Tissue‐specific analysis of Fgf18 gene function in palate development

2020 ◽  
Author(s):  
Minghui Yue ◽  
Yu Lan ◽  
Han Liu ◽  
Zhaoming Wu ◽  
Toru Imamura ◽  
...  
Keyword(s):  
Gene Function ◽  
Tissue Specific ◽  
Palate Development ◽  
Specific Analysis

scholarly journals A unique mouse strain expressing Cre recombinase for tissue-specific analysis of gene function in palate and kidney development

genesis ◽  
2007 ◽  
Vol 45 (10) ◽  
pp. 618-624 ◽  
Author(s):  
Yu Lan ◽  
Qingru Wang ◽  
Catherine E. Ovitt ◽  
Rulang Jiang
Keyword(s):  
Gene Function ◽  
Mouse Strain ◽  
Kidney Development ◽  
Cre Recombinase ◽  
Tissue Specific ◽  
Specific Analysis

Mechanisms of glycolytic control during facultative anaerobiosis in a marine mollusc: tissue-specific analysis of glycogen phosphorylase and fructose-2,6-bisphosphate

1988 ◽  
Vol 66 (8) ◽  
pp. 1767-1771 ◽  
Author(s):  
Kenneth B. Storey
Keyword(s):  
Glycogen Phosphorylase ◽  
Retractor Muscle ◽  
Dramatic Reduction ◽  
Metabolic Rate Depression ◽  
Tissue Specific ◽  
Marine Mollusc ◽  
Energy Needs ◽  
Specific Analysis ◽  
Anaerobic Energy ◽  
Glycolytic Control

Changes in the activity of glycogen phosphorylase and the content of fructose-2,6-bisphosphate (F-2, 6-P2) were monitored in tissues of the whelk, Busycotypus canaliculatum, over a 21-h course of environmental anoxia. Tissue-specific responses to anoxia were seen with respect to phosphorylase content: in the radular retractor muscle and foot, the content of phosphorylase a expressed rose rapidly over the initial hours of anoxia (maximal increases were 4.3- and 2.5-fold, respectively) while in the gill, content dropped 2-fold during anoxia. Phosphorylase content was modulated by two mechanisms, changes in the percentage of enzyme in the active a form and changes in the total amount (a + b) of enzyme expressed. Anoxia stimulated a dramatic reduction in F-2,6-P2 content in five tissues. In the ventricle, content fell by 224-fold with a t1/2 of only 35 min. Levels in gill, radular retractor, hepatopancreas, and kidney fell to 2.5–3.5% of control values within the first 8 h of anoxia. F-2,6-P2 content in foot muscle was not altered during anoxia. Changes in glycogen phosphorylase activities and F-2,6-P2 contents help to produce tissue-specific responses of glycolysis to environmental anoxia that acknowledge competing metabolic demands including metabolic rate depression, changes in fuel use, anaerobic energy needs, and carbohydrate use for anabolic purposes.

Tissue specific analysis of bioconversion traits in the bioenergy grass Sorghum bicolor

2013 ◽  
Vol 50 ◽  
pp. 118-130 ◽  
Author(s):  
Joshua P. Vandenbrink ◽  
Ryan E. Hammonds ◽  
Roger N. Hilten ◽  
K.C. Das ◽  
J. Michael Henson ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Tissue Specific ◽  
Specific Analysis

scholarly journals Tissue‐Specific Analysis of Pharmacological Pathways

2018 ◽  
Vol 7 (7) ◽  
pp. 453-463 ◽  
Author(s):  
Yun Hao ◽  
Kayla Quinnies ◽  
Ronald Realubit ◽  
Charles Karan ◽  
Nicholas P. Tatonetti
Keyword(s):  
Tissue Specific ◽  
Specific Analysis

scholarly journals CORP: Using transgenic mice to study skeletal muscle physiology

2020 ◽  
Vol 128 (5) ◽  
pp. 1227-1239
Author(s):  
C. Brooks Mobley ◽  
Ivan J. Vechetti ◽  
Taylor R. Valentino ◽  
John J. McCarthy
Keyword(s):  
Skeletal Muscle ◽  
Transgenic Mice ◽  
Research Program ◽  
Gene Function ◽  
Cell Biology ◽  
Muscle Physiology ◽  
Basic Information ◽  
Adult Skeletal Muscle ◽  
Tissue Specific ◽  
Muscle Research

The development of tissue-specific inducible transgenic mice has provided a powerful tool to study gene function and cell biology in almost any tissue of interest at any given time within the animal’s life. The purpose of this review is to describe how to use two different inducible transgenic systems, the Cre-loxP system and the Tet-ON/OFF system, that can be used to study skeletal muscle physiology. Myofiber- and satellite cell-specific Cre-loxP transgenic mice are described as is how these mice can be used to knockout a gene of interest or to deplete satellite cells in adult skeletal muscle, respectively. A myofiber-specific Tet-ON system is described as is how such mice can be used to overexpress a gene of interest or to label myonuclei. How to effectively breed and genotype the transgenic mice are also described in detail. The hope is this review will provide the basic information necessary to facilitate the incorporation of tissue-specific inducible transgenic mice into a skeletal muscle research program.

Missing mitochondrial Mpv17 gene function induces tissue-specific cell-death pathway in the degenerating inner ear

2012 ◽  
Vol 347 (2) ◽  
pp. 343-356 ◽  
Author(s):  
Angela-Maria Meyer zum Gottesberge ◽  
Thomas Massing ◽  
Stefan Hansen
Keyword(s):  
Cell Death ◽  
Inner Ear ◽  
Gene Function ◽  
Specific Cell ◽  
Tissue Specific ◽  
Cell Death Pathway

scholarly journals Illuminating a plant’s tissue-specific metabolic diversity using computational metabolomics and information theory

2016 ◽  
Vol 113 (47) ◽  
pp. E7610-E7618 ◽  
Author(s):  
Dapeng Li ◽  
Sven Heiling ◽  
Ian T. Baldwin ◽  
Emmanuel Gaquerel
Keyword(s):  
Information Theory ◽  
Secondary Metabolism ◽  
Gene Function ◽  
Tissue Level ◽  
Two Dimensions ◽  
Molecular Networks ◽  
Specific Gene ◽  
Theory Analysis ◽  
Tissue Specific ◽  
Metabolite Diversity

Secondary metabolite diversity is considered an important fitness determinant for plants’ biotic and abiotic interactions in nature. This diversity can be examined in two dimensions. The first one considers metabolite diversity across plant species. A second way of looking at this diversity is by considering the tissue-specific localization of pathways underlying secondary metabolism within a plant. Although these cross-tissue metabolite variations are increasingly regarded as important readouts of tissue-level gene function and regulatory processes, they have rarely been comprehensively explored by nontargeted metabolomics. As such, important questions have remained superficially addressed. For instance, which tissues exhibit prevalent signatures of metabolic specialization? Reciprocally, which metabolites contribute most to this tissue specialization in contrast to those metabolites exhibiting housekeeping characteristics? Here, we explore tissue-level metabolic specialization in Nicotiana attenuata, an ecological model with rich secondary metabolism, by combining tissue-wide nontargeted mass spectral data acquisition, information theory analysis, and tandem MS (MS/MS) molecular networks. This analysis was conducted for two different methanolic extracts of 14 tissues and deconvoluted 895 nonredundant MS/MS spectra. Using information theory analysis, anthers were found to harbor the most specialized metabolome, and most unique metabolites of anthers and other tissues were annotated through MS/MS molecular networks. Tissue–metabolite association maps were used to predict tissue-specific gene functions. Predictions for the function of two UDP-glycosyltransferases in flavonoid metabolism were confirmed by virus-induced gene silencing. The present workflow allows biologists to amortize the vast amount of data produced by modern MS instrumentation in their quest to understand gene function.

scholarly journals New strains for tissue-specific RNAi studies in Caenorhabditis elegans

2018 ◽  
Author(s):  
Jason S. Watts ◽  
Henry F. Harrison ◽  
Shizue Omi ◽  
Quentin Guenthers ◽  
James Dalelio ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Caenorhabditis Elegans ◽  
Gene Function ◽  
Target Genes ◽  
Resistant Mutant ◽  
Knockout Mutant ◽  
Tissue Specific ◽  
Double Stranded Rna ◽  
C Elegans ◽  
Insight Into

AbstractRNA interference is a powerful tool for dissecting gene function. In Caenorhabditis elegans, ingestion of double stranded RNA causes strong, systemic knockdown of target genes. Further insight into gene function can be revealed by tissue-specific RNAi techniques. Currently available tissue-specific C. elegans strains rely on rescue of RNAi function in a desired tissue or cell in an otherwise RNAi deficient genetic background. We attempted to assess the contribution of specific tissues to polyunsaturated fatty acid (PUFA) synthesis using currently available tissue-specific RNAi strains. We discovered that rde-1 (ne219), a commonly used RNAi-resistant mutant strain, retains considerable RNAi capacity against RNAi directed at PUFA synthesis genes. By measuring changes in the fatty acid products of the desaturase enzymes that synthesize PUFAs, we found that the before mentioned strain, rde-1 (ne219) and the reported germline only RNAi strain, rrf-1 (pk1417) are not appropriate genetic backgrounds for tissue-specific RNAi experiments. However, the knockout mutant rde-1 (ne300) was strongly resistant to dsRNA induced RNAi, and thus is more appropriate for construction of a robust tissue-specific RNAi strains. Using newly constructed strains in the rde-1(null) background, we found considerable desaturase activity in intestinal, epidermal, and germline tissues, but not in muscle. The RNAi-specific strains reported in this study will be useful tools for C. elegans researchers studying a variety of biological processes.

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