scholarly journals Melanic pigmentation and light preference within and between two Drosophila species

2021 ◽  
Author(s):  
Arielle M. Cooley ◽  
Suzanne Schmitz ◽  
Eduardo J. Cabrera ◽  
Mitchell Cutter ◽  
Maxwell Sheffield ◽  
...  
Keyword(s):  
Drosophila Species ◽  
Light Preference

Niche Separation in a Pair of Homosequential Drosophila Species from the Island of Hawaii

1973 ◽  
Vol 107 (958) ◽  
pp. 766-774 ◽  
Author(s):  
K. Y. Kaneshiro ◽  
H. L. Carson ◽  
F. E. Clayton ◽  
W. B. Heed
Keyword(s):  
Drosophila Species ◽  
Niche Separation

Specialization and performance trade‐offs across hosts in cactophilic Drosophila species

2021 ◽  
Author(s):  
Santiago Bouzas ◽  
María F. Barbarich ◽  
Eduardo M. Soto ◽  
Julián Padró ◽  
Valeria P. Carreira ◽  
...  
Keyword(s):  
Drosophila Species ◽  
Cactophilic Drosophila ◽  
Trade Offs ◽  
And Performance

Divergent metabolomic profiles of cold-exposed mature and immature females of tropical versus temperate Drosophila species

2021 ◽  
pp. 110995
Author(s):  
Julián Mensch ◽  
Lucas Kreiman ◽  
Pablo E. Schilman ◽  
Esteban Hasson ◽  
David Renault ◽  
...  
Keyword(s):  
Drosophila Species

scholarly journals RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species

2021 ◽  
Vol 4 (1) ◽  
pp. 20
Author(s):  
Mujeeb Shittu ◽  
Tessa Steenwinkel ◽  
William Dion ◽  
Nathan Ostlund ◽  
Komal Raja ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Expression Patterns ◽  
Drosophila Species ◽  
Gene Expression Patterns ◽  
Probe Design ◽  
Tissue Preparation ◽  
Design And Synthesis ◽  
Rna In Situ Hybridization

RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.

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