scholarly journals Use of adenovirus for ectopic gene expression inXenopus: Chicken dapper genes are versatile markers for mesodermal tissues, embryonic muscle stem cells, neural crest cells, and neurogenic placodes

2009 ◽  
Vol 238 (7) ◽  
pp. spcone-spcone
Author(s):  
Lúcia Elvira Alvares ◽  
Farrah Leigh Winterbottom ◽  
Débora Rodrigues Sobreira ◽  
José Xavier-Neto ◽  
Frank Richard Schubert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Muscle Stem Cells ◽  
Ectopic Gene Expression ◽  
Embryonic Muscle

Chicken dapper genes are versatile markers for mesodermal tissues, embryonic muscle stem cells, neural crest cells, and neurogenic placodes

2009 ◽  
Vol 238 (5) ◽  
pp. 1166-1178 ◽  
Author(s):  
Lúcia Elvira Alvares ◽  
Farrah Leigh Winterbottom ◽  
Débora Rodrigues Sobreira ◽  
José Xavier-Neto ◽  
Frank Richard Schubert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Muscle Stem Cells ◽  
Embryonic Muscle

Chicken dapper genes are versatile markers for mesodermal tissues, embryonic muscle stem cells, neural crest cells, and neurogenic placodes

2009 ◽  
Vol 238 (11) ◽  
pp. 2948-2948
Author(s):  
Lúcia Elvira Alvares ◽  
Farrah Leigh Winterbottom ◽  
Erika Cristina Jorge ◽  
Débora Rodrigues Sobreira ◽  
José Xavier-Neto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Muscle Stem Cells ◽  
Embryonic Muscle

scholarly journals SV40T reprograms Schwann cells into stem-like cells that can re-differentiate into terminal nerve cells

2021 ◽  
Author(s):  
Rui-fang Li ◽  
Guo-xin Nan ◽  
Dan Wang ◽  
Chang Gao ◽  
Juan Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Stem Cells ◽  
Neural Crest ◽  
Schwann Cells ◽  
Early Development ◽  
Glial Cells ◽  
Embryonic Stem ◽  
Specific Effect ◽  
Neural Crest Cells

Background: The specific effect of SV40T on neurocytes has been rarely investigated by the researchers. We transfected Schwann cells (SCs) that did not have differentiation ability with MPH 86 plasmid containing SV40T in order to explore the effects of SV40T on Schwann cells.Methods: SCs were transfected with MPH 86 plasmid carrying the SV40T gene and cultured in different media, as well as co-cultured with neural stem cells (NSCs). In our study, SCs overexpressing SV40T were defined as SV40T-SCs. The proliferation of these cells was detected by WST-1, and the expression of different biomarkers was analyzed by qPCR and immunohistochemistry. Results: SV40T induced the characteristics of NSCs, such as the ability to grow in suspension, form spheroid colonies and proliferate rapidly, in the SCs, which were reversed by knocking out SV40T by the Flip-adenovirus. In addition, SV40T upregulated the expressions of neural crest-associated markers Nestin, Pax3 and Slug, and down-regulated S100b as well as the markers of mature SCs MBP, GFAP and Olig1/2. These cells also expressed NSC markers like Nestin, Sox2, CD133 and SSEA-1, as well as early development markers of embryonic stem cells (ESCs) like BMP4, c-Myc, OCT4 and Gbx2. Co-culturing with NSCs induced differentiation of the SV40T-SCs into neuronal and glial cells. Conclusions: SV40T reprograms Schwann cells to stem-like cells at the stage of neural crest cells (NCCs) that can differentiate to neurocytes.

scholarly journals RB2/p130 ectopic gene expression in neuroblastoma stem cells: evidence of cell-fate restriction and induction of differentiation

2001 ◽  
Vol 360 (3) ◽  
pp. 569 ◽  
Author(s):  
Francesco P. JORI ◽  
Umberto GALDERISI ◽  
Elena PIEGARI ◽  
Gianfranco PELUSO ◽  
Marilena CIPOLLARO ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Fate ◽  
Ectopic Gene Expression ◽  
Induction Of Differentiation ◽  
Fate Restriction

scholarly journals Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells

2019 ◽  
Vol 28 (1) ◽  
pp. 28-43 ◽  
Author(s):  
Shota Fujii ◽  
Satoru Yoshida ◽  
Emi Inagaki ◽  
Shin Hatou ◽  
Kazuo Tsubota ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neural Crest Cells ◽  
Immunological Properties ◽  
Induced Pluripotent

289 COOPERATIVE EXPRESSION OF PLURIPOTENCY-RELATED GENES AND NEURAL CREST MARKER GENES IN PORCINE GFP-TRANSGENIC SKIN-DERIVED PROGENITORS

2009 ◽  
Vol 21 (1) ◽  
pp. 241
Author(s):  
M. T. Zhao ◽  
C. S. Isom ◽  
J. G. Zhao ◽  
Y. H. Hao ◽  
J. Ross ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Neural Crest ◽  
Therapeutic Potential ◽  
Marker Gene ◽  
Stem Cell Marker ◽  
Marker Genes ◽  
Specific Marker ◽  
Marker Gene Expression ◽  
Neural Crest Origin

Recently neural crest derived multipotent progenitors from skin have attracted much attention as the skin may provide an accessible, autologous source of stem cells available with therapeutic potential (Toma JG et al. 2001 Nat. Cell Biol. 3, 778–784). The multipotent property of stem cells could be tracked back to the expression of specific marker genes that are exclusively expressed in multipotent stem cells rather than any other types of differentiated cells. Here we demonstrate the property of multipotency and neural crest origin of porcine GFP-transgenic skin derived progenitors (termed pSKP) in vitro by marker gene expression analysis. The pSKP cells were isolated from the back skin of GFP transgenic fetuses by serum-free selection culture in the presence of EGF (20 ng mL–1) and bFGF (40 ng mL–1), and developed into spheres in 1–2 weeks (Dyce PW et al. 2004 Biochem. Biophy. Res. Commun. 316, 651–658). Three groups of RT-PCR primers were used on total RNA from purified pSKP cells: pluripotency related genes (Oct4, Sox2, Nanog, Stat3), neural crest marker genes (p75NGFR, Slug, Twist, Pax3, Sox9, Sox10) and lineage specific genes (GFAP, tubulin β-III, leptin). Expression of both pluripotency related genes and neural crest marker genes were detected in undifferentiated pSKP cells. In addition, transcripts for fibronectin, vimentin and nestin (neural stem cell marker) were also present. The percentage of positive cells for Oct4, fibronection and vimentin were 12.3%, 67.9% and 53.7% respectively. Differentiation assays showed the appearance of tubulin β-III positive (39.4%) and GFAP-positive (42.6%) cells in cultures by immunocytochemistry, which share the characteristics of neurons and glial cells, respectively. Thus, we confirm the multiple lineage potentials and neural crest origin of pSKP cells in the level of marker gene expression. This work was funded by National Institutes of Health National Center for Research Resources RR013438.

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