An expanding negative detection method for the visualization of DNA in polyacrylamide gels by eosin Y

2010 ◽  
Vol 31 (20) ◽  
pp. 3450-3456 ◽  
Author(s):  
Zhong-Xin Zhu ◽  
Wei-Tao Cong ◽  
Peng-Hui Zhang ◽  
Wei-De Ma ◽  
Liu Min ◽  
...  
Keyword(s):  
Detection Method ◽  
Eosin Y ◽  
Polyacrylamide Gels ◽  
Negative Detection

Advanced negative detection method comparable to silver stain for SDS-PAGE separated proteins detection

2016 ◽  
Vol 510 ◽  
pp. 21-25 ◽  
Author(s):  
Xu Wang ◽  
Sun-Young Hwang ◽  
Wei-Tao Cong ◽  
Li-Tai Jin ◽  
Jung-Kap Choi
Keyword(s):  
Detection Method ◽  
Silver Stain ◽  
Sds Page ◽  
Negative Detection

Eosin Y staining of proteins in polyacrylamide gels

1991 ◽  
Vol 196 (2) ◽  
pp. 279-283 ◽  
Author(s):  
Fan Lin ◽  
Wei Fan ◽  
Gary E. Wise
Keyword(s):  
Eosin Y ◽  
Polyacrylamide Gels

scholarly journals In Situ Detection Method for Glutaminyl Cyclase Activity in Polyacrylamide Gels

BioTechniques ◽  
1998 ◽  
Vol 24 (3) ◽  
pp. 346-348
Author(s):  
Justin B. Houseknecht ◽  
Jeffrey S. Temple ◽  
Robert C. Bateman
Keyword(s):  
Detection Method ◽  
Cyclase Activity ◽  
Polyacrylamide Gels ◽  
Glutaminyl Cyclase ◽  
In Situ Detection

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

Sign in / Sign up

Export Citation Format

Share Document