scholarly journals Attracting Common Carp to a bait site with food reveals strong positive relationships between fish density, feeding activity, environmental DNA, and sex pheromone release that could be used in invasive fish management

2018 ◽  
Vol 8 (13) ◽  
pp. 6714-6727 ◽  
Author(s):  
Ratna Ghosal ◽  
Jessica J. Eichmiller ◽  
Bruce A. Witthuhn ◽  
Peter W. Sorensen
2019 ◽  
Vol 46 (1) ◽  
pp. 10-20 ◽  
Author(s):  
Stephen P. Foster ◽  
Karin G. Anderson ◽  
Jérôme Casas

2021 ◽  
Author(s):  
Sarah Lok Ting Kwong ◽  
Cecilia Villacorta-Rath ◽  
Jason Doyle ◽  
Sven Uthicke

Abstract Population outbreaks of the corallivorous crown-of-thorns seastar (CoTS; Acanthaster spp.) are significant threats to the Indo-Pacific reefs. Although recent research demonstrated that environmental DNA (eDNA) techniques could improve CoTS monitoring and management, the interpretation of surveillance results has been limited by uncertainties about eDNA dynamics in aquatic environments. Here, we aimed to identify biotic and abiotic factors affecting the shedding and degradation rates of CoTS eDNA. Using laboratory-raised 8-month-old juvenile CoTS, aquarium experiments were conducted to test the effect of two temperatures (24 and 28°C) and three feeding treatments (no food, food available and food inaccessible) on eDNA shedding rate. Additionally, CoTS eDNA degradation rate was quantified under three temperatures (24, 26 and 28°C). We found that eDNA shedding rate was affected by feeding treatment (p < 0.0001) but not temperature. Specifically, the shedding rate under food accessible treatment was about 7 times higher than that of food inaccessible treatment (p < 0.0001), whereas the presence of coral reduced the shedding rate by half (food inaccessible vs no food, p = 0.0249). Degradation of CoTS eDNA was rapid (half-life = 14h) and not affected by temperature. Our results demonstrated that feeding activity increased eDNA release, but some of the released DNA was lost potentially due to binding to coral surface mucus layer or skeleton. The rapid degradation rate indicated that results of eDNA surveillance likely reflects recent and local occurrence of CoTS. Although further testing is needed, this study provided support for using eDNA as a novel detection tool for early life stages of CoTS on coral reefs.


1996 ◽  
Vol 25 (2) ◽  
pp. 446-451 ◽  
Author(s):  
Harry H. Shorey ◽  
Curtis B. Sisk ◽  
Roland G. Gerber

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