Linear and Branched Pyridyl-Oxazole Oligomers: Synthesis and Circular Dichroism Detectable Effect on c-Myc G-Quadruplex Helicity

2015 ◽  
Vol 2016 (1) ◽  
pp. 122-131 ◽  
Author(s):  
Natalia Rizeq ◽  
Savvas N. Georgiades
Keyword(s):  
Circular Dichroism ◽  
Detectable Effect ◽  
G Quadruplex ◽  
Circular Dichroïsm

scholarly journals A first-in-class and a fished out anticancer platinum compound: cis-[PtCl2(NH3)2] and cis-[PtI2(NH3)2] compared for their reactivity towards DNA model systems

2016 ◽  
Vol 45 (20) ◽  
pp. 8587-8600 ◽  
Author(s):  
Domenica Musumeci ◽  
Chiara Platella ◽  
Claudia Riccardi ◽  
Antonello Merlino ◽  
Tiziano Marzo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Circular Dichroism ◽  
Electrospray Mass Spectrometry ◽  
Model Systems ◽  
Platinum Compound ◽  
Visible Spectroscopy ◽  
G Quadruplex ◽  
Uv Visible ◽  
Circular Dichroïsm

The reactivity of cis-PtI2(NH3)2 with selected DNA model systems, in single, double strand or G-quadruplex form, has been studied by circular dichroism, UV-visible spectroscopy and electrospray mass spectrometry using cisplatin as a control.

THE INFLUENCE OF UREA ON G-QUADRUPLEX AND i-MOTIF STRUCTURES IN COMPLEMENTARY DNA SEQUENCES

2020 ◽  
Vol 54 (2 (252)) ◽  
pp. 115-122
Author(s):  
Ye.B. Dalyan ◽  
L.G. Aslanyan ◽  
I.V. Vardanyan
Keyword(s):  
Circular Dichroism ◽  
Dna Sequences ◽  
Thermal Denaturation ◽  
Temperature Scale ◽  
Single Strand ◽  
Structural Transitions ◽  
Telomeric Dna ◽  
Complementary Dna ◽  
G Quadruplex ◽  
Circular Dichroïsm

In the present study, the methods of circular dichroism and UV/Vis spectrophotometry were used to study the influence of urea on the structural transitions i-motif $\leftrightarrows$ unfolded single strand in cytosine-rich ${\text{d[3}^{\prime}\text{-(CCCAAT)}_{3}\text{CCC-5)}^{\prime}]}$ region of telomeric DNA (Tel22C) and G-quadruplex $\leftrightarrows$ unfolded single strand in complementary guanine-rich strand ${\text{d[5}^{\prime}\text{-A(GGGTTA)}_{3}\text{GGG-3}^{\prime}]}$ (Tel22G) at pH 5.5 and 400 mM Na+. Under these conditions, Tel22C and Tel22G were found to form stable i-motif and G-quadruplex structures. It has been shown that urea (0-8 M) destabilizes the i-motif and G-quadruplex structures, but unlike thermal denaturation, it does not destroy the structures completely. The melting processes of G-quadruplex and i-motif are separated in the temperature scale (at any concentration of urea, the melting of the G-quadruplex starts at temperatures where the melting of the i-motifs has already been completed).

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