Towards validating a method for two-dimensional electrophoresis/silver staining

2005 ◽  
Vol 26 (12) ◽  
pp. 2461-2469 ◽  
Author(s):  
Wolfgang Schlags ◽  
Michael Walther ◽  
Mohammed Masree ◽  
Martin Kratzel ◽  
Christian R. Noe ◽  
...  
Keyword(s):  
Silver Staining ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Quantitation of human leukocyte proteins after silver staining: A study with two-dimensional electrophoresis

1991 ◽  
Vol 12 (7-8) ◽  
pp. 536-543 ◽  
Author(s):  
Carol S. Giometti ◽  
M. Anne Gemmell ◽  
Sandra L. Tollaksen ◽  
John Taylor
Keyword(s):  
Silver Staining ◽  
Human Leukocyte ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis of human plasma apolipoproteins.

1984 ◽  
Vol 30 (12) ◽  
pp. 2084-2092 ◽  
Author(s):  
D L Sprecher ◽  
L Taam ◽  
H B Brewer
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Detailed Analysis ◽  
Silver Staining ◽  
Lipoprotein Metabolism ◽  
Two Dimensional ◽  
Electrophoretic Method ◽  
Two Dimensional Electrophoresis ◽  
Artery Disease ◽  
Dimensional Electrophoresis

Abstract The two-dimensional electrophoretic method with silver staining we describe better resolves plasma apolipoproteins (apo) than any procedure previously described. It can be used to screen for abnormalities in apoA-I, apoA-II, apoA-IV, apoC-II, apoC-III, apoD, apoE, and apoH. In addition, this is the first presentation of apoD and apoH on two-dimensional gels. This electrophoretic method will permit the quantification of plasma apolipoproteins by computer imaging. Such detailed analysis of the plasma apolipoproteins should provide a better understanding of apolipoprotein function and the role these apolipoproteins play in lipoprotein metabolism and the pathophysiology of dyslipoproteinemias and coronary artery disease.

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