scholarly journals Enzymatic hydrolysis of lignocellulosic biomass from low to high solids loading

2016 ◽  
Vol 17 (5) ◽  
pp. 489-499 ◽  
Author(s):  
Hong-Zhang Chen ◽  
Zhi-Hua Liu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
High Solids ◽  
Solids Loading ◽  
High Solids Loading ◽  
Hydrolysis Of

Enzymatic hydrolysis of cactus pear varieties with high solids loading for bioethanol production

2018 ◽  
Vol 250 ◽  
pp. 273-280 ◽  
Author(s):  
Bárbara Ribeiro Alves Alencar ◽  
Emmanuel Damilano Dutra ◽  
Everardo Valadares de Sá Barretto Sampaio ◽  
Rômulo Simões Cezar Menezes ◽  
Marcos Antônio Morais
Keyword(s):  
Enzymatic Hydrolysis ◽  
Bioethanol Production ◽  
High Solids ◽  
Cactus Pear ◽  
Solids Loading ◽  
High Solids Loading ◽  
Hydrolysis Of

scholarly journals Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: a critical review

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Ayla Sant’Ana da Silva ◽  
Roberta Pereira Espinheira ◽  
Ricardo Sposina Sobral Teixeira ◽  
Marcella Fernandes de Souza ◽  
Viridiana Ferreira-Leitão ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Critical Review ◽  
High Solids ◽  
Hydrolysis Of

scholarly journals A high solids field-to-fuel research pipeline to identify interactions between feedstocks and biofuel production

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Meenaa Chandrasekar ◽  
Leela Joshi ◽  
Karleigh Krieg ◽  
Sarvada Chipkar ◽  
Emily Burke ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Environmental Factors ◽  
Shake Flask ◽  
Biofuel Production ◽  
High Solids ◽  
Roller Bottle ◽  
Solids Loading ◽  
High Solids Loading ◽  
Afex Pretreatment ◽  
Roller Bottles

Abstract Background Environmental factors, such as weather extremes, have the potential to cause adverse effects on plant biomass quality and quantity. Beyond adversely affecting feedstock yield and composition, which have been extensively studied, environmental factors can have detrimental effects on saccharification and fermentation processes in biofuel production. Only a few studies have evaluated the effect of these factors on biomass deconstruction into biofuel and resulting fuel yields. This field-to-fuel evaluation of various feedstocks requires rigorous coordination of pretreatment, enzymatic hydrolysis, and fermentation experiments. A large number of biomass samples, often in limited quantity, are needed to thoroughly understand the effect of environmental conditions on biofuel production. This requires greater processing and analytical throughput of industrially relevant, high solids loading hydrolysates for fermentation, and led to the need for a laboratory-scale high solids experimentation platform. Results A field-to-fuel platform was developed to provide sufficient volumes of high solids loading enzymatic hydrolysate for fermentation. AFEX pretreatment was conducted in custom pretreatment reactors, followed by high solids enzymatic hydrolysis. To accommodate enzymatic hydrolysis of multiple samples, roller bottles were used to overcome the bottlenecks of mixing and reduced sugar yields at high solids loading, while allowing greater sample throughput than possible in bioreactors. The roller bottle method provided 42–47% greater liquefaction compared to the batch shake flask method for the same solids loading. In fermentation experiments, hydrolysates from roller bottles were fermented more rapidly, with greater xylose consumption, but lower final ethanol yields and CO2 production than hydrolysates generated with shake flasks. The entire platform was tested and was able to replicate patterns of fermentation inhibition previously observed for experiments conducted in larger-scale reactors and bioreactors, showing divergent fermentation patterns for drought and normal year switchgrass hydrolysates. Conclusion A pipeline of small-scale AFEX pretreatment and roller bottle enzymatic hydrolysis was able to provide adequate quantities of hydrolysate for respirometer fermentation experiments and was able to overcome hydrolysis bottlenecks at high solids loading by obtaining greater liquefaction compared to batch shake flask hydrolysis. Thus, the roller bottle method can be effectively utilized to compare divergent feedstocks and diverse process conditions.

scholarly journals Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Saisi Xue ◽  
Nirmal Uppugundla ◽  
Michael J. Bowman ◽  
David Cavalier ◽  
Leonardo Da Costa Sousa ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzyme Inhibition ◽  
High Solids ◽  
Solids Loading ◽  
High Solids Loading ◽  
Sugar Loss

scholarly journals A clean and effective potassium hydroxide pretreatment of corncob residue for the enhancement of enzymatic hydrolysis at high solids loading

RSC Advances ◽  
2019 ◽  
Vol 9 (20) ◽  
pp. 11558-11566 ◽  
Author(s):  
Xuewen Chi ◽  
Chao Liu ◽  
Yan-Hong Bi ◽  
Guang Yu ◽  
Yuedong Zhang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Potassium Hydroxide ◽  
Solid Loading ◽  
High Solids ◽  
High Solid ◽  
High Solid Loading ◽  
Solids Loading ◽  
High Solids Loading

The effective KOH pretreatment with the production of lignin-based fertilizer could well match the enzymatic hydrolysis at high solid loading.

Recycling cellulases by pH-triggered adsorption-desorption during the enzymatic hydrolysis of lignocellulosic biomass

2014 ◽  
Vol 98 (12) ◽  
pp. 5765-5774 ◽  
Author(s):  
Yaping Shang ◽  
Rongxin Su ◽  
Renliang Huang ◽  
Yang Yang ◽  
Wei Qi ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Adsorption Desorption ◽  
Hydrolysis Of

Large-scale, high-solids enzymatic hydrolysis of steam-exploded poplar

2011 ◽  
Vol 5 (6) ◽  
pp. 609-620 ◽  
Author(s):  
S. Di Risio ◽  
C. S. Hu ◽  
B. A. Saville ◽  
D. Liao ◽  
J. Lortie
Keyword(s):  
Enzymatic Hydrolysis ◽  
Large Scale ◽  
High Solids ◽  
Hydrolysis Of
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