Enhancement of enantioselectivity in chiral capillary electrophoresis using hydroxypropyl-beta-cyclodextrin as chiral selector under molecular crowding conditions induced by dextran or dextrin

2014 ◽  
Vol 35 (20) ◽  
pp. 2938-2945 ◽  
Author(s):  
Qifeng Fu ◽  
Fengqing Yang ◽  
Hua Chen ◽  
Zhining Xia
Keyword(s):  
Capillary Electrophoresis ◽  
Chiral Selector ◽  
Molecular Crowding ◽  
Beta Cyclodextrin ◽  
Chiral Capillary Electrophoresis ◽  
Hydroxypropyl Beta Cyclodextrin

scholarly journals Study of Cyclodextrin/Fluoroquinolone Inclusion Complexes by Capillary Electrophoresis

2013 ◽  
Vol 59 (2) ◽  
pp. 107-110 ◽  
Author(s):  
Blanka Székely-Szentmiklósi ◽  
B Tőkés
Keyword(s):  
Capillary Electrophoresis ◽  
Inclusion Complexes ◽  
Host Interactions ◽  
Beta Cyclodextrin ◽  
Experimental Parameters ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
New Formulation ◽  
Hydroxypropyl Beta Cyclodextrin

AbstractIntroduction: In the present work we evaluated the complexation role of cyclodextrins toward fluoroquinolones in an attempt to assess their potential as new formulation additives for more efficient fluoroquinolone delivery and as selectors in capillary electrophoresis.Material and method: Guest-host interactions of two second generation quinolones, ciprofloxacin and norfloxacin with four cyclodextrins, beta-cyclodextrin (β-CD), gamma-cyclodextrin (γ-CD) and two beta-cyclodextrin derivatives, 2-hydroxypropyl beta-cyclodextrin (HP-β-CD) and randomly methylated beta-cyclodextrin (RAMEB), were tested by capillary electrophoresis in borate running buffer. Experimental parameters like buffer concentration, pH, organic modifier, voltage and cyclodextrin concentration have been varied for a better resolution.Results: In capillary zone electrophoresis ciprofloxacin and norfloxacin are migrating together, a difference in their migration times and thus separation occured by the addition of cyclodextrins.Conclusion: Our results suggest formation of inclusion complexes between fluoroquinolones and cyclodextrins. Differences in their affinity to host molecules resulted in separation of the two fluoroquinolones

Chiral capillary electrophoresis–electrospray mass spectrometry coupling using vancomycin as chiral selector

1998 ◽  
Vol 800 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Salvatore Fanali ◽  
Claudia Desiderio ◽  
Georg Schulte ◽  
Stefan Heitmeier ◽  
Dirk Strickmann ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Electrospray Mass Spectrometry ◽  
Chiral Selector ◽  
Chiral Capillary Electrophoresis

scholarly journals Enantioselective Study on the Biodegradation of Verapamil and Cytalopram by Chiral Capillary Electrophoresis

Separations ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 29
Author(s):  
Yolanda Martín-Biosca ◽  
Laura Escuder-Gilabert ◽  
Salvador Sagrado ◽  
María José Medina-Hernández
Keyword(s):  
Capillary Electrophoresis ◽  
Chiral Selector ◽  
Minimal Salt Medium ◽  
Salt Medium ◽  
Chiral Drugs ◽  
Batch Mode ◽  
Chiral Compounds ◽  
Complete Filling ◽  
Abiotic Degradation ◽  
Chiral Capillary Electrophoresis

Many of the currently available drugs are chiral compounds that are marketed as racemates or, to a lesser extent, in the form of one of the enantiomers since a pair of enantiomers may have different toxicological and ecotoxicological properties compared to each other. The evaluation of enantioselectivity in biodegradation processes is essential for environmental risk assessment. The objective of this research is to study the enantioselectivity in the biodegradation of two common chiral drugs, citalopram and verapamil, using highly sulphated-γ-cyclodextrin (HS-γ-CD) as chiral selector in Capillary Electrophoresis. Biodegradation experiments were performed in batch mode using a minimal salt medium inoculated with an activated sludge and supplemented with the corresponding enantiomeric mixture. The cultures were incubated at 20 °C for 28 days. Abiotic degradation of verapamil and citalopram enantiomers was also assessed. The concentration of the enantiomers of verapamil and citalopram were monitored using 0.7% and 0.1% m/v HS-γ-CD solutions as chiral selector, respectively. Separations were carried out using the complete filling technique. The results of biodegradability tests indicate that citalopram could be considered potentially persistent while verapamil is presumed to be a non-persistent compound. No evidence of enantioselectivity was observed in any of the biodegradation processes.

Characterization of a single-isomer carboxymethyl-beta-cyclodextrin in chiral capillary electrophoresis

2017 ◽  
Vol 38 (15) ◽  
pp. 1869-1877 ◽  
Author(s):  
Ida Fejős ◽  
Erzsébet Varga ◽  
Gábor Benkovics ◽  
Milo Malanga ◽  
Tamás Sohajda ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Single Isomer ◽  
Beta Cyclodextrin ◽  
Chiral Capillary Electrophoresis

Determination of enantiomeric excess for 2,3-dihydroxy-3-phenylpropionate compounds by capillary electrophoresis using hydroxypropyl-β-cyclodextrin as chiral selector

Chirality ◽  
2007 ◽  
Vol 19 (5) ◽  
pp. 380-385 ◽  
Author(s):  
Yan Zhao ◽  
Xing-Bin Yang ◽  
Qiao-Feng Wang ◽  
Peng-Juan Nan ◽  
Ying Jin ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Enantiomeric Excess ◽  
Chiral Selector
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