scholarly journals A real-time impedance-sensing chip for the detection of emulsion phase separation

2013 ◽  
Vol 34 (12) ◽  
pp. NA-NA ◽  
Author(s):  
Yung-Sheng Lin ◽  
Wei-Lung Chou ◽  
Chih-Hui Yang ◽  
Keng-Shiang Huang ◽  
Eng-Chi Wang ◽  
...  
Keyword(s):  
Phase Separation ◽  
Real Time ◽  
Impedance Sensing ◽  
Emulsion Phase

A real-time impedance-sensing chip for the detection of emulsion phase separation

2013 ◽  
Vol 34 (12) ◽  
pp. 1743-1748 ◽  
Author(s):  
Yung-Sheng Lin ◽  
Wei-Lung Chou ◽  
Chih-Hui Yang ◽  
Keng-Shiang Huang ◽  
Eng-Chi Wang ◽  
...  
Keyword(s):  
Phase Separation ◽  
Real Time ◽  
Impedance Sensing ◽  
Emulsion Phase

scholarly journals Electric Cell-Substrate Impedance Sensing To Monitor Viral Growth and Study Cellular Responses to Infection with Alphaherpesviruses in Real Time

mSphere ◽  
2017 ◽  
Vol 2 (2) ◽  
Author(s):  
Matthew R. Pennington ◽  
Gerlinde R. Van de Walle
Keyword(s):  
Real Time ◽  
Cellular Response ◽  
Cellular Responses ◽  
Cellular Damage ◽  
Impedance Sensing ◽  
Novel Technologies ◽  
Antiviral Therapies ◽  
Mucosal Surfaces ◽  
Cell Substrate ◽  
Hsv 1

ABSTRACT Alphaherpesviruses, including those that commonly infect humans, such as HSV-1 and HSV-2, typically infect and cause cellular damage to epithelial cells at mucosal surfaces, leading to disease. The development of novel technologies to study the cellular responses to infection may allow a more complete understanding of virus replication and the creation of novel antiviral therapies. This study demonstrates the use of ECIS to study various aspects of herpesvirus biology, with a specific focus on changes in cellular morphology as a result of infection. We conclude that ECIS represents a valuable new tool with which to study alphaherpesvirus infections in real time and in an objective and reproducible manner. Electric cell-substrate impedance sensing (ECIS) measures changes in an electrical circuit formed in a culture dish. As cells grow over a gold electrode, they block the flow of electricity and this is read as an increase in electrical impedance in the circuit. ECIS has previously been used in a variety of applications to study cell growth, migration, and behavior in response to stimuli in real time and without the need for cellular labels. Here, we demonstrate that ECIS is also a valuable tool with which to study infection by alphaherpesviruses. To this end, we used ECIS to study the kinetics of cells infected with felid herpesvirus type 1 (FHV-1), a close relative of the human alphaherpesviruses herpes simplex virus 1 (HSV-1) and HSV-2, and compared the results to those obtained with conventional infectivity assays. First, we demonstrated that ECIS can easily distinguish between wells of cells infected with different amounts of FHV-1 and provides information about the cellular response to infection. Second, we found ECIS useful in identifying differences between the replication kinetics of recombinant DsRed Express2-labeled FHV-1, created via CRISPR/Cas9 genome engineering, and wild-type FHV-1. Finally, we demonstrated that ECIS can accurately determine the half-maximal effective concentration of antivirals. Collectively, our data show that ECIS, in conjunction with current methodologies, is a powerful tool that can be used to monitor viral growth and study the cellular response to alphaherpesvirus infection. IMPORTANCE Alphaherpesviruses, including those that commonly infect humans, such as HSV-1 and HSV-2, typically infect and cause cellular damage to epithelial cells at mucosal surfaces, leading to disease. The development of novel technologies to study the cellular responses to infection may allow a more complete understanding of virus replication and the creation of novel antiviral therapies. This study demonstrates the use of ECIS to study various aspects of herpesvirus biology, with a specific focus on changes in cellular morphology as a result of infection. We conclude that ECIS represents a valuable new tool with which to study alphaherpesvirus infections in real time and in an objective and reproducible manner.

scholarly journals Comparison of Leading Biosensor Technologies to Detect Changes in Human Endothelial Barrier Properties in Response to Pro-Inflammatory TNFα and IL1β in Real-Time

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 159
Author(s):  
James J. W. Hucklesby ◽  
Akshata Anchan ◽  
Simon J. O'Carroll ◽  
Charles P. Unsworth ◽  
E. Scott Graham ◽  
...  
Keyword(s):  
Human Brain ◽  
Real Time ◽  
Barrier Properties ◽  
Endothelial Barrier ◽  
Endothelial Monolayer ◽  
Impedance Sensing ◽  
Endothelial Monolayers ◽  
Impedance Data ◽  
Cell Substrate ◽  
Limited Frequency

Electric Cell-Substrate Impedance Sensing (ECIS), xCELLigence and cellZscope are commercially available instruments that measure the impedance of cellular monolayers. Despite widespread use of these systems individually, direct comparisons between these platforms have not been published. To compare these instruments, the responses of human brain endothelial monolayers to TNFα and IL1β were measured on all three platforms simultaneously. All instruments detected transient changes in impedance in response to the cytokines, although the response magnitude varied, with ECIS being the most sensitive. ECIS and cellZscope were also able to attribute responses to particular endothelial barrier components by modelling the multifrequency impedance data acquired by these instruments; in contrast the limited frequency xCELLigence data cannot be modelled. Consistent with its superior impedance sensing, ECIS exhibited a greater capacity than cellZscope to distinguish between subtle changes in modelled endothelial monolayer properties. The reduced resolving ability of the cellZscope platform may be due to its electrode configuration, which is necessary to allow access to the basolateral compartment, an important advantage of this instrument. Collectively, this work demonstrates that instruments must be carefully selected to ensure they are appropriate for the experimental questions being asked when assessing endothelial barrier properties.

CO2 switchable deep eutectic solvents for reversible emulsion phase separation

2021 ◽  
Author(s):  
Feijie Liu ◽  
Zhimin Xue ◽  
Xue Lan ◽  
Zhenghui Liu ◽  
Tiancheng Mu
Keyword(s):  
Phase Separation ◽  
Deep Eutectic Solvents ◽  
Emulsion Phase ◽  
Reversible Phase

CO2 switchable imidazole-based deep eutectic solvents (DESs) were formed and used for reversible phase separation of emulsions generated between DESs and oil.

In situ photon transmission technique for monitoring phase separation in real time during gelation

2000 ◽  
Vol 45 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Selim Kara ◽  
Oguz Okay ◽  
Önder Pekcan
Keyword(s):  
Phase Separation ◽  
Real Time ◽  
Transmission Technique ◽  
Monitoring Phase

Real-time cell-impedance sensing assay as an alternative to clonogenic assay in evaluating cancer radiotherapy

2011 ◽  
Vol 400 (7) ◽  
pp. 2003-2011 ◽  
Author(s):  
Wilson Roa ◽  
Xiaoyan Yang ◽  
Linghong Guo ◽  
Biao Huang ◽  
Shima Khatibisepehr ◽  
...  
Keyword(s):  
Real Time ◽  
Clonogenic Assay ◽  
Cancer Radiotherapy ◽  
Impedance Sensing ◽  
Cell Impedance
Sign in / Sign up

Export Citation Format

Share Document