scholarly journals Utility of environmental DNA analysis for effective monitoring of invasive fish species in reservoirs

Ecosphere ◽  
2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Toshiaki Jo ◽  
Saki Ikeda ◽  
Arisa Fukuoka ◽  
Takashi Inagawa ◽  
Jiro Okitsu ◽  
...  
Keyword(s):  
Fish Species ◽  
Dna Analysis ◽  
Environmental Dna ◽  
Invasive Fish

scholarly journals Application of environmental DNA analysis to inform invasive fish eradication operations

2017 ◽  
Vol 104 (3-4) ◽  
Author(s):  
Phillip I. Davison ◽  
Gordon H. Copp ◽  
Véronique Créach ◽  
Lorenzo Vilizzi ◽  
J. R. Britton
Keyword(s):  
Dna Analysis ◽  
Environmental Dna ◽  
Invasive Fish

scholarly journals Environmental DNA detection of rare and invasive fish species in two Great Lakes tributaries

2017 ◽  
Vol 27 (1) ◽  
pp. 112-127 ◽  
Author(s):  
Katherine D. Balasingham ◽  
Ryan P. Walter ◽  
Nicholas E. Mandrak ◽  
Daniel D. Heath
Keyword(s):  
Great Lakes ◽  
Fish Species ◽  
Dna Detection ◽  
Environmental Dna ◽  
Invasive Fish

scholarly journals Using Environmental DNA to Estimate the Distribution of an Invasive Fish Species in Ponds

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56584 ◽  
Author(s):  
Teruhiko Takahara ◽  
Toshifumi Minamoto ◽  
Hideyuki Doi
Keyword(s):  
Fish Species ◽  
Environmental Dna ◽  
Invasive Fish

scholarly journals Simultaneous absolute quantification and sequencing of fish environmental DNA in a mesocosm by quantitative sequencing technique

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuhiko Hoshino ◽  
Ryohei Nakao ◽  
Hideyuki Doi ◽  
Toshifumi Minamoto
Keyword(s):  
Fish Species ◽  
High Throughput Sequencing ◽  
Correlation Coefficients ◽  
Environmental Dna ◽  
Digital Pcr ◽  
Absolute Quantification ◽  
Taqman Probe ◽  
Individual Species ◽  
Natural Environments ◽  
Target Sequence

AbstractThe combination of high-throughput sequencing technology and environmental DNA (eDNA) analysis has the potential to be a powerful tool for comprehensive, non-invasive monitoring of species in the environment. To understand the correlation between the abundance of eDNA and that of species in natural environments, we have to obtain quantitative eDNA data, usually via individual assays for each species. The recently developed quantitative sequencing (qSeq) technique enables simultaneous phylogenetic identification and quantification of individual species by counting random tags added to the 5′ end of the target sequence during the first DNA synthesis. Here, we applied qSeq to eDNA analysis to test its effectiveness in biodiversity monitoring. eDNA was extracted from water samples taken over 4 days from aquaria containing five fish species (Hemigrammocypris neglectus, Candidia temminckii, Oryzias latipes, Rhinogobius flumineus, and Misgurnus anguillicaudatus), and quantified by qSeq and microfluidic digital PCR (dPCR) using a TaqMan probe. The eDNA abundance quantified by qSeq was consistent with that quantified by dPCR for each fish species at each sampling time. The correlation coefficients between qSeq and dPCR were 0.643, 0.859, and 0.786 for H. neglectus, O. latipes, and M. anguillicaudatus, respectively, indicating that qSeq accurately quantifies fish eDNA.

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