Specific Recognition of Human Telomeric G-Quadruplex DNA with Small Molecules and the Conformational Analysis by ESI Mass Spectrometry and Circular Dichroism Spectropolarimetry

2007 ◽  
Vol 13 (17) ◽  
pp. 5018-5023 ◽  
Author(s):  
Jiang Zhou ◽  
Gu Yuan
Keyword(s):  
Mass Spectrometry ◽  
Circular Dichroism ◽  
Conformational Analysis ◽  
Small Molecules ◽  
Quadruplex Dna ◽  
Specific Recognition ◽  
G Quadruplex ◽  
Esi Mass Spectrometry ◽  
Circular Dichroïsm

scholarly journals A first-in-class and a fished out anticancer platinum compound: cis-[PtCl2(NH3)2] and cis-[PtI2(NH3)2] compared for their reactivity towards DNA model systems

2016 ◽  
Vol 45 (20) ◽  
pp. 8587-8600 ◽  
Author(s):  
Domenica Musumeci ◽  
Chiara Platella ◽  
Claudia Riccardi ◽  
Antonello Merlino ◽  
Tiziano Marzo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Circular Dichroism ◽  
Electrospray Mass Spectrometry ◽  
Model Systems ◽  
Platinum Compound ◽  
Visible Spectroscopy ◽  
G Quadruplex ◽  
Uv Visible ◽  
Circular Dichroïsm

The reactivity of cis-PtI2(NH3)2 with selected DNA model systems, in single, double strand or G-quadruplex form, has been studied by circular dichroism, UV-visible spectroscopy and electrospray mass spectrometry using cisplatin as a control.

scholarly journals Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies

2021 ◽  
Vol 22 (19) ◽  
pp. 10399
Author(s):  
Joanna Nowak-Karnowska ◽  
Agata Głuszyńska ◽  
Joanna Kosman ◽  
Grażyna Neunert ◽  
Anna Dembska
Keyword(s):  
Circular Dichroism ◽  
Molecular Targets ◽  
Circular Dichroism Spectroscopy ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Circular Dichroïsm

The interactions of G–quadruplexes of different topologies with highly fluorescent 9-methoxyluminarine ligand 9-MeLM were investigated by fluorescence and circular dichroism spectroscopy. The results showed that 9-methoxyluminarine was able to interact and did not destabilize any investigated molecular targets. The studied compound was selectively quenched by parallel c-MYC G-quadruplex DNA, whereas hybrid and antiparallel G4 topology caused only a negligible decrease in the fluorescence of the ligand. A high decrease of fluorescence of the ligand after binding with c-MYC G-quadruplex suggests that this molecule can be used as a selective probe for parallel G-quadruplexes.

scholarly journals DNA G-quadruplexes for native mass spectrometry in potassium: a database of validated structures in electrospray-compatible conditions

2021 ◽  
Author(s):  
Anirban Ghosh ◽  
Eric Largy ◽  
Valérie Gabelica
Keyword(s):  
Mass Spectrometry ◽  
Drug Targets ◽  
Native Mass Spectrometry ◽  
Drug Binding ◽  
Quadruplex Dna ◽  
Dna Structures ◽  
Nmr Structures ◽  
G Quadruplex ◽  
Screening Assays

Abstract G-quadruplex DNA structures have become attractive drug targets, and native mass spectrometry can provide detailed characterization of drug binding stoichiometry and affinity, potentially at high throughput. However, the G-quadruplex DNA polymorphism poses problems for interpreting ligand screening assays. In order to establish standardized MS-based screening assays, we studied 28 sequences with documented NMR structures in (usually ∼100 mM) potassium, and report here their circular dichroism (CD), melting temperature (Tm), NMR spectra and electrospray mass spectra in 1 mM KCl/100 mM trimethylammonium acetate. Based on these results, we make a short-list of sequences that adopt the same structure in the MS assay as reported by NMR, and provide recommendations on using them for MS-based assays. We also built an R-based open-source application to build and consult a database, wherein further sequences can be incorporated in the future. The application handles automatically most of the data processing, and allows generating custom figures and reports. The database is included in the g4dbr package (https://github.com/EricLarG4/g4dbr) and can be explored online (https://ericlarg4.github.io/G4_database.html).

Enantiopure methoxetamine stereoisomers: chiral resolution, conformational analysis, UV-circular dichroism spectroscopy and electronic circular dichroism

2021 ◽  
Author(s):  
Kun Won Lee ◽  
Ahmed H. E. Hassan ◽  
Youngdo Jeong ◽  
Seolmin Yoon ◽  
Seung-Hwan Kim ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Conformational Analysis ◽  
Absolute Configuration ◽  
Circular Dichroism Spectroscopy ◽  
Chiral Resolution ◽  
Treatment Resistant Depression ◽  
Treatment Resistant ◽  
Electronic Circular Dichroism ◽  
Resistant Depression ◽  
Circular Dichroïsm

Enantioseparation and assignment of absolute configuration of methoxetamine (MXE) enantiopure stereoisomers; a promising novel antidepressant for management of treatment-resistant depression.

Conformational analysis of thymidylate synthase from amino acid sequence and circular dichroism

Biochemistry ◽  
1986 ◽  
Vol 25 (21) ◽  
pp. 6650-6655 ◽  
Author(s):  
Parthasarathy Manavalan ◽  
Denice M. Mittelstaedt ◽  
Michael I. Schimerlik ◽  
W. Curtis Johnson
Keyword(s):  
Circular Dichroism ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Conformational Analysis ◽  
Thymidylate Synthase ◽  
Circular Dichroïsm
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