Chitosan-carbon Nanofiber Modified Single-use Graphite Electrodes Developed for Electrochemical Detection of DNA Hybridization Related to Hepatitis B Virus

2016 ◽  
Vol 28 (10) ◽  
pp. 2514-2521 ◽  
Author(s):  
Ece Eksin ◽  
Arzum Erdem
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Electrochemical Detection ◽  
Dna Hybridization ◽  
Carbon Nanofiber ◽  
Graphite Electrodes ◽  
B Virus ◽  
Single Use

Use of an HBV-DNA hybridization assay in the evaluation of equivocal hepatitis B virus tests in solid organ donors

2000 ◽  
Vol 10 (1) ◽  
pp. 42-46 ◽  
Author(s):  
Ronald Domen ◽  
Belinda Yen-Lieberman ◽  
Kristine Nelson ◽  
Jimmy Chua ◽  
William Sholtis ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Hybridization ◽  
Hbv Dna ◽  
Hybridization Assay ◽  
Solid Organ ◽  
Organ Donors ◽  
B Virus

scholarly journals Hepatitis B virus DNA is enriched in polymorphonuclear leukocytes

Blood ◽  
1985 ◽  
Vol 66 (6) ◽  
pp. 1251-1253 ◽  
Author(s):  
DI Hoar ◽  
T Bowen ◽  
D Matheson ◽  
MC Poon
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Sequences ◽  
Polymorphonuclear Leukocyte ◽  
Dna Hybridization ◽  
Unit Length ◽  
Viral Dna ◽  
B Genome ◽  
B Virus ◽  
Free Monomer

Abstract DNA hybridization and cell separation techniques were used to determine which blood components contained hepatitis B viral DNA sequences. Free monomer-length hepatitis B virus was found in large amounts in the polymorphonuclear leukocyte cell fraction in two of five HBsAG-positive patients. In these two patients, viral DNA sequences were not detected in the plasma or platelet fraction, whereas the mononuclear cell DNA contained small amounts of a 7.2 kb size unintegrated hepatitis B genome. These studies indicate that the major reservoir of unit-length viral DNA in the asymptomatic hepatitis B carriers studied here was in the polymorphonuclear leukocyte fraction. The basis for the presence of the viral DNA within these cells is presently unknown, but may relate to viral replication within, or phagocytosis of virus by, these cells.

scholarly journals Hepatitis B virus DNA is enriched in polymorphonuclear leukocytes

Blood ◽  
1985 ◽  
Vol 66 (6) ◽  
pp. 1251-1253
Author(s):  
DI Hoar ◽  
T Bowen ◽  
D Matheson ◽  
MC Poon
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Sequences ◽  
Polymorphonuclear Leukocyte ◽  
Dna Hybridization ◽  
Unit Length ◽  
Viral Dna ◽  
B Genome ◽  
B Virus ◽  
Free Monomer

DNA hybridization and cell separation techniques were used to determine which blood components contained hepatitis B viral DNA sequences. Free monomer-length hepatitis B virus was found in large amounts in the polymorphonuclear leukocyte cell fraction in two of five HBsAG-positive patients. In these two patients, viral DNA sequences were not detected in the plasma or platelet fraction, whereas the mononuclear cell DNA contained small amounts of a 7.2 kb size unintegrated hepatitis B genome. These studies indicate that the major reservoir of unit-length viral DNA in the asymptomatic hepatitis B carriers studied here was in the polymorphonuclear leukocyte fraction. The basis for the presence of the viral DNA within these cells is presently unknown, but may relate to viral replication within, or phagocytosis of virus by, these cells.

Electrochemical detection of short sequences related to the hepatitis B virus using MB on chitosan-modified CPE

2007 ◽  
Vol 70 (2) ◽  
pp. 245-249 ◽  
Author(s):  
Guo Mandong ◽  
Li Yanqing ◽  
Guo Hongxia ◽  
Wu Xiaoqin ◽  
Fan Lifang
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Electrochemical Detection ◽  
B Virus

Determination of hepatitis B virus surface antigen in serum with a sandwich immunoassay and capillary electrophoresis–electrochemical detection

2014 ◽  
Vol 6 (8) ◽  
pp. 2484-2489 ◽  
Author(s):  
Yu Chen ◽  
Jie Wang ◽  
Zhongming Liu ◽  
Guoquan Wu
Keyword(s):  
Capillary Electrophoresis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Electrochemical Detection ◽  
Surface Antigen ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Detection Technology ◽  
B Virus

We present a novel immunoassay that uses an efficient and specific sandwich-type protocol and sensitive capillary electrophoresis–electrochemical detection technology for the determination of hepatitis B virus surface antigen.

Novel hybridization indicator methylene blue for the electrochemical detection of short DNA sequences related to the hepatitis B virus

2000 ◽  
Vol 422 (2) ◽  
pp. 139-149 ◽  
Author(s):  
Arzum Erdem ◽  
Kagan Kerman ◽  
Burcu Meric ◽  
Ulus Salih Akarca ◽  
Mehmet Ozsoz
Keyword(s):  
Methylene Blue ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Electrochemical Detection ◽  
Dna Sequences ◽  
B Virus

scholarly journals Electrospun carbon nanofiber-based electrochemical biosensor for the detection of hepatitis B virus

2019 ◽  
Vol 9 (4) ◽  
pp. 4022-4026 ◽  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Sequences ◽  
Electrochemical Biosensor ◽  
Carbon Nanofiber ◽  
Volume Ratio ◽  
Complementary Dna ◽  
Electrochemical Dna Biosensor ◽  
Target Dna ◽  
B Virus

The purpose of this work was to design the electrochemical DNA biosensor based on carbon nanofibers (CNFs) for detecting hepatitis B virus (HBV). The CNFs, due to high conductivity and surface-to-volume ratio, was known as an effective material in the electrochemical biosensor. In this work, we directly used electrospun CNF as the electrode. CNF electrode was modified with electropolymerized glutamic acid (Glu). Then, the probe DNA (pDNA) was conjugated to Glu modified CNFs electrode. The surface morphology of CNFs and Glu modified CNFs were characterized via scanning electron microscopy (SEM). Electropolymerized Glu on the surface of CNF electrode was characterized by Fourier transform infrared spectroscopy (FTIR) as well. The cyclic voltammetry (CV) was used to monitor the target DNA (tDNA). The tDNA was quantified at a linear range from 1 × 10-12 to 1 × 10-6 M with a detection limit of 1.58 × 10-12 M. This electrochemical HBV biosensor had good stability, repeatability, and selectivity for distinguishing complementary DNA from non-complementary and mis-matched DNA sequences.

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