scholarly journals Environmental DNA improves Eastern Hellbender ( Cryptobranchus alleganiensis alleganiensis ) detection over conventional sampling methods

2019 ◽  
Vol 1 (1) ◽  
pp. 86-96 ◽  
Author(s):  
Sean M. Wineland ◽  
Rachel F. Arrick ◽  
Shane M. Welch ◽  
Thomas K. Pauley ◽  
Jennifer J. Mosher ◽  
...  
Keyword(s):  
Sampling Methods ◽  
Environmental Dna ◽  
Cryptobranchus Alleganiensis ◽  
Eastern Hellbender

Using environmental DNA and occupancy modelling to identify drivers of eastern hellbender (Cryptobranchus alleganiensis alleganiensis) extirpation

2018 ◽  
Vol 64 (1) ◽  
pp. 208-221 ◽  
Author(s):  
Sean M. Wineland ◽  
Shane M. Welch ◽  
Thomas K. Pauley ◽  
Joseph J. Apodaca ◽  
Max Olszack ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Occupancy Modelling ◽  
Cryptobranchus Alleganiensis ◽  
Eastern Hellbender

Spatial Ecology of the Eastern Hellbender (Cryptobranchus alleganiensis alleganiensis) in Indiana

Herpetologica ◽  
2011 ◽  
Vol 67 (2) ◽  
pp. 135-145 ◽  
Author(s):  
Nicholas G Burgmeier ◽  
Trent M Sutton ◽  
Rod N Williams
Keyword(s):  
Spatial Ecology ◽  
Cryptobranchus Alleganiensis ◽  
Eastern Hellbender

Observations on habitat preference of juvenile eastern hellbender salamanders (Cryptobranchus alleganiensis)

2020 ◽  
Vol 23 (2) ◽  
pp. 119-124
Author(s):  
Shem Unger ◽  
Catherine Bodinof-Jachowski ◽  
Lauren Diaz ◽  
Lori A. Williams
Keyword(s):  
Habitat Preference ◽  
Cryptobranchus Alleganiensis ◽  
Eastern Hellbender

Population Status of the Eastern Hellbender (Cryptobranchus alleganiensis alleganiensis) in Indiana

2011 ◽  
Vol 45 (2) ◽  
pp. 195-201 ◽  
Author(s):  
Nicholas G. Burgmeier ◽  
Shem D. Unger ◽  
Trent M. Sutton ◽  
Rod N. Williams
Keyword(s):  
Population Status ◽  
Cryptobranchus Alleganiensis ◽  
Eastern Hellbender

scholarly journals eDNAir: proof of concept that animal DNA can be collected from air sampling

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11030
Author(s):  
Elizabeth L. Clare ◽  
Chloe K. Economou ◽  
Chris G. Faulkes ◽  
James D. Gilbert ◽  
Frances Bennett ◽  
...  
Keyword(s):  
Sampling Methods ◽  
Air Sampling ◽  
Research Programme ◽  
Environmental Dna ◽  
Aquatic Systems ◽  
Ecological Research ◽  
Proof Of Concept ◽  
Confined Space ◽  
Substantial Interest

Environmental DNA (eDNA) is one of the fastest developing tools for species biomonitoring and ecological research. However, despite substantial interest from research, commercial and regulatory sectors, it has remained primarily a tool for aquatic systems with a small amount of work in substances such as soil, snow and rain. Here we demonstrate that eDNA can be collected from air and used to identify mammals. Our proof of concept successfully demonstrated that eDNA sampled from air contained mixed templates which reflect the species known to be present within a confined space and that this material can be accessed using existing sampling methods. We anticipate this demonstration will initiate a much larger research programme in terrestrial airDNA sampling and that this may rapidly advance biomonitoring approaches. Lastly, we outline these and potential related applications we expect to benefit from this development.

An eDNA approach to detect eastern hellbenders (Cryptobranchus a. alleganiensis) using samples of water

2012 ◽  
Vol 39 (7) ◽  
pp. 629 ◽  
Author(s):  
Zachary H. Olson ◽  
Jeffrey T. Briggler ◽  
Rod N. Williams
Keyword(s):  
Water Samples ◽  
Effective Means ◽  
Environmental Dna ◽  
Cost Effective ◽  
Negative Control ◽  
Specificity And Sensitivity ◽  
Genetic Sampling ◽  
Conservation Concern ◽  
Species Specific ◽  
Eastern Hellbender

Context Environmental DNA, or eDNA, methods are a novel application of non-invasive genetic sampling in which DNA from organisms is detected via sampling of water or soil, typically for the purposes of determining the presence or absence of an organism. eDNA methods have the potential to revolutionise the study of rare or endangered taxa. Aims We evaluated the efficacy of eDNA sampling to detect populations of an amphibian of conservation concern, the eastern hellbender (Cryptobranchus a. alleganiensis), indirectly from their aquatic environments. Methods We developed species-specific primers, validated their specificity and sensitivity, and assessed the utility of our methods in silico and in laboratory trials. In the field, we collected water samples from three sites with known densities of hellbenders, and from one site where hellbenders do not occur. We filtered water samples, extracted DNA from filters, and assayed the extraction products for hellbender DNA by using polymerase chain reaction (PCR) and gel electrophoresis. Key results Our methods detected hellbenders at densities approaching the lowest of reported natural densities. The low-density site (0.16 hellbenders per 100 m2) yielded two positive amplifications, the medium-density site (0.38 hellbenders per 100 m2) yielded eight positive amplifications, and the high-density site (0.88 hellbenders per 100 m2) yielded 10 positive amplifications. The apparent relationship between density and detection was obfuscated when river discharge was considered. There was no amplification in any negative control. Conclusion eDNA methods may represent a cost-effective means by which to establish broad-scale patterns of occupancy for hellbenders. Implications eDNA can be considered a valuable tool for detecting many species that are otherwise difficult to study.

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