Selective Detection of Dopamine in the Presence of Ascorbic and Uric Acids through its Covalent Immobilization on Gold Mercaptopropionic Acid Self-assembled Monolayer

2016 ◽  
Vol 29 (1) ◽  
pp. 272-279 ◽  
Author(s):  
Reza Karimi Shervedani ◽  
Sonia Bahrani ◽  
Marzieh Samiei Foroushani ◽  
Fariborz Momenbeik
Keyword(s):  
Covalent Immobilization ◽  
Selective Detection ◽  
Self Assembled Monolayer ◽  
Mercaptopropionic Acid ◽  
Self Assembled

Selective detection of estradiol using a molecularly imprinted self-assembled monolayer on gold surface

2009 ◽  
Vol 1 (2) ◽  
pp. 117-121 ◽  
Author(s):  
Eunjoo Kim ◽  
Sung Jun Lee ◽  
Hyun-Chul Kim ◽  
Se Geun Lee ◽  
Tae-Jung Go ◽  
...  
Keyword(s):  
Gold Surface ◽  
Selective Detection ◽  
Self Assembled Monolayer ◽  
Molecularly Imprinted ◽  
Self Assembled

A Novel Technique for Preparation of the Fluorescence Sensor Based on Covalent Immobilization of 1-Aminopyrene

2011 ◽  
Vol 239-242 ◽  
pp. 1442-1447 ◽  
Author(s):  
Shu Zhen Tan ◽  
Pu Ni Zeng ◽  
Zhong Cao ◽  
Jiao Yun Xia ◽  
Wei Li
Keyword(s):  
Optical Sensors ◽  
Au Nanoparticles ◽  
Covalent Immobilization ◽  
Glass Slide ◽  
Self Assembled Monolayer ◽  
Novel Technique ◽  
Ethylcarbodiimide Hydrochloride ◽  
Carboxylic Groups ◽  
Self Assembled ◽  
Indicator Dyes

A novel technique to covalently immobilize indicator dyes with terminal amino groups for preparing optical sensors is investigated. Au nanoparticles are used as bridges and carriers for anchoring indicator dyes on the surface of a quartz glass slide. 1-Aminopyrene (AP) was employed as an example of indicator dyes and covalently immobilized onto the outmost surface of the glass slide. First, the glass slide was functionalized by (3-mercaptopropyl) trimethoxysilane (MPS) to form a thiol-terminated self-assembled monolayer, where Au nanoparticles were strongly anchored via covalent link. Then, 16-mercaptohexadecanoic acid (MHDA) was self-assembled to bring carboxylic groups onto the surfaces of Au nanoparticles. A further activation by using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) converted the carboxylic group into succinimide ester. Finally, the active succinimide ester was reacted with 1-aminopyrene (AP). Thus, AP was covalently immobilized to the glass slide and an AP-immobilized sensor was obtained. The resulting sensor was used to determine rutin based on fluorescence quenching. It showed a linear response toward rutin (R) from 5.0 × 10-7 to 6.0 × 10-4 mol L-1 with a detection limit of 2.0× 10-7 mol L-1. This AP-immobilized sensor has very satisfactory reproducibility, reversibility, rapid response and no dye-leaching.

scholarly journals Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 227
Author(s):  
Marcin Gwiazda ◽  
Sheetal K. Bhardwaj ◽  
Ewa Kijeńska-Gawrońska ◽  
Wojciech Swieszkowski ◽  
Unni Sivasankaran ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Covalent Immobilization ◽  
Electrochemical Immunosensor ◽  
Collagen I ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Self Assembled Monolayer ◽  
Self Assembled

This research presents an electrochemical immunosensor for collagen I detection using a self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) and covalently immobilized half-reduced monoclonal antibody as a receptor; this allowed for the validation of the collagen I concentration through two different independent methods: electrochemically by Electrochemical Impedance Spectroscopy (EIS), and optically by Surface Plasmon Resonance (SPR). The high unique advantage of the proposed sensor is based on the performance of the stable covalent immobilization of the AuNPs and enzymatically reduced half-IgG collagen I antibodies, which ensured their appropriate orientation onto the sensor’s surface, good stability, and sensitivity properties. The detection of collagen type I was performed in a concentration range from 1 to 5 pg/mL. Moreover, SPR was utilized to confirm the immobilization of the monoclonal half-antibodies and sensing of collagen I versus time. Furthermore, EIS experiments revealed a limit of detection (LOD) of 0.38 pg/mL. The selectivity of the performed immunosensor was confirmed by negligible responses for BSA. The performed approach of the immunosensor is a novel, innovative attempt that enables the detection of collagen I with very high sensitivity in the range of pg/mL, which is significantly lower than the commonly used enzyme-linked immunosorbent assay (ELISA).

Acetylcholinesterase Immobilization on 3-Mercaptopropionic Acid Self Assembled Monolayer for Determination of Pesticides

2007 ◽  
Vol 19 (13) ◽  
pp. 1415-1420 ◽  
Author(s):  
Valber A. Pedrosa ◽  
Josiane Caetano ◽  
Sergio A. S. Machado ◽  
Renato S. Freire ◽  
Mauro Bertotti
Keyword(s):  
Self Assembled Monolayer ◽  
Mercaptopropionic Acid ◽  
Self Assembled
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