Cyclin E/Cdk2 is required for sperm maturation, but not DNA replication, in early sea urchin embryos

genesis ◽  
2007 ◽  
Vol 45 (5) ◽  
pp. 282-291 ◽  
Author(s):  
Bradley J. Schnackenberg ◽  
Robert E. Palazzo ◽  
William F. Marzluff
Keyword(s):  
Dna Replication ◽  
Sea Urchin ◽  
Cyclin E ◽  
Sperm Maturation ◽  
Sea Urchin Embryos

scholarly journals Novel localization and possible functions of cyclin E in early sea urchin development

2002 ◽  
Vol 115 (1) ◽  
pp. 113-121 ◽  
Author(s):  
Bradley J. Schnackenberg ◽  
William F. Marzluff
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Sea Urchin ◽  
Kinase Activity ◽  
Cyclin E ◽  
Sperm Head ◽  
Mitotic Spindles ◽  
Paternal Genome ◽  
Cell Cycles ◽  
Cdk2 Activity

In somatic cells, cyclin E-cdk2 activity oscillates during the cell cycle and is required for the regulation of the G1/S transition. Cyclin E and its associated kinase activity remain constant throughout early sea urchin embryogenesis, consistent with reports from studies using several other embryonic systems. Here we have expanded these studies and show that cyclin E rapidly and selectively enters the sperm head after fertilization and remains concentrated in the male pronucleus until pronuclear fusion, at which time it disperses throughout the zygotic nucleus. We also show that cyclin E is not concentrated at the centrosomes but is associated with condensed chromosomes throughout mitosis for at least the first four cell cycles. Isolated mitotic spindles are enriched for cyclin E and cdk2, which are localized to the chromosomes. The chromosomal cyclin E is associated with active kinase during mitosis. We propose that cyclin E may play a role in the remodeling of the sperm head and re-licensing of the paternal genome after fertilization. Furthermore, cyclin E does not need to be degraded or dissociated from the chromosomes during mitosis; instead, it may be required on chromosomes during mitosis to immediately initiate the next round of DNA replication.

scholarly journals MAP kinase dependent cyclinE/cdk2 activity promotes DNA replication in early sea urchin embryos

2009 ◽  
Vol 334 (2) ◽  
pp. 383-394 ◽  
Author(s):  
J. Kisielewska ◽  
R. Philipova ◽  
J.-Y. Huang ◽  
M. Whitaker
Keyword(s):  
Dna Replication ◽  
Map Kinase ◽  
Sea Urchin ◽  
Sea Urchin Embryos ◽  
Cdk2 Activity

Inhibition by aphidicolin of cell cycle progression and DNA replication in sea urchin embryos

1979 ◽  
Vol 100 (3) ◽  
pp. 439-444 ◽  
Author(s):  
Susumu Ikegami ◽  
Shonan Amemiya ◽  
Mieko Oguro ◽  
Hiroshi Nagano ◽  
Yoshitake Mano
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Sea Urchin ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Sea Urchin Embryos

Inhibition of cysteine protease activity disturbs DNA replication and prevents mitosis in the early mitotic cell cycles of sea urchin embryos

2005 ◽  
Vol 204 (2) ◽  
pp. 693-703 ◽  
Author(s):  
Carolina Concha ◽  
Antonia Monardes ◽  
Yasmine Even ◽  
Violeta Morin ◽  
Marcia Puchi ◽  
...  
Keyword(s):  
Dna Replication ◽  
Sea Urchin ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Mitotic Cell ◽  
Cell Cycles ◽  
Sea Urchin Embryos ◽  
Cysteine Protease Activity

scholarly journals SELECTIVE INHIBITION OF GENETIC TRANSCRIPTION IN SEA URCHIN EMBRYOS

1972 ◽  
Vol 55 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Brian L. Kotzin ◽  
Robert F. Baker
Keyword(s):  
Dna Replication ◽  
Experimental Evidence ◽  
Sea Urchin ◽  
Sequence Homology ◽  
Nuclear Dna ◽  
Size Range ◽  
Selective Inhibition ◽  
Gastrula Stage ◽  
Sea Urchin Embryos ◽  
Stage Of Development

Experimental evidence suggests that exposure of sea urchin gastrulae to 5-bromodeoxyuridine (BUdR), an analog of thymidine, causes a reduction in the rate of synthesis of some RNA species usually transcribed at this stage of development. In pulse-chase experiments, 3H (in gastrula stage 8–15S nuclear DNA labeled with BUdR-3H) could not be chased, with unlabeled BUdR, into 20–60S size-range DNA; in similar experiments in which gastrulae were pulsed with thymidine-3H and then chased with unlabeled thymidine, 3H in 8–15S nuclear DNA could be extensively chased into 20–60S DNA. DNA-RNA hybridization assays indicate that gastrula stage nuclear DNA in the range of 20–60S has greater sequence homology for gastrula stage RNA-3H than does nuclear DNA of similar size-range taken from gastrulae exposed to BUdR for 1 hr. An explanation is offered for the effect of BUdR on transcription and DNA replication in sea urchin embryos.

Discontinuous DNA replication in developing sea urchin embryos

1976 ◽  
Vol 54 (1) ◽  
pp. 151-156 ◽  
Author(s):  
Hiraku Shimada ◽  
Hiroshi Terayama
Keyword(s):  
Dna Replication ◽  
Sea Urchin ◽  
Sea Urchin Embryos
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