scholarly journals An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of blood plasma intact lipids

2015 ◽  
Vol 117 (10) ◽  
pp. 1540-1549 ◽  
Author(s):  
Michal A. Surma ◽  
Ronny Herzog ◽  
Andrej Vasilj ◽  
Christian Klose ◽  
Nicolas Christinat ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Blood Plasma ◽  
High Throughput ◽  
Shotgun Lipidomics

scholarly journals DNA-Based Herbal Teas’ Authentication: An ITS2 and psbA-trnH Multi-Marker DNA Metabarcoding Approach

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2120
Author(s):  
Jessica Frigerio ◽  
Giulia Agostinetto ◽  
Valerio Mezzasalma ◽  
Fabrizio De De Mattia ◽  
Massimo Labra ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Medicinal Plants ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
The Other ◽  
Plant Component ◽  
Identification Rate ◽  
Dna Metabarcoding ◽  
Therapeutic Properties

Medicinal plants have been widely used in traditional medicine due to their therapeutic properties. Although they are mostly used as herbal infusion and tincture, employment as ingredients of food supplements is increasing. However, fraud and adulteration are widespread issues. In our study, we aimed at evaluating DNA metabarcoding as a tool to identify product composition. In order to accomplish this, we analyzed fifteen commercial products with DNA metabarcoding, using two barcode regions: psbA-trnH and ITS2. Results showed that on average, 70% (44–100) of the declared ingredients have been identified. The ITS2 marker appears to identify more species (n = 60) than psbA-trnH (n = 35), with an ingredients’ identification rate of 52% versus 45%, respectively. Some species are identified only by one marker rather than the other. Additionally, in order to evaluate the quantitative ability of high-throughput sequencing (HTS) to compare the plant component to the corresponding assigned sequences, in the laboratory, we created six mock mixtures of plants starting both from biomass and gDNA. Our analysis also supports the application of DNA metabarcoding for a relative quantitative analysis. These results move towards the application of HTS analysis for studying the composition of herbal teas for medicinal plants’ traceability and quality control.

scholarly journals High-throughput, Quantitative Analysis of Acrolein-derived DNA Adducts in Human Oral Cells by Immunohistochemistry

2012 ◽  
Vol 60 (11) ◽  
pp. 844-853 ◽  
Author(s):  
Emily J. Greenspan ◽  
Hanjoo Lee ◽  
Marcin Dyba ◽  
Jishen Pan ◽  
Kepher Mekambi ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Dna Adducts ◽  
High Throughput ◽  
Oral Cells

scholarly journals Spotsizer: High-throughput quantitative analysis of microbial growth

BioTechniques ◽  
2016 ◽  
Vol 61 (4) ◽  
Author(s):  
Leanne Bischof ◽  
Martin Převorovský ◽  
Charalampos Rallis ◽  
Daniel C. Jeffares ◽  
Yulia Arzhaeva ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Throughput ◽  
Microbial Growth

Microfluidic Pipette Tip for High-Purity and High-Throughput Blood Plasma Separation from Whole Blood

2017 ◽  
Vol 89 (3) ◽  
pp. 1439-1444 ◽  
Author(s):  
Byeongyeon Kim ◽  
Sein Oh ◽  
Dongwon You ◽  
Sungyoung Choi
Keyword(s):  
Blood Plasma ◽  
High Throughput ◽  
High Purity ◽  
Whole Blood ◽  
Plasma Separation ◽  
Pipette Tip ◽  
Blood Plasma Separation
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