scholarly journals Humoral immune response to citrullinated collagen type II determinants in early rheumatoid arthritis

2005 ◽  
Vol 35 (5) ◽  
pp. 1643-1652 ◽  
Author(s):  
Harald Burkhardt ◽  
Bettina Sehnert ◽  
Robert Bockermann ◽  
Åke Engström ◽  
Jochen R. Kalden ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Immune Response ◽  
Early Rheumatoid Arthritis ◽  
Humoral Immune Response ◽  
Collagen Type ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Humoral Immune

scholarly journals Cartilage-specific autoimmunity in rheumatoid arthritis: characterization of a triple helical B cell epitope in the integrin-binding-domain of collagen type II

2001 ◽  
Vol 31 (6) ◽  
pp. 1666-1673 ◽  
Author(s):  
Hans-Georg Kraetsch ◽  
Christine Unger ◽  
Patrik Wernhoff ◽  
Christian Schneider ◽  
Joachim R. Kalden ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
B Cell ◽  
Collagen Type ◽  
Cell Epitope ◽  
Binding Domain ◽  
Type Ii ◽  
Collagen Type Ii ◽  
B Cell Epitope

scholarly journals Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients

2021 ◽  
Vol 10 (21) ◽  
pp. 5153
Author(s):  
Seyedesomaye Jasemi ◽  
Gian Luca Erre ◽  
Maria Luisa Cadoni ◽  
Marco Bo ◽  
Leonardo A. Sechi
Keyword(s):  
Rheumatoid Arthritis ◽  
Immune Response ◽  
Humoral Immune Response ◽  
Humoral Response ◽  
Human Endogenous Retrovirus ◽  
Serum Samples ◽  
Humoral Immune ◽  
Bivariate Correlation ◽  
Microbial Biomarkers ◽  
Significant Difference

Background/Objective: Chronic humoral immune response against multiple microbial antigens may play a crucial role in the etiopathogenesis of rheumatoid arthritis (RA). We aimed to assess the prevalence and magnitude of antibody response against various bacterial and viral immunogen peptides in the sera of RA patients compared with the general population. Methods: Polyclonal IgG antibodies (Abs) specific for peptides derived from Porphyromonas gingivalis (RgpA, Kpg), Aggregatibacter actinomycetemcomitans (LtxA1, LtxA2), Mycobacterium avium subsp. paratuberculosis (MAP4027), Epstein–Barr virus (EBNA1, EBVBOLF), and human endogenous retrovirus (HERV-W env-su) were detected by ELISA in serum samples from 148 consecutive RA patients and 148 sex and age-matched healthy controls (HCs). In addition, the presence of a relationship between the positivity and the titer of antibodies and RA descriptors was explored by bivariate correlation analysis. Results: RA patients exhibit a higher prevalence of humoral immune response against all tested peptides compared to HCs with a statically significant difference for MAP4027 (30.4% vs. 10.1%), BOLF (25.7% vs. 8.1%), RgpA (24.3% vs. 9.4%), HERV W-env (20.3% vs. 9.4%), and EBNA1 (18.9% vs. 9.4%) peptides. Fifty-three (35.8%) out of 148 RA serum and 93 (62.8%) out of 148 HCs were negative for all pathogen-derived peptides. There was a significant correlation between OD values obtained by ELISA test against all peptides (p < 0.0001). We also found an increased titer and prevalence of Abs against LtxA1 and LtxA2 in seropositive vs. seronegative RF (p = 0.019, p = 0.018). Conclusion: This study demonstrates a significantly increased humoral response against multiple pathogens in patients with RA and implies that they could be an important factor in the pathogenesis of the disease. Therefore, the role of each individual pathogen in RA needs to be further investigated.

scholarly journals Cross-Reactivity between the Rheumatoid Arthritis-Associated Motif EQKRAA and Structurally Related Sequences Found inProteus mirabilis

1999 ◽  
Vol 67 (6) ◽  
pp. 2769-2775 ◽  
Author(s):  
Harmale Tiwana ◽  
Clyde Wilson ◽  
Alison Alvarez ◽  
Ramadan Abuknesha ◽  
Sukhvinder Bansal ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Collagen Type ◽  
Molecular Mimicry ◽  
Cross Reactivity ◽  
Mouse Fibroblast ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Negative Bacterium ◽  
Underlying Mechanisms ◽  
Similar Peptide

ABSTRACT Cross-reactivity or molecular mimicry may be one of the underlying mechanisms involved in the etiopathogenesis of rheumatoid arthritis (RA). Antiserum against the RA susceptibility sequence EQKRAA was shown to bind to a similar peptide ESRRAL present in the hemolysin of the gram-negative bacterium Proteus mirabilis, and an anti-ESRRAL serum reacted with EQKRAA. There was no reactivity with either anti-EQKRAA or anti-ESRRAL to a peptide containing the EDERAA sequence which is present in HLA-DRB1∗0402, an allele not associated with RA. Furthermore, the EQKRAA and ESRRAL antisera bound to a mouse fibroblast transfectant cell line (Dap.3) expressing HLA-DRB1∗0401 but not to DRB1∗0402. However, peptide sequences structurally related to the RA susceptibility motif LEIEKDFTTYGEE (P. mirabilisurease), VEIRAEGNRFTY (collagen type II) and DELSPETSPYVKE (collagen type XI) did not bind significantly to cell lines expressing HLA-DRB1∗0401 or HLA-DRB1∗0402 compared to the control peptide YASGASGASGAS. It is suggested here that molecular mimicry between HLA alleles associated with RA and P. mirabilis may be relevant in the etiopathogenesis of the disease.

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