On the use of dimensionless parameters in acid-base theory. III. The molar buffer capacity of trivalent protolytes

The article discusses the role of conjugated lactic acid/ lactate anion (LacH/Lac-) and dihydrogenphosphate anion/ hydrogenphosphate anion (H2PO4-/HPO42-) pairs in the formation of the buffer properties of tissue as a factor determining pH. The buffer properties of homogenates of the tissue of adenocarcinoma of the mammary gland and the adjacent tissue were quantitatively characterized by the buffer capacity which was determined by potentiometric titration. The concentrations of acid anions were determined spectrophotometrically. The material was biopsy specimens of mammary gland adenocarcinoma (T1-4, N0-1, M0) and adjacent tissue of 22 patients aged from 33 to 75 years. It was found that the buffer capacity of tumors is in 2.5 times higher than in normal tissue. It was established that for the tumor tissue, the buffer capacity of the LacH/Lac- system is in 3 times higher, and the buffer capacity of the H2PO4-/HPO42-system is in 2.5 times greater than for normal untransformed tissue. Concentrations of lactate anions (1,93 ± 0,50 vs 0,57 ± 0,22; p <0.001) and phosphate anions (2,54 ± 0,39 vs 0,70 ± 0,19; p <0,001) in homogenates of the tumor tissue were significantly higher in tumor tissue in comparison with the adjacent tissue. A strong correlation was found between the concentration of phosphate anions and the buffer capacity for tumor tissue (r = 0,857; p = 0,002) and for adjacent tissue (r = 0,917; p <0,001). The correlation between the concentration of lactate anions and the buffer capacity for tumor tissues can be estimated as average (r = 0,626; p = 0,053), while it is absent for the adjacent tissue (r = 0,494; p = 0,147). The results suggest that the acid-base properties of homogenates of mammary adenocarcinoma tissues are determined by two buffer systems: LacH/Lac- and H2PO4-/HPO42-, while the intracellular acid-base homeostasis of non-transformed tissues is mainly determined by the H2PO4-/HPO42- system.

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Acid-base properties of metals and glasses of Na2O.P2O5-xB2O3 system

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19842355 ◽

1984 ◽

Vol 49 (10) ◽

pp. 2355-2362 ◽

Cited By ~ 2

Author(s):

Juraj Leško ◽

Marie Dorušková ◽

Jan Tržil

Keyword(s):

Boron Oxide ◽

A Priori ◽

Optical Basicity ◽

Galvanic Cell ◽

Acid Base ◽

Tetrahedral Configuration ◽

Relative Basicity ◽

Base Theory ◽

Base Properties

Boron oxide in the Na2O.P2O5-x B2O3 system behaves as a Lux base. Its addition to Na2O.P2O5 brings about transformation of a Co(II) indicator from octahedral to tetrahedral configuration, increase in the optical basicity ΛPb(II), increase in the relative basicity of the melt as determined by means of a galvanic cell, and depolymerization reactions releasing PO43- ions. In the Na2O-B2O3 system free of P2O5, boron oxide behaves as a Lux acid. The amphoretic nature of B2O3 is explained in terms of Lux's acid-base theory extended in analogy with the protolysis theory. The theoretical optical basicity values do not indicate the amphoretic behaviour of B2O3 because in this approach boron oxide is a priori regarded as more acidic than Na2O.P2O5.

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Variable charges of a red soil from different depths: Acid-base buffer capacity and surface complexation model

Applied Clay Science ◽

10.1016/j.clay.2017.08.003 ◽

2018 ◽

Vol 159 ◽

pp. 107-115 ◽

Cited By ~ 10

Author(s):

Ying Wang ◽

Pengfei Cheng ◽

Fangbai Li ◽

Tongxu Liu ◽

Kuan Cheng ◽

...

Keyword(s):

Buffer Capacity ◽

Surface Complexation ◽

Red Soil ◽

Acid Base ◽

Surface Complexation Model ◽

Different Depths

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Influence of training intensity on adaptations in acid/base transport proteins, muscle buffer capacity, and repeated-sprint ability in active men

Journal of Applied Physiology ◽

10.1152/japplphysiol.00630.2016 ◽

2016 ◽

Vol 121 (6) ◽

pp. 1290-1305 ◽

Cited By ~ 15

Author(s):

Cian McGinley ◽

David J. Bishop

Keyword(s):

Lactate Threshold ◽

Buffer Capacity ◽

Transport Protein ◽

Confidence Limits ◽

Training Intensity ◽

Acid Base ◽

Repeated Sprint Ability ◽

Repeated Sprint ◽

Sprint Ability

McGinley C, Bishop DJ. Influence of training intensity on adaptations in acid/base transport proteins, muscle buffer capacity, and repeated-sprint ability in active men. J Appl Physiol 121: 1290–1305, 2016. First published October 14, 2016; doi: 10.1152/japplphysiol.00630.2016 .—This study measured the adaptive response to exercise training for each of the acid-base transport protein families, including providing isoform-specific evidence for the monocarboxylate transporter (MCT)1/4 chaperone protein basigin and for the electrogenic sodium-bicarbonate cotransporter (NBCe)1. We investigated whether 4 wk of work-matched, high-intensity interval training (HIIT), performed either just above the lactate threshold (HIITΔ20; n = 8), or close to peak aerobic power (HIITΔ90; n = 8), influenced adaptations in acid-base transport protein abundance, nonbicarbonate muscle buffer capacity (βmin vitro), and exercise capacity in active men. Training intensity did not discriminate between adaptations for most proteins measured, with abundance of MCT1, sodium/hydrogen exchanger (NHE) 1, NBCe1, carbonic anhydrase (CA) II, and CAXIV increasing after 4 wk, whereas there was little change in CAIII and CAIV abundance. βmin vitro also did not change. However, MCT4 protein content only increased for HIITΔ20 [effect size (ES): 1.06, 90% confidence limits × / ÷ 0.77], whereas basigin protein content only increased for HIITΔ90 (ES: 1.49, × / ÷ 1.42). Repeated-sprint ability (5 × 6-s sprints; 24 s passive rest) improved similarly for both groups. Power at the lactate threshold only improved for HIITΔ20 (ES: 0.49; 90% confidence limits ± 0.38), whereas peak O2 uptake did not change for either group. Detraining was characterized by the loss of adaptations for all of the proteins measured and for repeated-sprint ability 6 wk after removing the stimulus of HIIT. In conclusion, 4 wk of HIIT induced improvements in each of the acid-base transport protein families, but, remarkably, a 40% difference in training intensity did not discriminate between most adaptations.

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