Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium

2014 ◽  
Vol 97 (11) ◽  
pp. 65-73 ◽  
Author(s):  
Norimitsu Takamura ◽  
Douyan Wang ◽  
Takao Satoh ◽  
Takao Namihira ◽  
Hisato Saitoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Plasma Jet ◽  
Helium Plasma ◽  
Cell Culture Medium

Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium

2013 ◽  
Vol 133 (5) ◽  
pp. 278-285
Author(s):  
Norimitsu Takamura ◽  
Douyan Wang ◽  
Takao Satoh ◽  
Takao Namihira ◽  
Hisato Saitoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Plasma Jet ◽  
Helium Plasma ◽  
Cell Culture Medium

Production of Reactive Oxygen and Nitrogen Species in a Cell Culture Medium Exposed to an Atmospheric Pressure Plasma Jet

MRS Advances ◽  
2017 ◽  
Vol 2 (18) ◽  
pp. 987-993 ◽  
Author(s):  
Hirofumi Kurita ◽  
Junichiro Miyamoto ◽  
Saki Miyachika ◽  
Yoshito Uchihashi ◽  
Hachiro Yasuda ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Liquid Surface ◽  
Reactive Oxygen ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Cell Culture Medium ◽  
Nitrogen Species ◽  
Pressure Plasma

ABSTRACTCold atmospheric pressure plasma has been intensively studied due to growing interest in biomedical applications. For example, it has been revealed that the plasma-irradiated cell culture medium (PAM: Plasma Activated Medium) shows antitumor effect as well as the direct plasma treatment. Long-lived reactive oxygen and nitrogen species (RONS), such as hydrogen peroxide (H2O2), nitrite (NO2-), and nitrate (NO3-), are considered as a main product in PAM. Therefore, investigation of the production characteristics of RONS in PAM with different experimental parameters is important. Here, we focused on humidification of feed gas (argon or helium) and effect of gap between the nozzle of the plasma jet and liquid surface. As a result, H2O2 concentration was increased by humidification of both feed gases. However, the concentration of NO2- and NO3- was decreased by the humidification of helium. In addition, the remarkable effect of the humidification on H2O2 production was observed when the plasma jet was in contact with the liquid surface.

Reduced HeLa cell viability in methionine-containing cell culture medium irradiated with microwave-excited atmospheric-pressure plasma

2018 ◽  
Vol 15 (3) ◽  
pp. 1700200 ◽  
Author(s):  
Yohei Takahashi ◽  
Yusuke Taki ◽  
Keigo Takeda ◽  
Hiroshi Hashizume ◽  
Hiromasa Tanaka ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hela Cell ◽  
Cell Viability ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Atmospheric Pressure Plasma ◽  
Cell Culture Medium ◽  
Pressure Plasma

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

2020 ◽  
Author(s):  
Federica Saponaro ◽  
Marco Borsò ◽  
Sara Verlotta ◽  
Lavinia Bandini ◽  
Alessandro Saba ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Low Concentration

scholarly journals Defined MSC exosome with high yield and purity to improve regenerative activity

2021 ◽  
Vol 12 ◽  
pp. 204173142110086
Author(s):  
Jun Yong Kim ◽  
Won-Kyu Rhim ◽  
Yong-In Yoo ◽  
Da-Seul Kim ◽  
Kyoung-Won Ko ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Density Lipoprotein ◽  
High Yield ◽  
Human Umbilical Cord ◽  
Cell Culture Medium ◽  
Human Coronary Artery ◽  
Isolation Methods ◽  
Regenerative Activity ◽  
Yield And Purity

Exosomes derived from mesenchymal stem cells (MSCs) have been studied as vital components of regenerative medicine. Typically, various isolation methods of exosomes from cell culture medium have been developed to increase the isolation yield of exosomes. Moreover, the exosome-depletion process of serum has been considered to result in clinically active and highly purified exosomes from the cell culture medium. Our aim was to compare isolation methods, ultracentrifuge (UC)-based conventional method, and tangential flow filtration (TFF) system-based method for separation with high yield, and the bioactivity of the exosome according to the purity of MSC-derived exosome was determined by the ratio of Fetal bovine serum (FBS)-derived exosome to MSC-derived exosome depending on exosome depletion processes of FBS. The TFF-based isolation yield of exosome derived from human umbilical cord MSC (UCMSC) increased two orders (92.5 times) compared to UC-based isolation method. Moreover, by optimizing the process of depleting FBS-derived exosome, the purity of UCMSC-derived exosome, evaluated using the expression level of MSC exosome surface marker (CD73), was about 15.6 times enhanced and the concentration of low-density lipoprotein-cholesterol (LDL-c), known as impurities resulting from FBS, proved to be negligibly detected. The wound healing and angiogenic effects of highly purified UCMSC-derived exosomes were improved about 23.1% and 71.4%, respectively, with human coronary artery endothelial cells (HCAEC). It suggests that the defined MSC exosome with high yield and purity could increase regenerative activity.

scholarly journals Optical diagnostics of the characteristics of a square unipolar nanosecond pulse-driven atmospheric pressure helium plasma jet

AIP Advances ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 125218
Author(s):  
T. Y. Tang ◽  
H. S. Kim ◽  
G. H. Kim ◽  
B. Lee ◽  
H. J. Lee
Keyword(s):  
Atmospheric Pressure ◽  
Plasma Jet ◽  
Optical Diagnostics ◽  
Nanosecond Pulse ◽  
Helium Plasma
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