scholarly journals A simple, rapid method for detecting seven common invasive fish species in Europe from environmental DNA

2018 ◽  
Vol 28 (3) ◽  
pp. 619-629 ◽  
Author(s):  
Laura Clusa ◽  
E. García-Vázquez
Ecosphere ◽  
2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Toshiaki Jo ◽  
Saki Ikeda ◽  
Arisa Fukuoka ◽  
Takashi Inagawa ◽  
Jiro Okitsu ◽  
...  

2017 ◽  
Vol 27 (1) ◽  
pp. 112-127 ◽  
Author(s):  
Katherine D. Balasingham ◽  
Ryan P. Walter ◽  
Nicholas E. Mandrak ◽  
Daniel D. Heath

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56584 ◽  
Author(s):  
Teruhiko Takahara ◽  
Toshifumi Minamoto ◽  
Hideyuki Doi

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuhiko Hoshino ◽  
Ryohei Nakao ◽  
Hideyuki Doi ◽  
Toshifumi Minamoto

AbstractThe combination of high-throughput sequencing technology and environmental DNA (eDNA) analysis has the potential to be a powerful tool for comprehensive, non-invasive monitoring of species in the environment. To understand the correlation between the abundance of eDNA and that of species in natural environments, we have to obtain quantitative eDNA data, usually via individual assays for each species. The recently developed quantitative sequencing (qSeq) technique enables simultaneous phylogenetic identification and quantification of individual species by counting random tags added to the 5′ end of the target sequence during the first DNA synthesis. Here, we applied qSeq to eDNA analysis to test its effectiveness in biodiversity monitoring. eDNA was extracted from water samples taken over 4 days from aquaria containing five fish species (Hemigrammocypris neglectus, Candidia temminckii, Oryzias latipes, Rhinogobius flumineus, and Misgurnus anguillicaudatus), and quantified by qSeq and microfluidic digital PCR (dPCR) using a TaqMan probe. The eDNA abundance quantified by qSeq was consistent with that quantified by dPCR for each fish species at each sampling time. The correlation coefficients between qSeq and dPCR were 0.643, 0.859, and 0.786 for H. neglectus, O. latipes, and M. anguillicaudatus, respectively, indicating that qSeq accurately quantifies fish eDNA.


2021 ◽  
Vol 33 (2) ◽  
pp. 84-94
Author(s):  
Seung-Chul Park ◽  
Kwang Yeol Lee ◽  
Kwang-Seek Choi ◽  
Mee-Sook Han ◽  
Myeong-Hun Ko

Fisheries ◽  
2021 ◽  
Vol 2021 (1) ◽  
pp. 52-60
Author(s):  
Andrey Bykov

Based on the results of comprehensive fisheries research, a brief description of the ecosystem of the lake of the Shaturskaya group operated in the mode of the cooling reservoir of the Shaturskaya GRES is given. The features of the thermal regime of the Shatursky lakes in the zone of the circulating flow of cooled waters are considered. A brief description of the species composition and quantitative indicators of the development of phytoplankton, zooplankton and macrozoobenthos communities is given. The structure of fish catches in the lakes depending on the fishing gear used is considered according to the data of accounting surveys with set nets and fry drag. The process of formation of the ichthyofauna of Shatursky lakes, characteristic of natural and man-made ecosystems of reservoirs-coolers of energy facilities, is shown. The occurrence of native and invasive fish species in catches is described, and the mechanism of seasonal migration of fish depending on the temperature and oxygen regimes of the Shatursky lakes is considered.


Sign in / Sign up

Export Citation Format

Share Document