scholarly journals A multichannel gel electrophoresis and continuous fraction collection apparatus for high-throughput protein separation and characterization

2010 ◽  
Vol 31 (3) ◽  
pp. 440-447 ◽  
Author(s):  
Megan Choi ◽  
Robert A. Nordmeyer ◽  
Earl Cornell ◽  
Ming Dong ◽  
Mark D. Biggin ◽  
...  
Keyword(s):  
High Throughput ◽  
Gel Electrophoresis ◽  
Protein Separation ◽  
Continuous Fraction ◽  
Fraction Collection

High-throughput method for determination of apolipoprotein E genotypes with use of restriction digestion analysis by microplate array diagonal gel electrophoresis

1995 ◽  
Vol 41 (11) ◽  
pp. 1599-1604 ◽  
Author(s):  
M K Bolla ◽  
L Haddad ◽  
S E Humphries ◽  
A F Winder ◽  
I N Day
Keyword(s):  
Apolipoprotein E ◽  
High Throughput ◽  
Gel Electrophoresis ◽  
Detection Sensitivity ◽  
High Yield ◽  
Restriction Digestion ◽  
Special Equipment ◽  
Alzheimer Dementia ◽  
Apo E ◽  
Apolipoprotein E Genotypes

Abstract Molecular epidemiological research has identified the association of a common apolipoprotein E (apo E) isoform (E4 as opposed to E3), with risk both of coronary artery disease and of Alzheimer dementia. In addition, the role of apo E genotype (usually E2/E2) in Type III hyperlipidemia is well known. However, both for diagnostic and research purposes, apo E genotyping is cumbersome. The preferred approach is electrophoretic sizing of restriction digestion fragments, enabling simultaneous analysis of the two codons (112 and 158) that represent the six common genotypes (E2/E2; E2/E3; E2/E4; E3/E3; E3/E4; E4/E4). However, the consequent demands of high-yield PCR, high-resolution, high-throughput electrophoresis, and sufficient detection sensitivity have left shortfalls in published protocols. In conjunction with a high-throughput electrophoresis system we described recently, microplate array diagonal gel electrophoresis (MADGE), we have constructed extensively optimized, simplified protocols for DNA isolation from mouthwash samples for PCR setup and high-yield PCR, for restriction digestion, and for subsequent MADGE gel image analysis. The integral system enables one worker to readily undertake apo E genotyping of as many as hundreds of DNA samples per day, without special equipment.

scholarly journals Microplate-array diagonal-gel electrophoresis (MADGE) systems for high-throughput electrophoresis

2000 ◽  
Vol 5 (1) ◽  
pp. 12-18
Author(s):  
Rosalind H. Ganderton ◽  
Sandra D. O'Dell ◽  
Tom R. Gaunt ◽  
Xiao-he Chen ◽  
Lesley J. Hinks ◽  
...  
Keyword(s):  
High Throughput ◽  
Gel Electrophoresis

scholarly journals Combination of His-Tagged T4 Endonuclease VII with Microplate Array Diagonal Gel Electrophoresis for High-Throughput Mutation Scanning

2005 ◽  
Vol 51 (6) ◽  
pp. 1043-1046 ◽  
Author(s):  
Matt J Smith ◽  
Gabriella Pante-de-Sousa ◽  
Khalid K Alharbi ◽  
Xiao-he Chen ◽  
Ian NM Day ◽  
...  
Keyword(s):  
High Throughput ◽  
Gel Electrophoresis ◽  
Mutation Scanning ◽  
Endonuclease Vii

A New Method for Proteome Screening with Two-Dimensional Urea-Sds Polyacrylamide Gel Electrophoresis

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Areeba Ahmad ◽  
Riaz Ahmad
Keyword(s):  
Gel Electrophoresis ◽  
Protein Separation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Cost Effective ◽  
Polyacrylamide Gel ◽  
Present System ◽  
Liver Protein ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

AbstractTwo-dimensional gel electrophoresis (2DE) separating proteins on the basis of their pI and molecular mass remain the best available technique for protein separation and characterization to date. But due to several limitations, including streak formation in IEF gels, partial solubility of proteins, expensive running conditions and relatively longer time taken, a simple urea-SDS-2D polyacrylamide gel electrophoresis (US2DE) is described here. The system is reasonably sensitive, cost effective with good reproducibility. The method described in this paper employs a chaotropic agent, urea, in the first dimension and sodium dodecyl sulphate (SDS), like conventional system, in the second dimension with an addition of polyacrylamide to screen the liver proteome of healthy and chemically induced fibrotic rats. The system separates the protein on the basis of chargeto- mass ratio and clearly demonstrates differential expression in the liver protein repertoire of healthy and fibrotic rats. Moreover, the present system, like other 2D electrophoretic procedures revealed at least 22 novel spots in the investigated tissues. The technique may be utilized for comprehensive proteome screening of any biological sample and would provide an overview to narrow down the candidate proteins or biomarkers.

Sign in / Sign up

Export Citation Format

Share Document