Tamoxifen-inducible NaV1.8-CreERT2 recombinase activity in nociceptive neurons of dorsal root ganglia

genesis ◽  
2006 ◽  
Vol 44 (8) ◽  
pp. 364-371 ◽  
Author(s):  
Jing Zhao ◽  
Mohammed A. Nassar ◽  
Isabella Gavazzi ◽  
John N. Wood
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Nociceptive Neurons

M2-Receptor Subtype Does Not Mediate Muscarine-Induced Increases in [Ca2+]i in Nociceptive Neurons of Rat Dorsal Root Ganglia

2000 ◽  
Vol 84 (4) ◽  
pp. 1934-1941 ◽  
Author(s):  
Rainer Haberberger ◽  
Reas Scholz ◽  
Wolfgang Kummer ◽  
Michaela Kress
Keyword(s):  
Receptor Antagonist ◽  
Muscarinic Receptor ◽  
Sensory Neurons ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Receptor Subtype ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
Rt Pcr ◽  
Nociceptive Neurons

Multiple muscarinic receptor subtypes are present on sensory neurons that may be involved in the modulation of nociception. In this study we focused on the presence of the muscarinic receptor subtypes, M2 and M3 (M2R, M3R), in adult rat lumbar dorsal root ganglia (DRG) at the functional ([Ca2+]i measurement), transcriptional (RT-PCR), and translational level (immunohistochemistry). After 1 day in culture exposure of dissociated medium-sized neurons (20–35 μm diam) to muscarine was followed by rises in [Ca2+]i in 76% of the neurons. The [Ca2+]i increase was absent after removal of extracellular calcium and did not desensitize after repetitive application of the agonist. This rise in [Ca2+]i may be explained by the expression of M3R, which can induce release of calcium from internal stores via inositoltrisphospate. Indeed the effect was antagonized by the muscarinic receptor antagonist atropine as well as by the M3R antagonist, 4-diphenylacetoxy-N-(2 chloroethyl)-piperidine hydrochloride (4-DAMP). The pharmacological identification of M3R was corroborated by RT-PCR of total RNA and single-cell RT-PCR, which revealed the presence of mRNA for M3R in lumbar DRG and in single sensory neurons. In addition, RT-PCR also revealed the expression of M2R, which did not seem to contribute to the calcium changes since it was not prevented by the M2 receptor antagonist, gallamine. Immunohistochemistry demonstrated the presence of M2R and M3R in medium-sized lumbar DRG neurons that also coexpressed binding sites for the lectin I-B4, a marker for mainly cutaneous nociceptors. The occurrence of muscarinic receptors in putative nociceptive I-B4-positive neurons suggests the involvement of these acetylcholine receptors in the modulation of processing of nociceptive stimuli.

Cyclooxygenase-1 is a marker for a subpopulation of putative nociceptive neurons in rat dorsal root ganglia

2000 ◽  
Vol 12 (3) ◽  
pp. 911-920 ◽  
Author(s):  
B. Chopra ◽  
S. Giblett ◽  
J. G. Little ◽  
L. F. Donaldson ◽  
S. Tate ◽  
...  
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Nociceptive Neurons ◽  
Cyclooxygenase 1

Role of myosin Va in neuritogenesis of chick dorsal root ganglia nociceptive neurons

2013 ◽  
Vol 38 (3) ◽  
pp. 388-394 ◽  
Author(s):  
Tatiane Y. N. Kanno ◽  
Enilza M. Espreafico ◽  
Chao Yun Irene Yan
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Nociceptive Neurons ◽  
Myosin Va

Conditional gene deletion in primary nociceptive neurons of trigeminal ganglia and dorsal root ganglia

genesis ◽  
2004 ◽  
Vol 38 (3) ◽  
pp. 122-129 ◽  
Author(s):  
Nitin Agarwal ◽  
Stefan Offermanns ◽  
Rohini Kuner
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Gene Deletion ◽  
Trigeminal Ganglia ◽  
Nociceptive Neurons

scholarly journals Sensitization of Cutaneous Primary Afferents in Bone Cancer Revealed by In Vivo Calcium Imaging

Cancers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3491
Author(s):  
Larissa de Clauser ◽  
Ana P. Luiz ◽  
Sonia Santana-Varela ◽  
John N. Wood ◽  
Shafaq Sikandar
Keyword(s):  
Bone Marrow ◽  
Dorsal Root Ganglia ◽  
Calcium Imaging ◽  
Dorsal Root ◽  
Primary Afferents ◽  
Cutaneous Afferents ◽  
Retrograde Labelling ◽  
Nociceptive Neurons ◽  
In Vivo Calcium Imaging

Cancer-induced bone pain (CIBP) is a complex condition, comprising components of inflammatory and neuropathic processes, but changes in the physiological response profiles of bone-innervating and cutaneous afferents remain poorly understood. We used a combination of retrograde labelling and in vivo calcium imaging of bone marrow-innervating dorsal root ganglia (DRG) neurons to determine the contribution of these cells in the maintenance of CIBP. We found a majority of femoral bone afferent cell bodies in L3 dorsal root ganglia (DRG) that also express the sodium channel subtype Nav1.8—a marker of nociceptive neurons—and lack expression of parvalbumin—a marker for proprioceptive primary afferents. Surprisingly, the response properties of bone marrow afferents to both increased intraosseous pressure and acid were unchanged by the presence of cancer. On the other hand, we found increased excitability and polymodality of cutaneous afferents innervating the ipsilateral paw in cancer bearing animals, as well as a behavioural phenotype that suggests changes at the level of the DRG contribute to secondary hypersensitivity. This study demonstrates that cutaneous afferents at distant sites from the tumour bearing tissue contribute to mechanical hypersensitivity, highlighting these cells as targets for analgesia.

T1449 TNBS Colitis Evokes Hyperalgesic Priming of Mouse Colonic Dorsal Root Ganglia Nociceptive Neurons Involving a PKC Mechanism

2008 ◽  
Vol 134 (4) ◽  
pp. A-558
Author(s):  
Fernando Ochoa-Cortes ◽  
Francisco Bautista-Cruz ◽  
Ian Spreadbury ◽  
Stephen J. Vanner
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Nociceptive Neurons ◽  
Tnbs Colitis ◽  
Hyperalgesic Priming
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