scholarly journals In Situ Detection Method for Glutaminyl Cyclase Activity in Polyacrylamide Gels

BioTechniques ◽  
1998 ◽  
Vol 24 (3) ◽  
pp. 346-348
Author(s):  
Justin B. Houseknecht ◽  
Jeffrey S. Temple ◽  
Robert C. Bateman
Keyword(s):  
Detection Method ◽  
Cyclase Activity ◽  
Polyacrylamide Gels ◽  
Glutaminyl Cyclase ◽  
In Situ Detection

scholarly journals Vacuum Leak Detection Method Using Index Regression and Correction for Semiconductor Equipment in a Vacuum Chamber

2021 ◽  
Vol 11 (24) ◽  
pp. 11762
Author(s):  
Taekyung Ha ◽  
Hyunjung Shin
Keyword(s):  
Vacuum Chamber ◽  
Detection Method ◽  
Pressure Control ◽  
Leak Detection ◽  
Process Yield ◽  
Mass Spectrometers ◽  
Detection Systems ◽  
Isolation Condition ◽  
In Situ Detection

In semiconductor manufacturing, fault detection is an important method for monitoring equipment condition and examining the potential causes of a fault. Vacuum leakage is considered one of the major faults that can occur in semiconductor processing. An unnecessary O2 and N2 mixture, a major component of the atmosphere, creates unexpected process results and hence drops in yield. Vacuum leak detection systems that are currently available in the vacuum industry are based on helium mass spectrometers. They are used for detecting the vacuum leakage at the sole isolation condition where the chamber is fully pumped but cannot be used for in situ detection while the process is ongoing in the chamber. In this article, a chamber vacuum leak detection method named Index Regression and Correction (IRC) is presented, utilizing common data which were gathered during normal chamber operation. This method was developed by analyzing a simple list of data, such as pressure, the temperature of the chamber body, and the position of the auto pressure control (APC), to detect any leakages in the vacuum chamber. The proposed method was experimentally verified and the results showed a high accuracy of up to 97% when a vacuum leak was initiated in the chamber. The proposed method is expected to improve the process yield of the chamber by detecting even small vacuum leakages at very early stages of the process.

High-accuracy in-situ detection method of monochromator angular vibration

2020 ◽  
Vol 13 (1) ◽  
pp. 156-164 ◽  
Author(s):  
樊奕辰 FAN Yi-chen ◽  
李中亮 LI Zhong-liang ◽  
徐中民 XU Zhong-min ◽  
张 琦 ZHANG Qi ◽  
刘 运 LIU Yun ◽  
...  
Keyword(s):  
Detection Method ◽  
High Accuracy ◽  
Angular Vibration ◽  
In Situ Detection

In situ detection method for obtaining permeability of Fe-based amorphous alloys: ac resistance measurement for Fe84Nb7B9

2005 ◽  
Vol 86 (3) ◽  
pp. 032503 ◽  
Author(s):  
Tetsu Ichitsubo ◽  
Eiichiro Matsubara ◽  
Satoshi Tanaka ◽  
Nobuyuki Nishiyama ◽  
Kenji Amiya
Keyword(s):  
Amorphous Alloys ◽  
Detection Method ◽  
Resistance Measurement ◽  
In Situ Detection

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

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