Genomics-based food-borne pathogen testing and diagnostics: Possibilities for the U.S. department of agriculture's food safety and inspection service

2007 ◽  
Vol 48 (5) ◽  
pp. 363-368 ◽  
Author(s):  
James Withee ◽  
Kerry L. Dearfield
Keyword(s):  
Food Safety ◽  
Food Borne ◽  
Pathogen Testing ◽  
Inspection Service ◽  
The U.S

Salmonella Enteritidis in Meat, Poultry, and Pasteurized Egg Products Regulated by the U.S. Food Safety and Inspection Service, 1998 through 2003

2007 ◽  
Vol 70 (3) ◽  
pp. 582-591 ◽  
Author(s):  
PATRICIA L. WHITE ◽  
ALECIA L. NAUGLE ◽  
CHARLENE R. JACKSON ◽  
PAULA J. FEDORKA-CRAY ◽  
BONNIE E. ROSE ◽  
...  
Keyword(s):  
Food Safety ◽  
Salmonella Enteritidis ◽  
Phage Type ◽  
Control Point ◽  
Electrophoretic Pattern ◽  
Electrophoretic Analysis ◽  
Critical Control Point ◽  
Egg Products ◽  
Inspection Service ◽  
The U.S

The U.S. Food Safety and Inspection Service (FSIS) tests for Salmonella in meat, poultry, and egg products through three regulatory testing programs: the Pathogen Reduction–Hazard Analysis and Critical Control Point (PR-HACCP) program, the ready-to-eat program for meat and poultry products, and the pasteurized egg products program. From 1998 through 2003, 293,938 samples collected for these testing programs were analyzed for the presence of Salmonella enterica serotypes. Of these samples, 12,699 (4.3%) were positive for Salmonella, and 167 (1.3%) of the positive samples (0.06% of all samples) contained Salmonella Enteritidis. The highest incidence of Salmonella Enteritidis was observed in ground chicken PR-HACCP samples (8 of 1,722 samples, 0.46%), and the lowest was found in steer-heifer PR-HACCP samples (0 of 12,835 samples). Salmonella Enteritidis isolates were characterized by phage type, pulsed-field gel electrophoretic pattern, and antimicrobial susceptibility. Phage typing of 94 Salmonella Enteritidis isolates identified PT13 (39 isolates) and PT8 (36 isolates) as the most common types. One isolate from a ready-to-eat ham product was characterized as PT4. Electrophoretic analysis of 148 Salmonella Enteritidis isolates indicated genetic diversity among the isolates, with 28 unique XbaI electrophoretic patterns identified. Of these 148 isolates, 136 (92%) were susceptible to each of 16 antimicrobials tested. Two isolates were resistant to ampicillin alone, and 10 isolates were resistant to two or more antimicrobials. Isolation of Salmonella Enteritidis from FSIS-regulated products emphasizes the need for continued consumer education on proper food handling and cooking practices and continued work to decrease the prevalence of Salmonella in meat, poultry, and pasteurized egg products.

scholarly journals Cannabis microbiome sequencing reveals several mycotoxic fungi native to dispensary grade Cannabis flowers

F1000Research ◽  
2016 ◽  
Vol 4 ◽  
pp. 1422 ◽  
Author(s):  
Kevin McKernan ◽  
Jessica Spangler ◽  
Lei Zhang ◽  
Vasisht Tadigotla ◽  
Yvonne Helbert ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Food Safety ◽  
Agricultural Products ◽  
Third Party ◽  
Fungal Communities ◽  
Regulatory Requirement ◽  
Safety Testing ◽  
Food Borne ◽  
Generation Sequencing ◽  
The U.S

The Center for Disease Control estimates 128,000 people in the U.S. are hospitalized annually due to food borne illnesses. This has created a demand for food safety testing targeting the detection of pathogenic mold and bacteria on agricultural products. This risk extends to medicalCannabisand is of particular concern with inhaled, vaporized and even concentratedCannabisproducts.As a result, third party microbial testing has become a regulatory requirement in the medical and recreationalCannabismarkets, yet knowledge of theCannabismicrobiome is limited. Here we describe the first next generation sequencing survey of the fungal communities found in dispensary basedCannabisflowers by ITS2 sequencing, and demonstrate the sensitive detection of several toxigenicPenicilliumandAspergillusspecies, includingP. citrinum and P. paxilli,that were not detected by one or more culture-based methods currently in use for safety testing.

Determination of Pentachlorophenol in Animal Tissues: A Canadian Perspective

1990 ◽  
Vol 73 (6) ◽  
pp. 838-841
Author(s):  
James D Macneil ◽  
John R Patterson ◽  
Adrian C Fesser ◽  
Valerie K Martz
Keyword(s):  
Food Safety ◽  
Animal Tissue ◽  
Animal Tissues ◽  
Department Of Agriculture ◽  
National Surveys ◽  
Relative Standard ◽  
Canadian Perspective ◽  
Inspection Service ◽  
The U.S

Abstract Analytical methods for pentachlorophenol (PCP) residues In edible animal tissue have been reviewed, with particular reference to gas chromatographic methods of analysis. Results of analyses demonstrate that significant residues of PCP can persist for several weeks In animals exposed to contaminated bedding. National surveys In Canada have found that the incidence of PCP residues In pork in excess of 0.1 ppm was reduced from 32% of survey samples In 1981- 1982 to 6.6% of samples tested In 1987-1988. An Interlaboratory sample exchange among Canadian laboratories demonstrated that the PCP analytical method currently used by Agriculture Canada could be successfully transferred to other laboratories. An exchange of samples between regulatory laboratories of Agriculture Canada and the Food Safety and Inspection Service of the U.S. Department of Agriculture (USDA) demonstrated equivalency of results for the 2 methods currently used in the respective laboratories, with relative standard deviations for analytical results ranging from 4.4 to 22.2%.

Assessing the Impact of Retailer Disclosure on the Effectiveness of U.S. Meat and Poultry Recalls

2020 ◽  
Vol 83 (12) ◽  
pp. 2095-2101
Author(s):  
JIANBIN YU ◽  
NEAL H. HOOKER
Keyword(s):  
Food Safety ◽  
Plant Size ◽  
Policy Debate ◽  
Recovery Rates ◽  
Disclosure Policy ◽  
Poultry Products ◽  
Inspection Service ◽  
The Impact ◽  
The U.S ◽  
Lower Recovery

ABSTRACT In August 2008, the Food Safety and Inspection Service of the U.S. Department of Agriculture (USDA) launched a new policy that required publication of a list of retail consignees for the meat and poultry products part of class I recalls, those with the greatest potential impact on public health. In this study, two recall effectiveness measures (recovery rate and completion time) and a difference-in-difference method were used to examine the effects of retailer disclosures. When controlling for factors previously determined to impact recall effectiveness, including product type, reasons for recall, the amount of food recalled, plant size, and the way the problem was discovered, no significant impact on recall effectiveness was discerned under the current disclosure policy. Recalls for bacterial contamination had higher recovery rates. Larger recalls had lower recovery rates and longer completion times. Recalls issued by very small plants had lower recovery rates. Compared with other stakeholders, government agency discovery of the problem was associated with lower recovery rates. As the U.S. Food and Drug Administration considers a similar retailer disclosure policy for foods regulated under the Food Safety Modernization Act, such lessons from the USDA experience should inform the policy debate. HIGHLIGHTS

scholarly journals Cannabis microbiome sequencing reveals several mycotoxic fungi native to dispensary grade Cannabis flowers

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 1422 ◽  
Author(s):  
Kevin McKernan ◽  
Jessica Spangler ◽  
Lei Zhang ◽  
Vasisht Tadigotla ◽  
Yvonne Helbert ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Food Safety ◽  
Food Industry ◽  
Agricultural Products ◽  
Third Party ◽  
Regulatory Requirement ◽  
Safety Testing ◽  
Food Borne ◽  
Generation Sequencing ◽  
The U.S

The Center for Disease Control estimates 128,000 people in the U.S. are hospitalized annually due to food borne illnesses. This has created a demand for food safety testing targeting the detection of pathogenic mold and bacteria on agricultural products. This risk extends to medicalCannabisand is of particular concern with inhaled, vaporized and even concentratedCannabisproducts.As a result, third party microbial testing has become a regulatory requirement in the medical and recreationalCannabismarkets, yet knowledge of theCannabismicrobiome is limited. Here we describe the first next generation sequencing survey of the microbial communities found in dispensary basedCannabisflowers and demonstrate the limitations in the culture-based regulations that are being superimposed from the food industry.

Investigating the Suitability of Semicarbazide as an Indicator of Preharvest Nitrofurazone Use in Raw Chicken

2020 ◽  
Vol 83 (8) ◽  
pp. 1368-1373
Author(s):  
JOHN JOHNSTON ◽  
RANDOLPH DUVERNA ◽  
MICHAEL WILLIAMS ◽  
RITA KISHORE ◽  
CATALINA YEE ◽  
...  
Keyword(s):  
Food Safety ◽  
Study Design ◽  
Contact Time ◽  
Frozen Storage ◽  
Trading Partner ◽  
Department Of Agriculture ◽  
Antimicrobial Interventions ◽  
Inspection Service ◽  
Processing Steps ◽  
The U.S

ABSTRACT Semicarbazide (SEM) is the U.S. Food and Drug Administration's official marker for nitrofurazone use in food animals. The U.S. Department of Agriculture Food Safety and Inspection Service conducted a study to evaluate the source of SEM that was identified by a U.S. trading partner in a subset of chicken samples presented for inspection, even though nitrofurazone has been banned from use in U.S. food-producing animals since 2002. The study design included analyses to detect and quantify total and bound SEM in chicken collected from the eight U.S. establishments that were associated with the reported detection of SEM. Samples were collected immediately following evisceration, chilling, and cutting carcass into parts (cut-up). Although antimicrobial interventions (processes to reduce pathogen concentrations) are typically used at all three of these processing steps, the product contact time during chilling is significantly longer (hours versus seconds) than during evisceration and cut-up. In addition, parts were analyzed after 0, 10, 20, and 30 days of frozen storage. No postevisceration samples tested positive for SEM; however, most samples collected postchilling and after cut-up tested positive. The absence of SEM in postevisceration samples and detection in the subsequent postchilling samples and after the cut-up samples suggest that the detection of SEM in the sampled products is not indicative of preharvest nitrofurazone use and may be a result of postharvest processing in these establishments. HIGHLIGHTS

Foodborne Outbreaks Reported to the U.S. Food Safety and Inspection Service, Fiscal Years 2007 through 2012

2016 ◽  
Vol 79 (3) ◽  
pp. 442-447 ◽  
Author(s):  
KIS ROBERTSON ◽  
ALICE GREEN ◽  
LATASHA ALLEN ◽  
TIMOTHY IHRY ◽  
PATRICIA WHITE ◽  
...  
Keyword(s):  
Food Safety ◽  
Federal State ◽  
Chi Square ◽  
Local Public Health ◽  
Poultry Products ◽  
Chi Square Test ◽  
State And Local ◽  
Inspection Service ◽  
Insight Into ◽  
The U.S

ABSTRACT The U.S. Department of Agriculture's Food Safety and Inspection Service (FSIS) works closely with federal, state, and local public health partners to investigate foodborne illness outbreaks associated with its regulated products. To provide insight into outbreaks associated with meat and poultry, outbreaks reported to FSIS during fiscal years 2007 through 2012 were evaluated. Outbreaks were classified according to the strength of evidence linking them to an FSIS-regulated product and by their epidemiological, etiological, and vehicle characteristics. Differences in outbreak characteristics between the period 2007 through 2009 and the period 2010 through 2012 were assessed using a chi-square test or Mann-Whitney U test. Of the 163 reported outbreaks eligible for analysis, 89 (55%) were identified as possibly linked to FSIS-regulated products and 74 (45%) were definitively linked to FSIS-regulated products. Overall, these outbreaks were associated with 4,132 illnesses, 772 hospitalizations, and 19 deaths. Shiga toxin–producing Escherichia coli was associated with the greatest proportion of reported outbreaks (55%), followed by Salmonella enterica (34%) and Listeria monocytogenes (7%). Meat and poultry products commercially sold as raw were linked to 125 (77%) outbreaks, and of these, 105 (80%) involved beef. Over the study period, the number of reported outbreaks definitively linked to FSIS-regulated products (P =0.03) declined, while the proportion of culture-confirmed cases (P = 0.0001) increased. Our findings provide insight into the characteristics of outbreaks associated with meat and poultry products.

Comparison of Recoveries of Selected Organochlorine Pesticides, Related Compounds, and Polychlormated Biphenyls 1254 and 1260 from Fat after Kuderna-Danish and TurboVap Concentration

1995 ◽  
Vol 78 (5) ◽  
pp. 1294-1302
Author(s):  
Helen T McCarthy ◽  
Anna R Sullivan
Keyword(s):  
Gas Chromatography ◽  
Food Safety ◽  
Turnaround Time ◽  
Electron Capture Detection ◽  
Precision Data ◽  
Significant Difference ◽  
Poultry Fat ◽  
Inspection Service ◽  
Related Compounds ◽  
The U.S

Abstract A 2-part study was performed to compare tho Tur-boVap evaporator v/ith the traditional Kuderna-Darv-Ish concentrator for analysis of selected pesticides and related compounds (study 1) and polychlorinated biphenyls (PCBs) (study 2) In fat samples. To compare results, bias and precision data were evaluated for pesticides and related compounds and bias v/as evaluated for PCBs. For study 1, 20 samples of poultry fat were spiked with known amounts of 13 pesticides and related compounds. Samples were extracted v/ith petroleum ether, cleaned up with Florisil, and evaporated in either a Kudorna-Danlsh concentrator or a TurboVap evaporator. The pesticides and related compounds were identified and quantitated by gas chromatography v/ith electron capture detection. Biases, as indicated by average recoveries of pesticides and related compounds, were 63 and 73%, respectively, for the TurboVap and Kudema-Danlsh procedures. Recoveries of pesticides and related compounds, except methoxychlor, by tho TurboVap evaporator were significantly lower (p ≤ 0.05) than recoveries by Kudema-Danish concentration. Average recoveries with the TurboVap procedure were 10% lower than recoveries with the Kudema-Danlsh procedure. Precisions for both procedures were determined by testing for equality of variances with the F-test. A significant difference (p ≤ 0.05) for precision between the 2 procedures was found only for endrin. Study 2 was conducted on 14 accreditation PCB samples from the U.S. Department of Agriculture’s Food Safety and Inspection Service. PCB recoveries were 70–125% with the Kuderna-Danish apparatus and 69–106% with the TurboVap evaporator. No significant difference (p > 0.05) was found between the 2 procedures for PCB analysis. The TurboVap ovaporator gave consistently lower results for pesticides and related compounds compared with the traditional Kuderna-Danish concentrator; precisions for both procedures were similar. Results for PCBs analyzed by both procedures v/cre not significantly different. Lower recoveries for pcstlcidos and rotated compounds notwithstanding. tho TurboVap concentrator offers advantages over tho older Kudorna-Danlsh ovaporator In terms of turnaround time and efficient use of laboratory space.

Incidence of Listeria spp. in Ready-to-Eat Food Processing Plant Environments Regulated by the U.S. Food Safety and Inspection Service and the U.S. Food and Drug Administration

2018 ◽  
Vol 81 (7) ◽  
pp. 1063-1067 ◽  
Author(s):  
ROBERT G. REINHARD ◽  
ROBIN M. KALINOWSKI ◽  
PETER W. BODNARUK ◽  
JOSEPH D. EIFERT ◽  
RENEE R. BOYER ◽  
...  
Keyword(s):  
Food Safety ◽  
Drug Administration ◽  
Food Processing ◽  
Food And Drug Administration ◽  
The United States ◽  
Food Plants ◽  
Production Environment ◽  
Positive Rate ◽  
Inspection Service ◽  
The U.S

ABSTRACT A multiyear survey of 31 ready-to-eat (RTE) food processing plants in the United States was conducted to determine the incidence of Listeria spp. in various RTE production environments. Samples were collected from 22 RTE plants regulated by the U.S. Department of Agriculture's Food Safety and Inspection Service (FSIS) and from 9 RTE food plants regulated by the U.S. Department of Health and Human Services' Food and Drug Administration (FDA). Only nonfood contact surfaces in the RTE manufacturing areas with exposed RTE product were sampled. Each sample was individually analyzed for the presence of Listeria spp. by using a PCR-based rapid assay. In total, 4,829 samples were collected from various locations, including freezers, equipment framework, floors, walls, wall-floor junctures, drains, floor mats, doors, and cleaning tools. Nine (29%) of the facilities had zero samples positive for Listeria spp. in the production environment, whereas 22 (71%) had one or more samples positive for Listeria spp. The total incidence of Listeria spp. in all RTE food plants was 4.5%. The positive rate in plants regulated by the FSIS ranged from 0 to 9.7%, whereas the positive rate in plants regulated by the FDA ranged from 1.2 to 36%.

Sign in / Sign up

Export Citation Format

Share Document