We have been transducing mouse hematopoietic cells with the human MDR1 (MDR) gene in retroviral vectors to determine the optimal conditions for retroviral gene transfer as a model system for potential human gene therapy. In these studies, we have demonstrated transduction and expression of the human MDR gene using ecotropic and amphotropic MDR- retroviral producer lines. To obtain more mouse hematopoietic cells for detailed study, mouse fetal liver cells (FLC) have been used for MDR transduction and expression, and to reconstitute the ablated marrows of live adult mice. FLC contain hematopoietic cells that have a reconstituting capacity comparable to that of adult mouse bone marrow cells. However, to our surprise, FLC can only be transduced with ecotropic retrovirus and not with amphotropic virus. This restriction of transduction of FLC cannot be overcome by higher titer virus. The resistance to amphotropic transduction by FLC may be part of a changing developmental program that results in a different antigen repertoire on FLC as compared with adult bone marrow cells.

Acute cytogenetic effects of potassium bromate on rat bone marrow cells in vivo

1988 ◽  
Vol 206 (4) ◽  
pp. 455-458 ◽  
Author(s):  
Kimiko Fujie ◽  
Hajime Shimazu ◽  
Mitsuko Matsuda ◽  
Taketoshi Sugiyama
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Potassium Bromate ◽  
Cytogenetic Effects ◽  
Rat Bone ◽  
Marrow Cells

scholarly journals Methylmercury-induced Cytogenetic Damage in Syrian Hamster Bone Marrow Cells

1984 ◽  
Vol 3 (4) ◽  
pp. 295-301
Author(s):  
J. B. Mailhes
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Single Injection ◽  
Cytogenetic Damage ◽  
Cytogenetic Effects ◽  
Response Increase ◽  
Environmental Toxin ◽  
Positive Controls ◽  
Marrow Cells

Methylmercury (MeHg) is an environmental toxin capable of interacting with chromatin and suspected of inducing chromosomal damage. In order to further evaluate the cytogenetic effects of MeHg in vivo, Syrian hamsters received a single injection of either 5, 10, 15, or 20 mg/kg MeHg 24 hours presacrifice and preparation of bone marrow cells for cytogenetic analysis. Negative and positive controls (0.25 mg/kg Trenimon) were also incorporated into the experimental design. The most prevalant type of cytogenetic damage observed was chromosome pulverization. A dose-response increase in the incidence of chromosomal aberrations following MeHg treatment was not observed. However, both MeHg- and Trenimon-treated hamsters had significantly higher proportions of cells with pulverized chromosomes than did controls. The observation that fewer cells with pulverized chromosomes were detected in the 15 and 20 mg/kg MeHg groups as compared with the 5 and 10 mg/kg MeHg groups was attributed to cellular toxicity. These data indicate that MeHg severely damages hamster bone marrow chromosomes, as demonstrated by chromosome pulverization.

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