Reduced HeLa cell viability in methionine-containing cell culture medium irradiated with microwave-excited atmospheric-pressure plasma

2018 ◽  
Vol 15 (3) ◽  
pp. 1700200 ◽  
Author(s):  
Yohei Takahashi ◽  
Yusuke Taki ◽  
Keigo Takeda ◽  
Hiroshi Hashizume ◽  
Hiromasa Tanaka ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hela Cell ◽  
Cell Viability ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Atmospheric Pressure Plasma ◽  
Cell Culture Medium ◽  
Pressure Plasma

Production of Reactive Oxygen and Nitrogen Species in a Cell Culture Medium Exposed to an Atmospheric Pressure Plasma Jet

MRS Advances ◽  
2017 ◽  
Vol 2 (18) ◽  
pp. 987-993 ◽  
Author(s):  
Hirofumi Kurita ◽  
Junichiro Miyamoto ◽  
Saki Miyachika ◽  
Yoshito Uchihashi ◽  
Hachiro Yasuda ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Liquid Surface ◽  
Reactive Oxygen ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Cell Culture Medium ◽  
Nitrogen Species ◽  
Pressure Plasma

ABSTRACTCold atmospheric pressure plasma has been intensively studied due to growing interest in biomedical applications. For example, it has been revealed that the plasma-irradiated cell culture medium (PAM: Plasma Activated Medium) shows antitumor effect as well as the direct plasma treatment. Long-lived reactive oxygen and nitrogen species (RONS), such as hydrogen peroxide (H2O2), nitrite (NO2-), and nitrate (NO3-), are considered as a main product in PAM. Therefore, investigation of the production characteristics of RONS in PAM with different experimental parameters is important. Here, we focused on humidification of feed gas (argon or helium) and effect of gap between the nozzle of the plasma jet and liquid surface. As a result, H2O2 concentration was increased by humidification of both feed gases. However, the concentration of NO2- and NO3- was decreased by the humidification of helium. In addition, the remarkable effect of the humidification on H2O2 production was observed when the plasma jet was in contact with the liquid surface.

Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium

2013 ◽  
Vol 133 (5) ◽  
pp. 278-285
Author(s):  
Norimitsu Takamura ◽  
Douyan Wang ◽  
Takao Satoh ◽  
Takao Namihira ◽  
Hisato Saitoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Plasma Jet ◽  
Helium Plasma ◽  
Cell Culture Medium

Synthesis of calcium oxalate crystals in culture medium irradiated with non-equilibrium atmospheric-pressure plasma

2016 ◽  
Vol 9 (9) ◽  
pp. 096201 ◽  
Author(s):  
Naoyuki Kurake ◽  
Hiromasa Tanaka ◽  
Kenji Ishikawa ◽  
Kae Nakamura ◽  
Hiroaki Kajiyama ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Atmospheric Pressure Plasma ◽  
Calcium Oxalate Crystals ◽  
Pressure Plasma ◽  
Oxalate Crystals ◽  
Non Equilibrium

Atmospheric Pressure Plasma Polymer of Ethyl Lactate: In Vitro Degradation and Cell Viability Studies

2016 ◽  
Vol 13 (7) ◽  
pp. 711-721 ◽  
Author(s):  
Morgane Laurent ◽  
Julia Koehler ◽  
Gad Sabbatier ◽  
Corinne A. Hoesli ◽  
Nicolas Gherardi ◽  
...  
Keyword(s):  
Cell Viability ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Polymer ◽  
Ethyl Lactate ◽  
In Vitro Degradation ◽  
Pressure Plasma
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