Beyond DNA puffs: What can we learn from studying sciarids?

genesis ◽  
2016 ◽  
Vol 54 (7) ◽  
pp. 361-378 ◽  
Author(s):  
Claudio Roberto Simon ◽  
Fábio Siviero ◽  
Nadia Monesi
Keyword(s):  
1993 ◽  
Vol 160 (1) ◽  
pp. 73-84 ◽  
Author(s):  
Nan Wu ◽  
Chun Liang ◽  
Susan M. Dibartolomeis ◽  
Heidi S. Smith ◽  
Susan A. Gerbi

1977 ◽  
Vol 23 (11-12) ◽  
pp. 1455-1459 ◽  
Author(s):  
C.E. Winter ◽  
A.G. de Bianchi ◽  
W.R. Terra ◽  
F.J.S. Lara

2008 ◽  
Vol 16 (4) ◽  
pp. 609-622 ◽  
Author(s):  
Juliana Aparecida Candido-Silva ◽  
Daniela Palermo de Carvalho ◽  
Graciana Rodrigues Coelho ◽  
Jorge Cury de Almeida

1991 ◽  
Vol 2 (11) ◽  
pp. 875-888 ◽  
Author(s):  
B Bienz-Tadmor ◽  
H S Smith ◽  
S A Gerbi

DNA puffs occur in Sciarid salivary gland chromosomes; they are sites of DNA amplification and intense transcription and they appear to encode secreted structural proteins needed for pupation. In this report we have used P-element transformation of Drosophila to study regulation of a Sciara DNA puff gene. We found that a 718-bp promoter fragment of DNA puff gene II/9-1 from Sciara coprophila directs expression of the bacterial reporter gene CAT in late prepupal salivary glands of transgenic Drosophila melanogaster. The identical tissue and analogous stage specificity indicate that some aspects of the ecdysone response are evolutionarily conserved between Drosophila and Sciara. When transgenic salivary glands are cultured in vitro, CAT activity is rapidly induced by ecdysone, suggesting direct control of gene expression by the ecdysone receptor. Putative stage-specific factors limit expression of the chimeric Sciara-CAT gene in transgenic Drosophila to late prepupae but not to third instar larvae when ecdysone titers are also high.


Chromosoma ◽  
1984 ◽  
Vol 89 (4) ◽  
pp. 280-284 ◽  
Author(s):  
Eduardo M. Laicine ◽  
Maurílio A. R. Alves ◽  
Jorge C. de Almeida ◽  
Elizete Rizzo ◽  
Wander C. Albernaz ◽  
...  

2013 ◽  
Vol 21 (4) ◽  
pp. 345-360 ◽  
Author(s):  
Gerald M. Liew ◽  
Michael S. Foulk ◽  
Susan A. Gerbi

1976 ◽  
Vol 110 (4-5) ◽  
pp. 337-346 ◽  
Author(s):  
Laura M. C. Forino ◽  
Roberto Cremonini ◽  
Anna M. Tagliasacchi

Nature ◽  
1969 ◽  
Vol 222 (5193) ◽  
pp. 605-605 ◽  
Author(s):  
REBA MIRSKY GOODMAN
Keyword(s):  

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