Improved high-throughput DNA fragment analyzer employing horizontal ultrathin gel electrophoresis

1996 ◽  
Author(s):  
Robert L. Brumley, Jr. ◽  
John A. Luckey
Keyword(s):  
High Throughput ◽  
Gel Electrophoresis ◽  
Dna Fragment

scholarly journals Lactobacillus fructivorans Spoilage of Tomato Ketchup

1997 ◽  
Vol 60 (5) ◽  
pp. 505-509 ◽  
Author(s):  
K. JOHANNA BJORKROTH ◽  
HANNU J. KORKEALA
Keyword(s):  
Gel Electrophoresis ◽  
Microbial Growth ◽  
Antimicrobial Agents ◽  
Growth And Survival ◽  
Factors Affecting ◽  
Physiological And Biochemical Characteristics ◽  
Gas Formation ◽  
Tomato Ketchup ◽  
Dna Fragment ◽  
Physiological And Biochemical

Spoilage characterized by bulging as a result of gas formation in bottled ketchup was studied, Samples produced microbial growth on MRS and Rogosa selective Lactobacillus agar. Seventy randomly selected isolates typed by using restriction endonuclease (ClaI, EcoRI, HindIII) analysis were found to have identical DNA fragment patterns in gel electrophoresis. The strain was identified as Lactobacillus fructivorans using morphological, physiological and biochemical characteristics, combined with the information obtained from ribotyping. Factors affecting growth and survival of this L. fructivorans strain in ketchup production were also studied. An L. fructivorans count of 105 CFU/g resulted in spoilage of inoculated ketchup samples. Spoilage occurred only in samples incubated at 15 to 30°C. The L. fructivorans implicated in causing spoilage demonstrated heat resistance with a D value of 1.2 min at 65°C. The strain did not show resistance to alkaline active chloride-containing detergent sanitizer; alkyldimethylbenzylammonium chloride and alkyldimethylethylbenzylammonium chloride-containing sanitizer were also found to be effective antimicrobial agents.

High-throughput method for determination of apolipoprotein E genotypes with use of restriction digestion analysis by microplate array diagonal gel electrophoresis

1995 ◽  
Vol 41 (11) ◽  
pp. 1599-1604 ◽  
Author(s):  
M K Bolla ◽  
L Haddad ◽  
S E Humphries ◽  
A F Winder ◽  
I N Day
Keyword(s):  
Apolipoprotein E ◽  
High Throughput ◽  
Gel Electrophoresis ◽  
Detection Sensitivity ◽  
High Yield ◽  
Restriction Digestion ◽  
Special Equipment ◽  
Alzheimer Dementia ◽  
Apo E ◽  
Apolipoprotein E Genotypes

Abstract Molecular epidemiological research has identified the association of a common apolipoprotein E (apo E) isoform (E4 as opposed to E3), with risk both of coronary artery disease and of Alzheimer dementia. In addition, the role of apo E genotype (usually E2/E2) in Type III hyperlipidemia is well known. However, both for diagnostic and research purposes, apo E genotyping is cumbersome. The preferred approach is electrophoretic sizing of restriction digestion fragments, enabling simultaneous analysis of the two codons (112 and 158) that represent the six common genotypes (E2/E2; E2/E3; E2/E4; E3/E3; E3/E4; E4/E4). However, the consequent demands of high-yield PCR, high-resolution, high-throughput electrophoresis, and sufficient detection sensitivity have left shortfalls in published protocols. In conjunction with a high-throughput electrophoresis system we described recently, microplate array diagonal gel electrophoresis (MADGE), we have constructed extensively optimized, simplified protocols for DNA isolation from mouthwash samples for PCR setup and high-yield PCR, for restriction digestion, and for subsequent MADGE gel image analysis. The integral system enables one worker to readily undertake apo E genotyping of as many as hundreds of DNA samples per day, without special equipment.

scholarly journals Microplate-array diagonal-gel electrophoresis (MADGE) systems for high-throughput electrophoresis

2000 ◽  
Vol 5 (1) ◽  
pp. 12-18
Author(s):  
Rosalind H. Ganderton ◽  
Sandra D. O'Dell ◽  
Tom R. Gaunt ◽  
Xiao-he Chen ◽  
Lesley J. Hinks ◽  
...  
Keyword(s):  
High Throughput ◽  
Gel Electrophoresis

scholarly journals Combination of His-Tagged T4 Endonuclease VII with Microplate Array Diagonal Gel Electrophoresis for High-Throughput Mutation Scanning

2005 ◽  
Vol 51 (6) ◽  
pp. 1043-1046 ◽  
Author(s):  
Matt J Smith ◽  
Gabriella Pante-de-Sousa ◽  
Khalid K Alharbi ◽  
Xiao-he Chen ◽  
Ian NM Day ◽  
...  
Keyword(s):  
High Throughput ◽  
Gel Electrophoresis ◽  
Mutation Scanning ◽  
Endonuclease Vii

scholarly journals High-throughput glycosylation analysis of therapeutic immunoglobulin G by capillary gel electrophoresis using a DNA analyzer

mAbs ◽  
2013 ◽  
Vol 6 (1) ◽  
pp. 185-196 ◽  
Author(s):  
Dietmar Reusch ◽  
Markus Haberger ◽  
Tobias Kailich ◽  
Anna-Katharina Heidenreich ◽  
Michael Kampe ◽  
...  
Keyword(s):  
High Throughput ◽  
Immunoglobulin G ◽  
Gel Electrophoresis ◽  
Capillary Gel Electrophoresis
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