A novel capillary electrophoretic method for determining aliphatic aldehydes in food samples using 2-thiobarbituric acid derivatization

2011 ◽  
Vol 32 (6-7) ◽  
pp. 705-711 ◽  
Author(s):  
Jun-Bo Zhang ◽  
Meng-Jie Li ◽  
Wen-Li Li ◽  
Qing-Cui Chu ◽  
Jian-Nong Ye
Keyword(s):  
Thiobarbituric Acid ◽  
Food Samples ◽  
Aliphatic Aldehydes ◽  
Electrophoretic Method ◽  
Capillary Electrophoretic

scholarly journals Capillary Electrophoretic-Ultraviolet Method for the Separation and Estimation of Zineb, Maneb, and Ferbam in Food Samples

2007 ◽  
Vol 90 (3) ◽  
pp. 834-837 ◽  
Author(s):  
Jatinder Singh Aulakh ◽  
Agnes Fekete ◽  
Ashok Kumar Malik ◽  
Rakesh Kumar Mahajan ◽  
Philippe Schmitt-Kopplin
Keyword(s):  
Signal To Noise Ratio ◽  
Food Samples ◽  
Tetraacetic Acid ◽  
Signal To Noise ◽  
Electrophoretic Method ◽  
Ultraviolet Detection ◽  
Buffer Solutions ◽  
Relative Standard ◽  
Capillary Electrophoretic

Abstract A simple and sensitive capillary electrophoretic method with ultraviolet detection has been developed for the separation and determination of ferbam [iron(III)-dimethyldithiocarbamate], maneb [manganese(II)-ethylenebisdithiocarbamate] and zineb [zinc(II)-ethylenebisdithiocarbamate], in borate buffer, after their acidic decomposition and complexation with CDTA (trans-1,2-diaminocyclohexane-N,N,N,N-tetraacetic acid monohydrate), as CDTA-metal complexes of Fe+3, Mn+2, and Zn+2. The determination is dependent on the pH and the nature of the buffer solutions. In this method, the detection limit (signal-to-noise ratio = 3) is 0.0013, 0.0022, and 0.0023 mM for ferbam, maneb, and zineb, respectively. The relative standard deviation for the analysis of 1 mM of each was found to be 1.5 0.2%. The method was successfully applied for the analysis of red beans and grain samples spiked with ferbam, maneb, and zineb. The applicability of capillary electrophoresis as a useful tool for the simultaneous determination and analysis of ferbam, maneb, and zineb is demonstrated.

Separation of Sucralose in Food Samples using Amines as Background Electrolyte Supported with DFT Calculations

2020 ◽  
Vol 16 ◽  
Author(s):  
Bathinapatla Ayyappa ◽  
Suvardhan Kanchi ◽  
Myalowenkosi I. Sabela ◽  
Krishna Bisetty
Keyword(s):  
Background Electrolyte ◽  
Food Additives ◽  
Daily Intake ◽  
Food Samples ◽  
Uv Detection ◽  
Migration Time ◽  
Artificial Sweetener ◽  
Buffer Concentration ◽  
Electrophoretic Method ◽  
Amine Buffers

BACKGROUND: Sucralose is a high intensity artificial sweetener sucralose and chemically known as 1,6-dichloro-1,6-dideoxy-β-D-fructofuranosyl-4-chloro-4-deoxy-α-D-galactopyranoside. It is used as a sweetener and flavour enhancer in foods and beverages. Due to its high stability at wider temperatures and pH, made its applicability in various food products throughout the world. As per Joint FAO/WHO Expert Group on Food Additives (JECFA) in 1990, the daily intake of sucralose is 0-15 mg/kg body weight. The literature reports suggest that sucralose has a possible health threat due to the presence of chlorine groups, thereby leading to the several illnesses. The growing interest on the use of SUC in the foods, makes it necessary in developing a fast, reliable, cost effective and reproducible analytical method to determine SUC in food samples. The detection of sucralose and other carbohydrates like fructose, glucose and sucrose is a challenging task owing to its: (i) unavailability of the charged functions and (ii) lack of absorption of strong chromophoric nature in the UV region. Therefore, separation of non-absorbing neutral molecules needs a careful procedure with the suitable electrolyte systems. METHODOLOGY: An indirect UV detection capillary electrophoretic method is described for the separation of sucralose in different food samples. It was achieved by nucleophile substitution (SN2) in the presence of amine as background electrolytes. The morpholine buffer showed good buffering capacity in terms of migration time (< 8.0 min) and baseline stability when compared to other amine buffers (ethylamine, piperidine, triethylamine). The analytical applications of proposed method showed by recovery percentages of sucralose in real and spiked samples on intra and inter-day basis at optimum experimental conditions of 0.2 M buffer concentration and pH 12.0 at 230 nm UV detection. RESULTS: The selection of BGE, UV detection wavelength, buffer concentration, buffer pH, cassette temperature and applied voltage were optimized to enhance the sensitivity and selectivity of the separation method. Recoveries obtained were ranging from 96.87 to 98.82 % for real samples and 94.45 to 98.06 % for spiked samples respectively. Linearity was studied in the range of 2-10 mM, and showed a correlation coefficients of 0.9942 with LOD and LOQ found to be 0.3804 mg L-1 and 1.5215 mg L-1 with % RSD (n = 5) ± 1.27 and 1.19 % with respect to migration time and peak area. Furthermore, to better understand the separation of sucralose with amine buffers were investigated computationally using HOMO-LUMO calculations. The obtained results showed that the band gap decreases in the presence of amine moiety irrespective of its nature. CONCLUSION: In the study, novel background electrolytic system was successfully applied to separate sucralose using indirect UV detector with capillary electrophoresis. The FT-IR results confirmed that the interaction of sucralose with different amine buffers to better understand the separation chemistry behind sucralose and amine complexes. Moreover, computational results indicate that the direction of charge transfer from the amine functionality to the glucofuranosyl ring in each amine derivative of sucralose confirms the strong interaction between sucralose and amines, which led in the baseline separation of sucralose in different food samples.

Semiautomatic Determination of Furanic Aldehydes in Food and Pharmaceutical Samples by a Stopped-Flow Injection Analysis Method

1993 ◽  
Vol 76 (6) ◽  
pp. 1255-1261 ◽  
Author(s):  
A Espinosa-Mansilla ◽  
A Muñoz Dela Peña ◽  
F Salinas
Keyword(s):  
Flow Injection ◽  
Flow Injection Analysis ◽  
Pharmaceutical Preparations ◽  
Thiobarbituric Acid ◽  
Food Samples ◽  
Analytical Determination ◽  
Stopped Flow ◽  
Analysis Method ◽  
Analysis Technique

Abstract A kinetic study of the reactions of 5-hydroxymethyl-2-furfuraldehyde and furfural with 2-thiobarbituric acid (TBA) by a stopped-flow flow injection analysis technique has been undertaken. A semiautomatic method for the analytical determination of these furanic aldehydes is proposed on the basis of reaction with TBA. The proposed stopped-flow method was successfully applied to several commercial pharmaceutical preparations and food samples. The procedure is faster than the earlier procedure for determination of these compounds in foods and pharmaceuticals.

Capillary electrophoretic method for the determination of diterpenoid isomers in Acanthopanax species

2006 ◽  
Vol 40 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Nguyen Thi Phuong ◽  
Kyung Ah Lee ◽  
Su Jin Jeong ◽  
Cai Xing Fu ◽  
Jung Kap Choi ◽  
...  
Keyword(s):  
Electrophoretic Method ◽  
Capillary Electrophoretic ◽  
Acanthopanax Species
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