CE-based simultaneous liquid-phase noncompetitive enzyme immunoassay for three tumor markers in human serum using electrochemical detection

2007 ◽  
Vol 28 (23) ◽  
pp. 4427-4434 ◽  
Author(s):  
Shusheng Zhang ◽  
Xuemei Li ◽  
Fan Zhang
Keyword(s):  
Liquid Phase ◽  
Human Serum ◽  
Enzyme Immunoassay ◽  
Tumor Markers ◽  
Electrochemical Detection

A highly sensitive enzyme immunoassay of anti-insulin antibodies in human serum

1987 ◽  
Vol 1 (2) ◽  
pp. 170-174 ◽  
Author(s):  
Takeyuki Kohno ◽  
Eiji Ishikawa ◽  
Satoru Sugiyama ◽  
Syuji Nakamura ◽  
Yoshimasa Kanemaru
Keyword(s):  
Human Serum ◽  
Enzyme Immunoassay ◽  
Insulin Antibodies ◽  
Highly Sensitive

Development of high-performance magnetic chemiluminescence enzyme immunoassay for α-fetoprotein (AFP) in human serum

2008 ◽  
Vol 393 (2) ◽  
pp. 90-94 ◽  
Author(s):  
Xu Wang ◽  
Qian-Yun Zhang ◽  
Zhen-Jia Li ◽  
Xi-Tang Ying ◽  
Jin-Ming Lin
Keyword(s):  
Human Serum ◽  
Enzyme Immunoassay ◽  
High Performance ◽  
Chemiluminescence Enzyme Immunoassay

An efficient method for the synthesis of a polymer brush via click chemistry and its ultrasensitive electrochemical detection of AFP

2018 ◽  
Vol 10 (20) ◽  
pp. 2390-2397 ◽  
Author(s):  
Dongcheng Yang ◽  
Jing Wang ◽  
Hou Chen ◽  
Hui Xu ◽  
Wenxiang Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Click Chemistry ◽  
Tumor Markers ◽  
Electrochemical Detection ◽  
Efficient Method ◽  
Polymer Brush ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection

A sensitive electrochemical immunosensor based on polyacrylonitrile-g-poly(hydroxyethylmethacrylate) (PAN-g-PHEMA)/graphene oxide (GO) composites was prepared for the ultrasensitive detection of tumor markers.

A highly selective colorimetric assay for the determination of creatinine in biological samples using gluconic acid capped silver nanoparticles after ionic liquid based dispersive liquid phase microextraction

2021 ◽  
pp. 1-8
Author(s):  
Susan Sadeghi ◽  
Mohadeseh Hosseinpour-Zaryabi
Keyword(s):  
Silver Nanoparticles ◽  
Ionic Liquid ◽  
Liquid Phase ◽  
Human Serum ◽  
Gluconic Acid ◽  
Ag Nps ◽  
Liquid Phase Microextraction ◽  
Relative Standard ◽  
Serum And Urine

A dispersive liquid-phase microextraction method combined with UV–vis spectrophotometry was utilized to highly selective determination of creatinine in human serum and urine samples. To overcome the interferences in complex matrices, creatinine reacted with 1,4-naphthoquinone-2- potassium sulfonate reagent to produce a red coloured product that could be extracted into a small volume of 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIM]PF6) ionic liquid solvent. To increase the sensitivity of the assay, gluconic acid capped silver nanoparticles (Ag NPs) were used. On addition of Ag NPs to the red coloured extracted product, the solution turned to blue accompanied with a red shift in wavelength around 620 nm that could be detected by the naked eye. The effective variables on the determination of creatinine such as concentration of the reagent, amount of formic and hydrochloric acids, type and volume of the extractant, and concentration of Ag NPs were investigated. Under the optimal conditions, the calibration plot was bimodal with linear ranges from 0.1 to 1.5 µg mL−1 and 1.5 to 105 µg mL−1 creatinine with a limit of detection 0.1 µg mL−1. The relative standard deviation for five measurements at 35 µg mL−1 concentration level was 3.8%. The newly developed assay was used for the determination of creatinine in human serum and urine specimens with satisfactory results.

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