Inhibition of cysteine protease activity disturbs DNA replication and prevents mitosis in the early mitotic cell cycles of sea urchin embryos

2005 ◽  
Vol 204 (2) ◽  
pp. 693-703 ◽  
Author(s):  
Carolina Concha ◽  
Antonia Monardes ◽  
Yasmine Even ◽  
Violeta Morin ◽  
Marcia Puchi ◽  
...  
Keyword(s):  
Dna Replication ◽  
Sea Urchin ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Mitotic Cell ◽  
Cell Cycles ◽  
Sea Urchin Embryos ◽  
Cysteine Protease Activity

scholarly journals Cysteine protease activity in the wall of abdominal aortic aneurysms

2007 ◽  
Vol 46 (6) ◽  
pp. 1260-1266 ◽  
Author(s):  
Said Abisi ◽  
Kevin G. Burnand ◽  
Matthew Waltham ◽  
Julia Humphries ◽  
Peter R. Taylor ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Abdominal Aortic Aneurysms ◽  
Aortic Aneurysms ◽  
Cysteine Protease Activity ◽  
Abdominal Aortic

scholarly journals The M Protein Is Dispensable for Maturation of Streptococcal Cysteine Protease SpeB

2005 ◽  
Vol 73 (2) ◽  
pp. 859-864 ◽  
Author(s):  
Björn Zimmerlein ◽  
Hae-Sun Park ◽  
Shaoying Li ◽  
Andreas Podbielski ◽  
P. Patrick Cleary
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Active Form ◽  
Surface Protein ◽  
M Protein ◽  
Cysteine Protease Activity ◽  
Group A ◽  
Streptococcal Pyrogenic Exotoxin B ◽  
Major Surface ◽  
Culture Supernatants

ABSTRACT The streptococcal pyrogenic exotoxin B (SpeB) is an important virulence factor of group A streptococci (GAS) with cysteine protease activity. Maturation of SpeB to a proteolytically active form was suggested to be dependent on cell-wall-anchored M1 protein, the major surface protein of GAS (M. Collin and A. Olsén, Mol. Microbiol. 36:1306-1318, 2000). Collin and Olsén showed that mutant GAS strains expressing truncated M protein secrete a conformationally different form of unprocessed SpeB with no proteolytic activity. Alternatively, we hypothesized that a truncated M protein may interfere with processing of this secreted protease, and therefore we tested cysteine protease activity in genetically defined mutant strains that express either no M protein or membrane-anchored M protein with an in-frame deletion of the AB repeat region. Measurements of SpeB activity by cleavage of a substrate n-benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride showed that the proteolytic activities in culture supernatants of both mutants were similar to those from the wild-type strain. In addition, Western blot analysis of culture supernatants showed that SpeB expression and processing to a mature form was unaffected by either deletion mutation. Therefore, we conclude that M protein is not required for maturation of the streptococcal cysteine protease SpeB.

scholarly journals Epicutaneous Administration of Papain Induces IgE and IgG Responses in a Cysteine Protease Activity-Dependent Manner

2014 ◽  
Vol 63 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Hideo Iida ◽  
Toshiro Takai ◽  
Yusuke Hirasawa ◽  
Seiji Kamijo ◽  
Sakiko Shimura ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Dependent Manner ◽  
Cysteine Protease Activity ◽  
Activity Dependent

scholarly journals Computational modelling of chromosome re-replication in mutant strains of fission yeast

2020 ◽  
Author(s):  
Béla Novák ◽  
John J Tyson
Keyword(s):  
Dna Replication ◽  
Fission Yeast ◽  
Computational Modelling ◽  
Mitotic Cell ◽  
Cyclin Dependent Kinase ◽  
Slow Increase ◽  
Cell Cycles ◽  
Mitotic Cyclin ◽  
The Mathematical Model ◽  
Insight Into

AbstractTypically cells replicate their genome only once per division cycle, but under some circumstances, both natural and unnatural, cells synthesize an overabundance of DNA, either in a disorganized fashion (‘over-replication’) or by a systematic doubling of chromosome number (‘endoreplication’). These variations on the theme of DNA replication and division have been studied in strains of fission yeast, Schizosaccharomyces pombe, carrying mutations that interfere with the function of mitotic cyclin-dependent kinase (Cdk1:Cdc13) without impeding the roles of DNA-replication licensing factor (Cdc18) and S-phase cyclin-dependent kinase (Cdk1:Cig2). Some of these mutations support endoreplication, and some over-replication. In this paper, we propose a dynamical model of the interactions among the proteins governing DNA replication and cell division in fission yeast. By computational simulations of the mathematical model, we account for the observed phenotypes of these re-replicating mutants, and by theoretical analysis of the dynamical system, we provide insight into the molecular distinctions between over-replicating and endoreplicating cells. In case of induced over-production of regulatory proteins, our model predicts that cells first switch from normal mitotic cell cycles to growth-controlled endoreplication, and ultimately to disorganized over-replication, parallel to the slow increase of protein to very high levels.

scholarly journals Novel localization and possible functions of cyclin E in early sea urchin development

2002 ◽  
Vol 115 (1) ◽  
pp. 113-121 ◽  
Author(s):  
Bradley J. Schnackenberg ◽  
William F. Marzluff
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Sea Urchin ◽  
Kinase Activity ◽  
Cyclin E ◽  
Sperm Head ◽  
Mitotic Spindles ◽  
Paternal Genome ◽  
Cell Cycles ◽  
Cdk2 Activity

In somatic cells, cyclin E-cdk2 activity oscillates during the cell cycle and is required for the regulation of the G1/S transition. Cyclin E and its associated kinase activity remain constant throughout early sea urchin embryogenesis, consistent with reports from studies using several other embryonic systems. Here we have expanded these studies and show that cyclin E rapidly and selectively enters the sperm head after fertilization and remains concentrated in the male pronucleus until pronuclear fusion, at which time it disperses throughout the zygotic nucleus. We also show that cyclin E is not concentrated at the centrosomes but is associated with condensed chromosomes throughout mitosis for at least the first four cell cycles. Isolated mitotic spindles are enriched for cyclin E and cdk2, which are localized to the chromosomes. The chromosomal cyclin E is associated with active kinase during mitosis. We propose that cyclin E may play a role in the remodeling of the sperm head and re-licensing of the paternal genome after fertilization. Furthermore, cyclin E does not need to be degraded or dissociated from the chromosomes during mitosis; instead, it may be required on chromosomes during mitosis to immediately initiate the next round of DNA replication.

Noninvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes

2007 ◽  
Vol 3 (10) ◽  
pp. 668-677 ◽  
Author(s):  
Galia Blum ◽  
Georges von Degenfeld ◽  
Milton J Merchant ◽  
Helen M Blau ◽  
Matthew Bogyo
Keyword(s):  
Optical Imaging ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Cysteine Protease Activity

Elevetad Cysteine-Protease Activity in Bronchiolitis Obliterans Syndrome

2019 ◽  
Vol 38 (4) ◽  
pp. S250
Author(s):  
C. Morrone ◽  
N. Smirnova ◽  
N. Kneidinger ◽  
H. Schiller ◽  
O. Eickelberg ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Bronchiolitis Obliterans ◽  
Bronchiolitis Obliterans Syndrome ◽  
Cysteine Protease Activity
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