Niche Separation in a Pair of Homosequential Drosophila Species from the Island of Hawaii

1973 ◽  
Vol 107 (958) ◽  
pp. 766-774 ◽  
Author(s):  
K. Y. Kaneshiro ◽  
H. L. Carson ◽  
F. E. Clayton ◽  
W. B. Heed
Keyword(s):  
Drosophila Species ◽  
Niche Separation

Specialization and performance trade‐offs across hosts in cactophilic Drosophila species

2021 ◽  
Author(s):  
Santiago Bouzas ◽  
María F. Barbarich ◽  
Eduardo M. Soto ◽  
Julián Padró ◽  
Valeria P. Carreira ◽  
...  
Keyword(s):  
Drosophila Species ◽  
Cactophilic Drosophila ◽  
Trade Offs ◽  
And Performance

Divergent metabolomic profiles of cold-exposed mature and immature females of tropical versus temperate Drosophila species

2021 ◽  
pp. 110995
Author(s):  
Julián Mensch ◽  
Lucas Kreiman ◽  
Pablo E. Schilman ◽  
Esteban Hasson ◽  
David Renault ◽  
...  
Keyword(s):  
Drosophila Species

scholarly journals RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species

2021 ◽  
Vol 4 (1) ◽  
pp. 20
Author(s):  
Mujeeb Shittu ◽  
Tessa Steenwinkel ◽  
William Dion ◽  
Nathan Ostlund ◽  
Komal Raja ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Expression Patterns ◽  
Drosophila Species ◽  
Gene Expression Patterns ◽  
Probe Design ◽  
Tissue Preparation ◽  
Design And Synthesis ◽  
Rna In Situ Hybridization

RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.

scholarly journals Marine ammonia-oxidising archaea and bacteria occupy distinct iron and copper niches

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Roxana T. Shafiee ◽  
Poppy J. Diver ◽  
Joseph T. Snow ◽  
Qiong Zhang ◽  
Rosalind E. M. Rickaby
Keyword(s):  
Comparative Genomics ◽  
Trace Metals ◽  
Trace Metal ◽  
Ammonia Oxidation ◽  
Testable Hypothesis ◽  
Niche Separation ◽  
Toxicity Threshold ◽  
Geologic Time ◽  
Additional Explanation ◽  
Ammonia Oxidising Archaea

AbstractAmmonia oxidation by archaea and bacteria (AOA and AOB), is the first step of nitrification in the oceans. As AOA have an ammonium affinity 200-fold higher than AOB isolates, the chemical niche allowing AOB to persist in the oligotrophic ocean remains unclear. Here we show that marine isolates, Nitrosopumilus maritimus strain SCM1 (AOA) and Nitrosococcus oceani strain C-107 (AOB) have contrasting physiologies in response to the trace metals iron (Fe) and copper (Cu), holding potential implications for their niche separation in the oceans. A greater affinity for unchelated Fe may allow AOB to inhabit shallower, euphotic waters where ammonium supply is high, but competition for Fe is rife. In contrast to AOB, AOA isolates have a greater affinity and toxicity threshold for unchelated Cu providing additional explanation to the greater success of AOA in the marine environment where Cu availability can be highly variable. Using comparative genomics, we predict that the proteomic and metal transport basis giving rise to contrasting physiologies in isolates is widespread across phylogenetically diverse marine AOA and AOB that are not yet available in pure culture. Our results develop the testable hypothesis that ammonia oxidation may be limited by Cu in large tracts of the open ocean and suggest a relatively earlier emergence of AOB than AOA when considered in the context of evolving trace metal availabilities over geologic time.

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