Mass spectrometry hyphenated techniques for the analysis of volatiles and peptides in soft cheese: Useful tools for the shelf life optimization

2016 ◽  
Vol 37 (13) ◽  
pp. 1861-1872 ◽  
Author(s):  
Annalisa Mentana ◽  
Anna Natale ◽  
Carmen Palermo ◽  
Donatella Nardiello ◽  
Amalia Conte ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Shelf Life ◽  
Hyphenated Techniques ◽  
Soft Cheese

Species-selective Analysis for Metal - Biomacromolecular Complexes using Hyphenated Techniques

1999 ◽  
Vol 71 (5) ◽  
pp. 899-918 ◽  
Author(s):  
J. Szpunar ◽  
R. Lobinski
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Absorption Spectrometry ◽  
Reversed Phase ◽  
Size Exclusion ◽  
Emission Spectrometry ◽  
Hyphenated Techniques ◽  
Plasma Atomic Emission Spectrometry ◽  
Icp Ms ◽  
On Line

Analytical chemistry of metal complexes with biomacromolecules based on the coupling of a high resolution separation technique with an element or species selective detection technique is critically discussed. The role of size-exclusion chromatography (SEC) with on-line atomic spectrometric detection is evaluated for the characterization of the metal distribution among the fractions of different molecular weight. Attention is given to the conditions for the separation of metallated biomacromolecular isoforms and sub-isoforms by anion-exchange and reversed-phase HPLC. Techniques for interfacing chromatography with atomic absorption spectrometry (AAS), inductively coupled plasma atomic emission spectrometry (ICP AES) and ICP mass spectrometry (ICP MS) are assessed. The potential of electrospray (tandem) mass spectrometry for the on-line determination of the molecular mass of the eluting protein is highlighted. Perspectives for capillary zone electrophoresis (CZE), microbore and capillary HPLC with ICP MS and electrospray MS detection for probing metalloproteins are discussed. Applications of hyphenated techniques to the analysis of real-world samples are reviewed.

scholarly journals APPLICATION OF LIQUID CHROMATOGRAPHY COUPLED WITH MASS SPECTROMETRY IN THE IMPURITY PROFILING OF DRUG SUBSTANCES AND PRODUCTS

2018 ◽  
Vol 11 (5) ◽  
pp. 30
Author(s):  
Akshatha H S ◽  
Gurupadayya Bm
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Sensitivity ◽  
Pharmaceutical Formulations ◽  
Active Pharmaceutical Ingredient ◽  
Hyphenated Techniques ◽  
Drug Substances ◽  
Impurity Profiling ◽  
Short Period

As the drug safety and efficacy is hampered in the presence of an impurity, the international regulatory agencies laid down stringent limits for the control of impurities in the active pharmaceutical ingredient and pharmaceutical formulations. The conventional approaches lack the characterization of impurities in trace levels, due to sensitivity issues, hyphenated techniques are preferred. Among the modern hyphenated techniques, liquid chromatography-mass spectrometry (LC-MS) has high sensitivity and can analyze large number of organic compounds in a short period of time. In the present study, the impurity profiling of various drug substances and products using LC-MS about past 6 years were retrospect for its importance, instrumentations, and applications.

Recent development in mass spectrometry and its hyphenated techniques for the analysis of medicinal plants

2018 ◽  
Vol 29 (4) ◽  
pp. 365-374 ◽  
Author(s):  
Ming-Zhi Zhu ◽  
Gui-Lin Chen ◽  
Jian-Lin Wu ◽  
Na Li ◽  
Zhong-Hua Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Medicinal Plants ◽  
Hyphenated Techniques

scholarly journals How Coffee Capsules Affect the Volatilome in Espresso Coffee

Separations ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 248
Author(s):  
Giuseppe Greco ◽  
Estefanía Núñez-Carmona ◽  
Marco Abbatangelo ◽  
Patrizia Fava ◽  
Veronica Sberveglieri
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Shelf Life ◽  
Volatile Compounds ◽  
Espresso Coffee ◽  
Coffee Machine ◽  
Related Sample

Coffee capsules have become one of the most used methods to have a coffee in the last few years. In this work, coffee was prepared using a professional espresso coffee machine. We investigated the volatilome of four different polypropylene coffee capsule typologies (Biologico, Dolce, Deciso, Guatemala) with and without capsules in order to reveal the possible differences in the VOCs spectra. The volatilome of each one was singularly studied through an analysis by gas chromatography and mass spectrometry (GC–MS), checking the abundance of different VOCs in coffee extracted with and without a capsule protection and compared to its related sample. Furthermore, ANOVA and Tukey tests were applied to statistically identify and individuate the possible differences. As a result, it was found that coffee capsules, offer advantages of protecting coffee from oxidation or rancidity and, consequently extended shelf life as well as did not cause a reduction of volatile compounds intensity. Therefore, it is possible to conclude that the aroma of polypropylene coffee capsule extraction is not damaged compared to a traditional espresso.

scholarly journals Vitamin B12 determination in milk, whey and different by-products of ricotta cheese production by ultra performance liquid chromatography coupled with tandem mass spectrometry

2017 ◽  
Vol 6 (4) ◽  
Author(s):  
Adele Repossi ◽  
Elisa Zironi ◽  
Teresa Gazzotti ◽  
Andrea Serraino ◽  
Giampiero Pagliuca
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Vitamin B12 ◽  
Shelf Life ◽  
Light Exposure ◽  
Early Stage ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Cheese Making

Vitamin B12 (cobalamin) is a metal complex composed of a central cobalt ion bonded to six ligands. It is essential for major biological functions such as protein, fat and carbohydrate metabolism, the maintenance of the central nervous system, and the formation of red blood cells. Since mammals cannot synthesize cobalamin, dietary intake represents the only natural source for humans. Dairy products can provide significant levels of cobalamin; moreover, the European Food Safety Authority (EFSA) panel has set the recommended intake at 4 μg/day for adults. Vitamin B12 content was determined in milk and several matrices related to the process of transformation of the residual whey from Parmigiano Reggiano cheese-making to obtain ricotta cheese. In addition, vitamin B12 degradation during ricotta cheese shelf-life was studied. The analyses were performed using an ultra performance liquid chromatography-tandem mass spectrometry method. Results show that vitamin B12 amount in ricotta from dairy and experimental cheese-making brings respectively 1/8 to 1/4 of the adequate intake in adults established by EFSA. In addition, shelf-life experiment shows that cobalamine is fairly rapidly degraded in ricotta: light effect seems to be significant, even if the light exposure is short. The use of photoprotective packaging material increases B12 shelf-life in the early stage of storage.

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